Oocyte ageing is associated with altered metabolic stress response and lower mitochondrial DNA copy number that correlate with intracellular NADH and FAD measured by Fluorescence Lifetime Imaging Microscopy (FLIM)

2016 ◽  
Vol 106 (3) ◽  
pp. e69 ◽  
Author(s):  
E. Babayev ◽  
T. Wang ◽  
T. Sanchez ◽  
K. Lowther ◽  
H.S. Taylor ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Stress Response ◽  
Fluorescence Lifetime ◽  
Copy Number ◽  
Metabolic Stress ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Dna Copy Number ◽  
Lifetime Imaging ◽  
Mitochondrial Dna Copy Number

scholarly journals Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

2016 ◽  
Vol 52 (24) ◽  
pp. 4458-4461 ◽  
Author(s):  
Hideki Itoh ◽  
Satoshi Arai ◽  
Thankiah Sudhaharan ◽  
Sung-Chan Lee ◽  
Young-Tae Chang ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Heat Production ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
C2c12 Myotube

FLIM of ER thermo yellow and non-targeted mCherry reveals the Ca2+-dependent heat production localized to SR in C2C12 myotube.

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