Comet assay on mouse oocytes: an improved technique to evaluate genotoxic risk on female germ cells

2011 ◽  
Vol 95 (4) ◽  
pp. 1452-1457 ◽  
Author(s):  
Anais Berthelot-Ricou ◽  
Jeanne Perrin ◽  
Carole Di Giorgio ◽  
Michel De Meo ◽  
Alain Botta ◽  
...  
Keyword(s):  
Comet Assay ◽  
Germ Cells ◽  
Mouse Oocytes ◽  
Genotoxic Risk ◽  
Improved Technique

scholarly journals Mouse oocytes derived from fetal germ cells are competent to support the development of embryos by in vitro fertilization

2008 ◽  
Vol 75 (11) ◽  
pp. 1688-1689
Author(s):  
Wei Shen ◽  
Lan Li ◽  
Donghui Zhang ◽  
Qingjie Pan ◽  
Mingxiao Ding ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Germ Cells ◽  
Vitro Fertilization ◽  
Mouse Oocytes

Genotoxicity assessment of mouse oocytes by comet assay before vitrification and after warming with three vitrification protocols

2013 ◽  
Vol 100 (3) ◽  
pp. 882-888 ◽  
Author(s):  
Anais Berthelot-Ricou ◽  
Jeanne Perrin ◽  
Carole di Giorgio ◽  
Michel de Meo ◽  
Alain Botta ◽  
...  
Keyword(s):  
Comet Assay ◽  
Mouse Oocytes

scholarly journals Fertilization of mouse oocytes using somatic cells as male germ cells

2001 ◽  
Vol 3 (3) ◽  
pp. 205-211 ◽  
Author(s):  
Orly Lacham-Kaplan ◽  
Rob Daniels ◽  
Alan Trounson
Keyword(s):  
Germ Cells ◽  
Somatic Cells ◽  
Male Germ Cells ◽  
Mouse Oocytes

Biomonitoring of genotoxic risk in workers in a rubber factory: comparison of the Comet assay with cytogenetic methods and immunology

1999 ◽  
Vol 445 (2) ◽  
pp. 181-192 ◽  
Author(s):  
M Somorovská ◽  
E Szabová ◽  
P Vodička ◽  
J Tulinská ◽  
M Barančoková ◽  
...  
Keyword(s):  
Comet Assay ◽  
Genotoxic Risk ◽  
Cytogenetic Methods

Chapter 15. The Comet Assay in Sperm—Assessing Genotoxins in Male Germ Cells

2016 ◽  
pp. 390-456
Author(s):  
Adolf Baumgartner ◽  
Aftab Ali ◽  
Charmaine Normington ◽  
Diana Anderson
Keyword(s):  
Comet Assay ◽  
Germ Cells ◽  
Male Germ Cells

scholarly journals Growth of Mouse Oocytes to Maturity from Premeiotic Germ Cells In Vitro

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e41771 ◽  
Author(s):  
Zhi-Peng Zhang ◽  
Gui-Jin Liang ◽  
Xi-Feng Zhang ◽  
Guo-Liang Zhang ◽  
Hu-He Chao ◽  
...  
Keyword(s):  
Germ Cells ◽  
Mouse Oocytes

scholarly journals Genotoxic effect of restraint and stress pheromone on somatic and germ cells of mouse males Mus musculus L.

2021 ◽  
Vol 19 (2) ◽  
pp. 169-179
Author(s):  
Veronika D. Shcherbinina ◽  
Marina V. Petrova ◽  
Timofey S. Glinin ◽  
Eugene V. Daev
Keyword(s):  
Bone Marrow ◽  
Comet Assay ◽  
Germ Cells ◽  
Mus Musculus ◽  
Genotoxic Effect ◽  
Genome Integrity ◽  
Cell Frequency ◽  
Testicular Cells ◽  
First Time ◽  
In Cells

BACKGROUND: Different stressors affect the genome integrity, but the mechanisms of such action are underexplored. MATERIALS AND METHODS: Bone marrow and testicular cells of CBA and CD-1 mouse males were used to estimate their genome integrity after stressor action by the comet assay. RESULTS: It is shown here that restraint and 2,5-dimethylpyrazine both increase damaged cell frequency in bone marrow as well as in testes of mouse males. For the first time the effect of immobilization and 2,5-dimethylpyrazine in testicular cells is demonstrated using the comet assay. Both stressors have similar effects in cells of both tissues analyzed. CONCLUSION: Mechanisms of the effects and possible role in microevolution are under discussion.

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