Genotypic variation in cold tolerance of 18 Ethiopian rice cultivars in relation to their reproductive morphology

Given that an effective combined foliar application of iodine (I), selenium (Se), and zinc (Zn) would be farmer friendly, compared to a separate spray of each micronutrient, for the simultaneous biofortification of grain crops, we compared effectiveness of foliar-applied potassium iodate (KIO3, 0.05%), sodium selenate (Na2SeO4, 0.0024%), and zinc sulfate (ZnSO4∙7H2O, 0.5%), separately and in their combination (as cocktail) for the micronutrient biofortification of four Basmati cultivars of rice (Oryza sativa L.). Foliar-applied, each micronutrient or their cocktail did not affect rice grain yield, but grain yield varied significantly among rice cultivars. Irrespective of foliar treatments, the brown rice of cv. Super Basmati and cv. Kisan Basmati had substantially higher concentration of micronutrients than cv. Basmati-515 and cv. Chenab Basmati. With foliar-applied KIO3, alone or in cocktail, the I concentration in brown rice increased from 12 to 186 µg kg−1. The average I concentration in brown rice with foliar-applied KIO3 or cocktail was 126 μg kg−1 in cv. Basmati-515, 160 μg kg−1 in cv. Chenab Basmati, 153 μg kg−1 in cv. Kisan Basmati, and 306 μg kg−1 in cv. Super Basmati. Selenium concentration in brown rice increased from 54 to 760 µg kg−1, with foliar-applied Na2SeO4 individually and in cocktail, respectively. The inherent Zn concentration in rice cultivars ranged between 14 and 19 mg kg−1 and increased by 5–6 mg Zn per kg grains by foliar application of ZnSO4∙7H2O and cocktail. The results also showed the existence of genotypic variation in response to foliar spray of micronutrients and demonstrated that a foliar-applied cocktail of I, Se, and Zn could be an effective strategy for the simultaneous biofortification of rice grains with these micronutrients to address the hidden hunger problem in human populations.

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Genotypic Variation in Rice Cold Tolerance Responses during Reproductive Growth as a Function of Water Temperature during Vegetative Growth

Crop Science ◽

10.2135/cropsci2010.05.0300 ◽

2011 ◽

Vol 51 (1) ◽

pp. 290-297 ◽

Cited By ~ 20

Author(s):

Hiroyuki Shimono ◽

Ayako Ishii ◽

Eiji Kanda ◽

Mitsuru Suto ◽

Kuniaki Nagano

Keyword(s):

Water Temperature ◽

Cold Tolerance ◽

Vegetative Growth ◽

Genotypic Variation ◽

Reproductive Growth

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Minimising cold damage during reproductive development among temperate rice genotypes. II. Genotypic variation and flowering traits related to cold tolerance screening

Australian Journal of Agricultural Research ◽

10.1071/ar05186 ◽

2006 ◽

Vol 57 (1) ◽

pp. 89 ◽

Cited By ~ 23

Author(s):

T. C. Farrell ◽

K. M. Fox ◽

R. L. Williams ◽

S. Fukai ◽

L. G. Lewin

Keyword(s):

Low Temperature ◽

Cold Tolerance ◽

Field Experiments ◽

Cold Water ◽

Genotypic Variation ◽

Pollen Grains ◽

Microspore Development ◽

Spikelet Sterility ◽

Temperature Conditions ◽

Anther Length

Low temperature during microspore development increases spikelet sterility and reduces grain yield in rice (Oryza sativa L.). The objectives of this study were to determine genotypic variation in spikelet sterility in the field in response to low temperature and then to examine the use of physio-morphological traits at flowering to screen for cold tolerance. Multiple-sown field experiments were conducted over 4 consecutive years in the rice-growing region of Australia to increase the likelihood of encountering low temperature during microspore development. More than 50 cultivars of various origins were evaluated, with 7 cultivars common to all 4 years. The average minimum temperature for 9 days during microspore development was used as a covariate in the analysis to compare cultivars at a similar temperature. The low-temperature conditions in Year 4 identified cold-tolerant cultivars such as Hayayuki and HSC55 and susceptible cultivars such as Sasanishiki and Doongara. After low temperature conditions, spikelet sterility was negatively correlated with the number of engorged pollen grains, anther length, anther area, anther width, and stigma area. The number of engorged pollen grains and anther length were found to be facultative traits as their relationships with spikelet sterility were identified only after cold water exposure and did not exist under non-stressed conditions.

