Saccharification of Delignified Sugarcane Bagasse for Lactic Acid Fermentation Using Lactobacillus sp.

Delignified sugarcane bagasse (SCB) by solvent extraction was carried out at moderate condition (90°C, 4 h) in the presence of acid catalyst. To investigate the dissolution of lignin into solvent, different solvents were utilized during lignin extraction process. Delignified SCB was further hydrolyzed by cell wall degrading enzyme complexes prior to sugar determination. The results showed that n-butanol was the most promising solvent enhancing lignin dissolution which in turn led to highest yield of glucose (63.1% based on treated SCB) and no xylose was detected in hydrolysate. The lignin extraction by n-butanol in the presence of H2SO4 and subsequent saccharification process were then scaled up for lactic acid production. The maximum lactic acid was obtained at 25.7 ± 0.2 g/l from L. casei fermentation after 96 hours when sugarcane bagasse hydrolysate containing 25.6±1.4 g/l initial glucose concentration was used as substrate.

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Statistical Optimization of Alkali Pretreatment to Improve Sugars Recovery from Spent Coffee Grounds and Utilization in Lactic Acid Fermentation

Processes ◽

10.3390/pr9030494 ◽

2021 ◽

Vol 9 (3) ◽

pp. 494 ◽

Cited By ~ 1

Author(s):

Kang Hyun Lee ◽

Ye Won Jang ◽

Jeongho Lee ◽

Seunghee Kim ◽

Chulhwan Park ◽

...

Keyword(s):

Lactic Acid ◽

Enzymatic Hydrolysis ◽

Lactic Acid Production ◽

Complex Loading ◽

Lactic Acid Fermentation ◽

Optimum Conditions ◽

Spent Coffee Grounds ◽

Alkali Pretreatment ◽

Acid Fermentation ◽

Coffee Grounds

Biorefinery, which utilizes carbon-neutral biomass as a resource, is attracting attention as a significant alternative in a modern society confronted with climate change. In this study, spent coffee grounds (SCGs) were used as the feedstock for lactic acid fermentation. In order to improve sugar conversion, alkali pretreatment was optimized by a statistical method, namely response surface methodology (RSM). The optimum conditions for the alkali pretreatment of SCGs were determined as follows: 75 °C, 3% potassium hydroxide (KOH) and a time of 2.8 h. The optimum conditions for enzymatic hydrolysis of pretreated SCGs were determined as follows: enzyme complex loading of 30-unit cellulase, 15-unit cellobiase and 50-unit mannanase per g biomass and a reaction time of 96 h. SCG hydrolysates were used as the carbon source for Lactobacillus cultivation, and the conversions of lactic acid by L. brevis ATCC 8287 and L. parabuchneri ATCC 49374 were 40.1% and 55.8%, respectively. Finally, the maximum lactic acid production by L. parabuchneri ATCC 49374 was estimated to be 101.2 g based on 1000 g of SCGs through the optimization of alkali pretreatment and enzymatic hydrolysis.

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Development of Four Unit Processes for Biobased PLA Manufacturing

ISRN Polymer Science ◽

10.5402/2012/938261 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 4

Author(s):

Chae Hwan Hong ◽

Si Hwan Kim ◽

Ji-Yeon Seo ◽

Do Suck Han

Keyword(s):

Lactic Acid ◽

Ring Opening ◽

Lactic Acid Production ◽

Scale Up ◽

Catalyst System ◽

Ring Opening Polymerization ◽

Acid Fermentation ◽

Manufacturing Method ◽

Renewable Biomass ◽

Microbial Productivity

Polylactide (PLA), which is one of the most important biocompatible polyesters that are derived from annually renewable biomass such as corn and sugar beets, has attracted much attention for automotive parts application. The manufacturing method of PLA is the ring-opening polymerization of the dimeric cyclic ester of lactic acid, lactide. For the stereocomplex PLA, we developed the four unit processes, fermentation, separation, lactide conversion, and polymerization. Fermentation of sugars to D-lactic acid is little studied, and its microbial productivity is not well known. Therefore, we investigated D-lactic acid fermentation with a view to obtaining the strains capable of producing D-lactic acid, and we got a maximum lactic acid production 60 g/L. Lactide is prepared by a two-step process: first, the lactic acid is converted into oligo(lactic acid) by a polycondensation reaction; second, the oligo(lactic acid) is thermally depolymerized to form the cyclic lactide via an unzipping mechanism. Through catalyst screening test for polycondensation and depolymerization reactions, we got a new method which shortens the whole reaction time 50% the level of the conventional method. Poly(L-lactide) was obtained from the ring-opening polymerization of L-lactide. We investigated various catalysts and polymerization conditions. Finally, we got the best catalyst system and the scale-up technology.

