A successful pregnancy and live birth after intracytoplasmic sperm injection with globozoospermic sperm and electrical oocyte activation

2009 ◽  
Vol 92 (6) ◽  
pp. 2037.e5-2037.e9 ◽  
Author(s):  
Akiyoshi Egashira ◽  
Masao Murakami ◽  
Kosuke Haigo ◽  
Toshitaka Horiuchi ◽  
Takeshi Kuramoto
Keyword(s):  
Live Birth ◽  
Intracytoplasmic Sperm Injection ◽  
Oocyte Activation ◽  
Successful Pregnancy

scholarly journals The effect of cult-active medium on pregnancy outcomes after intracytoplasmic sperm injection in azoospermic men: A case-control study

2022 ◽  
pp. 1059-1066
Author(s):  
Elham Asa ◽  
Rahil Janatifar ◽  
Seyedeh Saeideh Sahraei ◽  
Atefeh Verdi ◽  
Naser Kalhor
Keyword(s):  
Active Medium ◽  
Live Birth ◽  
Intracytoplasmic Sperm Injection ◽  
Embryo Quality ◽  
Obstructive Azoospermia ◽  
Oocyte Activation ◽  
Control Groups ◽  
Birth Rates ◽  
Live Birth Rates ◽  
Significant Difference

Background: Failed oocyte activation following intracytoplasmic sperm injection (ICSI) as a result of calcium deficiency is a major challenge. Objective: We compared the effect of cult-active medium (CAM) on ICSI outcomes in obstructive azoospermia cases. Materials and Methods: The present study was conducted with 152 ICSI cases, classified into CAM and control groups. The injected oocytes in the control group were cultured in the cleavage medium, while in the artificial oocyte activation group, oocytes were chemically activated through exposure to 200 µL of CAM for 15 min. Fertilization and cleavage rates, quality of embryos, and biochemical pregnancy and live birth rates were assessed in both groups. Results: There were significant differences between the groups in terms of fertilization and cleavage rates after using the CAM in the percutaneous epididymal sperm aspiration (PESA) subgroup (p = 0.05, p ≤ 0.001) and in the testicular sperm extraction subgroup (p = 0.02, p = 0.04), compared to their control groups. Also, the pregnancy rate was significantly higher in the PESA-CAM subgroup (p = 0.03). The PESA-CAM subgroup demonstrated a significant difference in embryo quality after ICSI (p = 0.04). Unsuccessful embryo transfer and abortion were lower in both subgroups compared to the control groups, but this difference was not significant. Surprisingly, live birth rate was higher in the PESA-CAM subgroup (p = 0.03). Conclusion: CAM treatment could improve fertilization and cleavage rates in obstructive azoospermia participants. It had a significant effect on embryo quality, and pregnancy and live birth rates in PESA cases. Key words: Calcium ionophore, Obstructive azoospermia, Fertilization, ICSI.

Successful pregnancy following oocyte activation by strontium in normozoospermic patients of unexplained infertility with fertilisation failures during previous intracytoplasmic sperm injection treatment

2010 ◽  
Vol 22 (5) ◽  
pp. 852 ◽  
Author(s):  
Juan Chen ◽  
Yun Qian ◽  
Yong Tan ◽  
Hirofumi Mima
Keyword(s):  
Clinical Outcomes ◽  
Intracytoplasmic Sperm Injection ◽  
Embryo Quality ◽  
Unexplained Infertility ◽  
Oocyte Activation ◽  
High Quality ◽  
Successful Pregnancy ◽  
Efficient Treatment ◽  
Healthy Female ◽  
Assisted Oocyte Activation