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Gene-Coexpression Network Analysis Identifies Specific Modules and Hub Genes Related to Cold Stress in Rice

10.21203/rs.3.rs-737261/v1 ◽

2021 ◽

Author(s):

Zhichi Zeng ◽

Sichen Zhang ◽

Wenyan Li ◽

Baoshan Chen ◽

Wenlan Li

Keyword(s):

Network Analysis ◽

Low Temperature ◽

Cold Stress ◽

Cold Tolerance ◽

Temperature Stress ◽

Low Temperature Stress ◽

Rice Cultivars ◽

Hub Genes ◽

New Genes ◽

Short Period

Abstract Background: When plants are subjected to cold stress, they undergo a series of molecular and physiological changes to protect themselves from injury. Indica cultivars can usually withstand only mild cold stress in a relatively short period. Hormone-mediated defence response plays an important role in cold stress. Weighted gene co-expression network analysis (WGCNA) is a very useful tool for studying the correlation between genes, identifying modules with high phenotype correlation, and identifying Hub genes in different modules. Many studies have elucidated the molecular mechanisms of cold tolerance in different plants, but little information about the recovery process after cold stress is available.Results: To understand the molecular mechanism of cold tolerance in rice, we performed comprehensive transcriptome analyses during cold treatment and recovery stage in two cultivars of near-isogenic lines (9311 and DC907). Twelve transcriptomes in two rice cultivars were determined. A total of 2509 new genes were predicted by fragment splicing and assembly, and 7506 differentially expressed genes were identified by pairwise comparison. A total of 26 modules were obtained by expression-network analysis, 12 of which were highly correlated with cold stress or recovery treatment. We further identified candidate Hub genes associated with specific modules and analysed their regulatory relationships based on coexpression data. Results showed that various plant-hormone regulatory genes acted together to protect plants from physiological damage under short-term low-temperature stress. We speculated that this may be common in rice. Under long-term cold stress, rice improved the tolerance to low-temperature stress by promoting autophagy, sugar synthesis, and metabolism.Conclusion: Through WGCNA analysis at the transcriptome level, we provided a potential regulatory mechanism for the cold stress and recovery of rice cultivars and identified candidate central genes. Our findings provided an important reference for the future cultivation of rice strains with good tolerance.

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Genotypic variation among 20 rice cultivars/landraces in response to cadmium stress grown locally in West Bengal, India

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2020.01.019 ◽

2020 ◽

Vol 148 ◽

pp. 193-206 ◽

Cited By ~ 7

Author(s):

Falguni Barman ◽

Snehalata Majumdar ◽

Shahira Helal Arzoo ◽

Rita Kundu

Keyword(s):

West Bengal ◽

Genotypic Variation ◽

Cadmium Stress ◽

Rice Cultivars

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Transcriptome sequencing and iTRAQ of different rice cultivars provide insight into molecular mechanisms of cold-tolerance response in japonica rice

10.21203/rs.2.20308/v2 ◽

2020 ◽

Author(s):

Yan Jia ◽

Hualong Liu ◽

Zhaojun Qu ◽

Jin Wang ◽

Xinpeng Wang ◽

...

Keyword(s):

Cold Stress ◽

Cold Tolerance ◽

Nitrogen Metabolism ◽

N Uptake ◽

Differentially Expressed ◽

Japonica Rice ◽

Rice Cultivars ◽

Gene And Protein Expression ◽

Cold Tolerant ◽

Cold Sensitive

Abstract Background: Rice ( Oryza sativa L.), one of the most important crops cultivated in both tropical and temperate regions, has a high sensitivity to cold stress. Chilling stress limits N uptake and nitrogen metabolism in rice. To identify the genes and pathways involved in cold tolerance, specifically within the nitrogen metabolism pathway, we compared gene and protein expression differences between a cold-tolerant cultivar, Dongnong428 (DN), and a cold-sensitive cultivar, Songjing10 (SJ). Results: Using isobaric tags for relative or absolute quantification (iTRAQ) with high-throughput mRNA sequencing (RNA-seq) techniques, we identified 5,549 genes and 450 proteins in DN and 6,145 genes and 790 proteins in SJ, that were differentially expressed during low water temperature (T w ) treatment. There were 354 transcription factor (TF) genes (212 down, 142 up), and 366 TF genes (220 down, 146 up), including 47 gene families, differentially expressed in the DN under control (CKDN) vs. DN under low-T w (D15DN) and CKSJ vs. D15SJ, respectively. Genes related to rice cold-related biosynthesis pathways, particularly the mitogen-activated protein kinase (MAPK) signaling pathway, zeatin biosynthesis, and plant hormone signal transduction pathways, were significantly differentially expressed in both rice cultivars. Differentially expressed proteins (DEPs) related to rice cold-related biosynthesis pathways and particularly glutathione metabolism were significantly differentially expressed in both rice cultivars. Transcriptome and proteome analysis of the nitrogen metabolism pathways showed that major genes and proteins that participated in γ-aminobutyric acid (GABA) and glutamine synthesis were downregulated. Conclusion: Under cold stress conditions during reproductive growth, genes and proteins related to the biosynthesis pathways of cold stress were significantly differentially expressed in DN and SJ. The present study confirmed the known cold stress-associated genes and identified a number of putative new cold-responsive genes. We also found that translational regulation under cold stress plays an important role in cold-tolerant DN. Low-T w treatments affected N uptake and N metabolism in rice, and promoted Glu metabolism, and the synthesis of ornithine and proline in cold-sensitive SJ.

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