scholarly journals Genotoxic effect of restraint and stress pheromone on somatic and germ cells of mouse males Mus musculus L.

2021 ◽  
Vol 19 (2) ◽  
pp. 169-179
Author(s):  
Veronika D. Shcherbinina ◽  
Marina V. Petrova ◽  
Timofey S. Glinin ◽  
Eugene V. Daev
Keyword(s):  
Bone Marrow ◽  
Comet Assay ◽  
Germ Cells ◽  
Mus Musculus ◽  
Genotoxic Effect ◽  
Genome Integrity ◽  
Cell Frequency ◽  
Testicular Cells ◽  
First Time ◽  
In Cells

BACKGROUND: Different stressors affect the genome integrity, but the mechanisms of such action are underexplored. MATERIALS AND METHODS: Bone marrow and testicular cells of CBA and CD-1 mouse males were used to estimate their genome integrity after stressor action by the comet assay. RESULTS: It is shown here that restraint and 2,5-dimethylpyrazine both increase damaged cell frequency in bone marrow as well as in testes of mouse males. For the first time the effect of immobilization and 2,5-dimethylpyrazine in testicular cells is demonstrated using the comet assay. Both stressors have similar effects in cells of both tissues analyzed. CONCLUSION: Mechanisms of the effects and possible role in microevolution are under discussion.

scholarly journals Genotoxic Effect of Silk Dyeing Wastes in Bone Marrow Cells of Mice, Mus musculus

CYTOLOGIA ◽  
2005 ◽  
Vol 70 (4) ◽  
pp. 381-384 ◽  
Author(s):  
Om Prakash Chaurasia ◽  
Alok Kumar ◽  
Moushmi Kumari
Keyword(s):  
Bone Marrow ◽  
Mus Musculus ◽  
Bone Marrow Cells ◽  
Genotoxic Effect ◽  
Silk Dyeing ◽  
Marrow Cells

Genotoxic Effect of Methotrexate on Bone Marrow Chromosomes and DNA of Male Albino Mice (Mus Musculus)

2016 ◽  
Vol 64 ◽  
pp. 350-363 ◽  
Author(s):  
Nagla Zaky Ibrahim El-Alfy ◽  
Mahmoud Fathy Mahmoud ◽  
Sally Ramadan Gabr El-Ashry
Keyword(s):  
Bone Marrow ◽  
Mus Musculus ◽  
Genotoxic Effect ◽  
Albino Mice

An investigation of bone marrow and testicular cells in vivo using the comet assay

1996 ◽  
Vol 370 (3-4) ◽  
pp. 159-174 ◽  
Author(s):  
Diana Anderson ◽  
Alok Dhawan ◽  
Tian-Wei Yu ◽  
Michael J. Plewa
Keyword(s):  
Bone Marrow ◽  
Comet Assay ◽  
Testicular Cells

Use of the comet assay to measure DNA damage in cells exposed to photosensitizers and gamma radiation

1999 ◽  
Vol 96 (1) ◽  
pp. 143-146 ◽  
Author(s):  
J.-P. Pouget ◽  
J.-L. Ravanat ◽  
T. Douki ◽  
M.-J. Richard ◽  
J. Cadet
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Gamma Radiation ◽  
In Cells

scholarly journals Curcumin activates Nrf2 through PKCδ-mediated p62 phosphorylation at Ser351

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jee-Yun Park ◽  
Hee-Young Sohn ◽  
Young Ho Koh ◽  
Chulman Jo
Keyword(s):  
Oxidative Stress ◽  
Crucial Role ◽  
Curcuma Longa ◽  
Expression Plasmid ◽  
Expression Of Genes ◽  
Nrf2 Activation ◽  
First Time ◽  
Nrf2 Protein ◽  
Endogenous Defense ◽  
In Cells

AbstractCurcumin, a phytochemical extracted from Curcuma longa rhizomes, is known to be protective in neurons via activation of Nrf2, a master regulator of endogenous defense against oxidative stress in cells. However, the exact mechanism by which curcumin activates Nrf2 remains controversial. Here, we observed that curcumin induced the expression of genes downstream of Nrf2 such as HO-1, NQO1, and GST-mu1 in neuronal cells, and increased the level of Nrf2 protein. Notably, the level of p62 phosphorylation at S351 (S349 in human) was significantly increased in cells treated with curcumin. Additionally, curcumin-induced Nrf2 activation was abrogated in p62 knockout (−/−) MEFs, indicating that p62 phosphorylation at S351 played a crucial role in curcumin-induced Nrf2 activation. Among the kinases involved in p62 phosphorylation at S351, PKCδ was activated in curcumin-treated cells. The phosphorylation of p62 at S351 was enhanced by transfection of PKCδ expression plasmid; in contrast, it was inhibited in cells treated with PKCδ-specific siRNA. Together, these results suggest that PKCδ is mainly involved in curcumin-induced p62 phosphorylation and Nrf2 activation. Accordingly, we demonstrate for the first time that curcumin activates Nrf2 through PKCδ-mediated p62 phosphorylation at S351.

scholarly journals Aberrant Expression of TLR2, TLR7, TLR9, Splicing Variants of TLR4 and MYD88 in Chronic Lymphocytic Leukemia Patients

2021 ◽  
Vol 10 (4) ◽  
pp. 867
Author(s):  
Katarzyna Skorka ◽  
Paulina Wlasiuk ◽  
Agnieszka Karczmarczyk ◽  
Krzysztof Giannopoulos
Keyword(s):  
Bone Marrow ◽  
Chronic Lymphocytic Leukemia ◽  
Cytotoxic T Cells ◽  
Lymphocytic Leukemia ◽  
Aberrant Expression ◽  
Downstream Signaling ◽  
Splicing Variants ◽  
High Level ◽  
Time To First Treatment ◽  
First Time

Functional toll-like receptors (TLRs) could modulate anti-tumor effects by activating inflammatory cytokines and the cytotoxic T-cells response. However, excessive TLR expression could promote tumor progression, since TLR-induced inflammation might stimulate cancer cells expansion into the microenvironment. Myd88 is involved in activation NF-κB through TLRs downstream signaling, hence in the current study we provided, for the first time, a complex characterization of expression of TLR2, TLR4, TLR7, TLR9, and MYD88 as well as their splicing forms in two distinct compartments of the microenvironment of chronic lymphocytic leukemia (CLL): peripheral blood and bone marrow. We found correlations between MYD88 and TLRs expressions in both compartments, indicating their relevant cooperation in CLL. The MYD88 expression was higher in CLL patients compared to healthy volunteers (HVs) (0.1780 vs. 0.128, p < 0.0001). The TLRs expression was aberrant in CLL compared to HVs. Analysis of survival curves revealed a shorter time to first treatment in the group of patients with low level of TLR4(3) expression compared to high level of TLR4(3) expression in bone marrow (13 months vs. 48 months, p = 0.0207). We suggest that TLRs expression is differentially regulated in CLL but is similarly shared between two distinct compartments of the microenvironment.

scholarly journals Analysis of DNA damage in cells excreted in urine of cervical cancer patients using alkaline comet assay

2014 ◽  
Vol 7 (Suppl 1) ◽  
pp. P12
Author(s):  
Avani Patel ◽  
Mihir Shah ◽  
Pinaki Patel ◽  
Trupti Patel
Keyword(s):  
Cervical Cancer ◽  
Dna Damage ◽  
Comet Assay ◽  
Cancer Patients ◽  
Alkaline Comet Assay ◽  
In Cells
Sign in / Sign up

Export Citation Format

Share Document