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Recycling Of Peneapple Waste Using Lactobacillus Delbroeckii to Lactic Acid

REAKTOR ◽

10.14710/reaktor.5.2.79-83 ◽

2017 ◽

Vol 5 (2) ◽

pp. 79

Author(s):

Abdullah Abdullah ◽

H. B. Mat

Keyword(s):

Lactic Acid ◽

Yeast Extract ◽

Lactic Acid Production ◽

Lactobacillus Delbrueckii ◽

Raw Material ◽

Acid Fermentation ◽

Fermentation Time ◽

Pineapple Waste ◽

Fermentation Liquid ◽

Initial Ph

The liquid pineapple waste contain mainly sucrose, glucose, fructose, and other nutrients. It therefore can potentiall be used as carbon source for organic acid fermentation. Recently, lactic acid has been considered to be an important raw material for production of biodegradadable lactace polymer. The experiments weree carried out in shake flash fermentation using lactobacillus delbroeckii. Effect of some parameters such as temperature, initial Ph, initial substrate concentration, yeast extract concentration and fermentation time to the yield have been studied. The highest yield was 85.65% achieved at 40 0C, PH 6.00, 52.2 g/l sugar concentration with 5 g/l yeast extract. There was no significant increasing in lactic acid production was observed if supplementation of yeast extract above 10%.Keyword : lactic acid fermentation, liquid pineapple waste, lactobacillus delbrueckii

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Synthesis of lactic acid from sugar palm trunk waste (Arenga pinnata): Preliminary hydrolysis and fermentation studies

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210559 ◽

2020 ◽

Vol 21 (5) ◽

Author(s):

WHINY HARDIYATI ERLIANA ◽

Tri Widjaja ◽

ALI ALTWAY ◽

LILY PUDJIASTUTI

Keyword(s):

Lactic Acid ◽

Lignin Content ◽

Agricultural Waste ◽

Lactic Acid Production ◽

Lactobacillus Rhamnosus ◽

Lactobacillus Brevis ◽

Reducing Sugar ◽

Cellulose Content ◽

Acid Pretreatment ◽

Acid Fermentation

Abstract. Erliana WH, Widjaja T, Altway A, Pudjiastuti L. 2020. Synthesis of lactic acid from sugar palm trunk waste (Arenga pinnata): Hydrolysis and fermentation studies. Biodiversitas 21: 2281-2288. The increasing problems of global energy and the environment are the main reasons for developing products with new techniques through green methods. Sugar palm trunk waste (SPTW) has potential as agricultural waste because of its abundant availability, but it is not used optimally. This study was aimed to determine the effect of various microorganisms on increasing lactic acid production by controlling pH and temperature conditions in the fermentation process. SPTW contains 43.88% cellulose, 7.24% hemicellulose, and 33.24% lignin. The lignin content in SPTW can inhibit reducing sugar formation; the pretreatment process should remove this content. In the study, the pretreatment process was conducted using acid-organosolv. In the acid pretreatment, 0.2 M H2SO4 was added at 120oC for 40 minutes; organosolv pretreatment using 30% ethanol (v/v) at 107oC for 33 minutes was able to increase cellulose content by 56.33% and decrease lignin content by 27.09%. The pretreatment was followed by an enzymatic hydrolysis process with a combination of commercial cellulase enzymes from Aspergillus niger (AN) and Trichoderma reesei (TR), with variations of 0:1, 1:0, 1:1, 1:2 and 2:1. The best reducing sugar concentration was obtained with an AN: TR ratio of 1:2 to form reducing sugar from cellulose. Subsequently, lactic acid fermentation was carried out using lactic acid bacteria at 37oC and pH 6 incubated for 48 hours. The highest lactic acid concentration (33.292 g/L) was obtained using a mixed culture of Lactobacillus rhamnosus and Lactobacillus brevis to convert reducing sugar become lactic acid.

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Simultaneous saccharification and lactic acid fermentation of the cellulosic fraction of municipal solid waste using Bacillus smithii

Biotechnology Letters ◽

10.1007/s10529-020-03049-y ◽

2020 ◽

Author(s):

Micaela G. Chacón ◽

Christopher Ibenegbu ◽

David J. Leak

Keyword(s):

Lactic Acid ◽

Municipal Solid Waste ◽

Solid Waste ◽

Lactic Acid Production ◽

Lactic Acid Fermentation ◽

Potential Candidate ◽

Acid Fermentation ◽

Promising Candidate ◽

Cellulosic Pulp ◽

Simultaneous Saccharification

Abstract Objective A primary drawback to simultaneous saccharification and fermentation (SSF) processes is the incompatibility of the temperature and pH optima for the hydrolysis and fermentation steps—with the former working best at 50–55 °C and pH 4.5–5.5. Here, nine thermophilic Bacillus and Parageobacillus spp. were evaluated for growth and lactic acid fermentation at high temperature and low pH. The most promising candidate was then carried forward to demonstrate SSF using the cellulosic fraction from municipal solid waste (MSW) as a feedstock. Results B. smithii SA8Eth was identified as the most promising candidate and in a batch SSF maintained at 55 °C and pH 5.0, using a cellulase dose of 5 FPU/g glucan, it produced 5.1 g/L lactic acid from 2% (w/v) MSW cellulosic pulp in TSB media. Conclusion This work has both scientific and industrial relevance, as it evaluates a number of previously untrialled bacterial hosts for their compatibility with lignocellulosic SSF for lactic acid production and successfully identifies B. smithii as a potential candidate for such a process.