Fertilisation failures occur in 2–3% of intracytoplasmic sperm injection (ICSI) cycles and are mostly caused by failure of oocyte activation. Assisted oocyte activation (AOA) may be an efficient treatment option to overcome oocyte activation failure. To evaluate the effectiveness of ICSI combined with AOA by strontium, six couples with complete fertilisation failure or low fertilisation rates (ranging from 0% to 16.7%; mean = 7.7%) in previous ICSI cycles were involved in the present study. In the latest ICSI cycles, AOA by strontium treatment was combined with ICSI to improve clinical outcomes. Fifty-two mature oocytes retrieved from six females were stimulated by strontium treatment after ICSI procedure, and 41 (78.8%) of them were successfully fertilised. The high-quality embryo rate was 41.5% (17/41) after culture for 5 days. Thirteen embryos were transferred (ranging from 2 to 3 per individual) resulting in three clinical pregnancies and three healthy babies were born. Furthermore, a pregnancy resulting in the birth of a healthy female infant was achieved following transfer of three frozen–thawed embryos. In conclusion, it appears that strontium treatment would be an effective method for AOA to improve fertilisation rates and embryo quality in cases with fertilisation failure after ICSI.

Successful pregnancy and childbirth after intracytoplasmic sperm injection with calcium ionophore oocyte activation in a globozoospermic patient

2008 ◽  
Vol 90 (4) ◽  
pp. 1202.e1-1202.e5 ◽  
Author(s):  
Alberto Tejera ◽  
Marta Mollá ◽  
Lourdes Muriel ◽  
Jose Remohí ◽  
Antonio Pellicer ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Calcium Ionophore ◽  
Oocyte Activation ◽  
Successful Pregnancy ◽  
Pregnancy And Childbirth

scholarly journals In vitro fertilisation (IVF) versus intracytoplasmic sperm injection (ICSI) in patients without severe male factor infertility: study protocol for the randomised, controlled, multicentre trial INVICSI

BMJ Open ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. e051058
Author(s):  
Sine Berntsen ◽  
Bugge Nøhr ◽  
Marie Louise Grøndahl ◽  
Morten Rønn Petersen ◽  
Lars Franch Andersen ◽  
...  
Keyword(s):  
Live Birth ◽  
Intracytoplasmic Sperm Injection ◽  
Controlled Trial ◽  
Male Partner ◽  
In Vitro Fertilisation ◽  
Male Factor Infertility ◽  
Male Factor ◽  
Fertilisation Rate ◽  
Randomised Controlled

IntroductionOver the last decades, the use of intracytoplasmic sperm injection (ICSI) has increased, even among patients without male factor infertility. The increase has happened even though there is no evidence to support that ICSI results in higher live birth rates compared with conventional in vitro fertilisation (IVF) in cases with nonmale factor infertility. The lack of robust evidence on an advantage of using ICSI over conventional IVF in these patients is problematic since ICSI is more invasive, complex and requires additional resources, time and effort. Therefore, the primary objective of the IVF versus ICSI (INVICSI) study is to determine whether ICSI is superior to standard IVF in patients without severe male factor infertility. The primary outcome measure is first live birth from fresh and frozen-thawed transfers after one stimulated cycle. Secondary outcomes include fertilisation rate, ongoing pregnancy rate, birth weight and congenital anomalies.Methods and analysisThis is a two-armed, multicentre, randomised, controlled trial. In total, 824 couples/women with infertility without severe male factor will be recruited and allocated randomly into two groups (IVF or ICSI) in a 1:1 ratio. Participants will be randomised in variable block sizes and stratified by trial site and age. The main inclusion criteria are (1) no prior IVF/ICSI treatment, (2) male partner sperm with an expected count of minimum 2 million progressive motile spermatozoa following density gradient purification on the day of oocyte pick up and (3) age of the woman between 18 and 42 years.Ethics and disseminationThe study will be performed in accordance with the ethical principles in the Helsinki Declaration. The study is approved by the Scientific Ethical Committee of the Capital Region of Denmark. Study findings will be presented, irrespectively of results at international conferences and submitted for publication in peer-reviewed journals.Trial registration numberNCT04128904. Pre-results.

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