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Assessment of multiple pretreatment strategies for 2G L-lactic acid production from sugarcane bagasse

Biomass Conversion and Biorefinery ◽

10.1007/s13399-020-01163-5 ◽

2020 ◽

Author(s):

Ketaki Nalawade ◽

Paharika Saikia ◽

Shuvashish Behera ◽

Kakasaheb Konde ◽

Sanjay Patil

Keyword(s):

Lactic Acid ◽

Sugarcane Bagasse ◽

Acid Production ◽

Lactic Acid Production

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Synthesis and initial screening of lactate dehydrogenase inhibitor activity of 1,3-benzodioxole derivatives

Scientific Reports ◽

10.1038/s41598-020-77056-4 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Dicky Annas ◽

Se-Yun Cheon ◽

Mohammad Yusuf ◽

Sung-Jin Bae ◽

Ki-Tae Ha ◽

...

Keyword(s):

Lactic Acid ◽

Lactate Dehydrogenase ◽

Cancer Cells ◽

Aerobic Glycolysis ◽

Dominant Role ◽

Lactic Acid Production ◽

Human Pancreatic Cancer ◽

Drug Candidate ◽

Acid Fermentation ◽

High Level

AbstractCancer is one of the main causes of mortality in the world. Many cancer cells produce ATP through high-level lactic acid fermentation catalyzed by lactate dehydrogenase (LDH), which converts pyruvic acid to lactic acid. LDH plays a dominant role in the Warburg effect, wherein aerobic glycolysis is favored over oxidative phosphorylation. Due to the high lactic acid production level in cancer cells, LDH-targeting could be a potential cancer treatment strategy. A few approaches, such as drug treatment, reportedly inhibited LDH activity. In this study, we describe new 1,3-benzodioxole derivatives that might be potential small molecule candidates for LDHA inhibition. The synthesis was carried out by trans-esterification between aryl ester and alcohol groups from piperonyl alcohol. Compounds 2 and 10 exhibited a selective LDHA IC50 value of 13.63 µM and 47.2 µM, respectively. Whereas only compound 10 showed significant cytotoxicity in several lines of cancer cells, especially in human pancreatic cancer PANC-1 cells. These synthesized compounds possess 2 aromatic rings and –CF3 moiety, which expectedly contributes to LDHA inhibition. The presented products have the potential to become a promising LDHA inhibitor drug candidate.

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A Novel Way for Whey: Cheese Whey Fermentation Produces an Effective and Environmentally-Safe Alternative to Chlorine

Applied Sciences ◽

10.3390/app9142800 ◽

2019 ◽

Vol 9 (14) ◽

pp. 2800 ◽

Cited By ~ 1

Author(s):

Maria S. Santos ◽

Patrícia Fradinho ◽

Sandro Martins ◽

Ana G. Lima ◽

Ricardo M. S. Boavida Ferreira ◽

...

Keyword(s):

Lactic Acid ◽

Cheese Whey ◽

Low Cost ◽

Lactic Acid Production ◽

Organic Content ◽

Organic Load ◽

Microbial Quality ◽

Acid Fermentation ◽

Safe Alternative ◽

Environmentally Safe

Cheese whey has been described as an environmental hazard due to its high organic content. Although it has been suggested that whey can be used as food disinfectant, it continues to pose an environmental problem because it still contains a high organic load. Here, we aimed to develop a low-cost, scalable fermentation protocol to produce a disinfectant from dairy waste that has very little organic content and high levels of lactic acid. Fermentation was achieved with industrial whey from ewe, goat, and cow’s milk, using a specific mesophilic-lactic acid bacteria starter mix over 120 h, which yielded the highest lactic acid production and the lowest lactose content. Antibacterial activity was observed against Listeria monocytogenes, Salmonella enterica, and Escherichia coli O157:H7, plus a total of thirteen other food pathogenic and spoilage strains, and antibacterial activities were determined to be highest after 120 h. We further validated this whey’s application as a disinfectant in shredded lettuce and compared its efficacy to that of chlorine, evaluating microbial quality, texture, color, and sensory perception, pH, and O2 and CO2 determinations. Results showed that not only was microbial quality better when using our whey solution (p < 0.05), but also the quality indicators for whey were statistically similar to those treated with chlorine. Hence, our work validates the use of an industrial waste whey as a low-cost, efficient, and environmentally safe disinfectant, with potential applications for minimally processed foodstuffs as an alternative to chlorine.

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