A predictive fertility treatment model based on oocyte quality and reactive oxygen species

Copper (Cu) is an essential trace element for animals, and also an important nutritional component for the normal physiology and metabolism of animal reproductive systems. An excess or lack of Cu will directly or indirectly affect animal reproductive activities. However, the effect of Cu, in particular excessive Cu, on the reproductive performance of sows has not been studied. Here, we report that excessive Cu had negative effects on oocyte maturation and organelle functions. We showed that Cu exposure perturbed porcine oocyte meiotic maturation and impaired spindle/chromosome structure, resulting in a defective spindle assembly, as well as the abnormal distribution of actin dynamics and cortical granules. In addition, single-cell transcriptome analysis identified the target effectors of Cu actions in porcine oocytes, further demonstrating that Cu exposure affects the mitochondrial distribution and function, leading to the high levels of reactive oxygen species, DNA damage, and early apoptosis of porcine oocytes. These findings demonstrate that Cu exposure causes abnormalities in the mitochondrial distribution and function, resulting in the increased oxidative stress and levels of reactive oxygen species, DNA damage, and apoptosis, ultimately leading to a decreased porcine oocyte quality.

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270 MITOCHONDRIA AND REACTIVE OXYGEN SPECIES COLOCALIZATION IN IN VIVO AND IN VITRO MATURED OOCYTES FROM SUPEROVULATED ADULT EWES

Reproduction Fertility and Development ◽

10.1071/rdv23n1ab270 ◽

2011 ◽

Vol 23 (1) ◽

pp. 233

Author(s):

B. Ambruosi ◽

N. A. Martino ◽

M. Filioli Uranio ◽

F. Silvestre ◽

F. Binetti ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Cumulus Cell ◽

Reactive Oxygen ◽

Oocyte Quality ◽

Nuclear Chromatin ◽

Oxygen Species ◽

Fluorescent Intensity ◽

Intracellular Ros

Analyses of energy and redox status parameters are emerging technologies to improve oocyte quality assessment. Mitochondria (mt) play a vital role in the oocyte to support maturation, fertilization, and pre-implantation development. They are the major source of reactive oxygen species (ROS) produced during oxidative phosphorylation, which are not only by-products of cell metabolism but also important molecules for regulation of intracellular cell signaling. The aim of the present study was to test for mt/ROS colocalization in oocytes recovered from superovulated adult ewes and examined after in vivo or in vitro maturation (IVM). Cumulus–oocyte complexes of 8 superovulated (fluorogestone acetate + D-cloprostenol for oestrus synchronization, pFSH/pLH and eCG for superovulation) adult (2 to 8 years of age) ewes were recovered (ovariohysterectomy by midventral laparotomy performed 54 h after vaginal sponge removal) either from flushing oviducts (oviducal oocytes) or from ovarian growing follicles (1–5 mm in diameter; follicular oocytes). Follicular oocytes were analysed after IVM (Ambruosi et al. 2009 Theriogenology 71, 1093–1104). After cumulus cell removal, all oocytes underwent nuclear chromatin, mt, and ROS evaluation. Hoechst 33258 and Mitotracker Orange CMTM Ros were used to label nuclear chromatin and mt (Ambruosi et al. 2009) and 2′,7′-dichloro-dihydro-fluorescein diacetate was used for ROS labelling (Hashimoto et al. 2000 Mol. Reprod. Dev. 57, 353–360). Oocytes at the metaphase II (MII) stage showing regular ooplasmic size (>130 μm in diameter) and morphology were selected for confocal analysis of mt/ROS fluorescence distribution, intensity, and colocalization. Forty oviducal MII oocytes recovered from 8 ewes were analysed. Thirty-two oocytes recovered from the ovaries of 4 ewes underwent IVM, and 23 out of 32 (72%) reached nuclear maturation and were analysed. The rate of oocytes showing perinuclear mt distribution pattern did not differ between oviducal and IVM oocytes (33%, 13/40 v. 43%, 10/23; not significant). In these oocytes, fluorescent intensity of mt labelling and intracellular ROS levels did not differ between oviducal and IVM ooocytes (996.27 ± 363.57 v. 798.13 ± 275.91; not significant; and 1808.11 ± 442.78 v. 1473.29 ± 662.49, for mt and ROS, respectively; not significant), whereas mt/ROS colocalization was significantly higher in ovulated oocytes than in IVM oocytes (Pearson coefficient 0.67 ± 0.11 v. 0.39 ± 0.19, respectively; P < 0.001). In conclusion, in oocytes of adult ewes, mt aggregation, apparent energy status, and intracellular ROS levels do not differ between ovulated and IVM oocytes, but mt/ROS colocalization differs between the 2 groups. As it was reported for other cell systems that such a difference can be indicative of healthy status of ovulated oocytes, we suggest that mt/ROS colocalization could be considered as a suitable marker of oocyte quality. Financial support was provided by Fondazione Cassa di Risparmio di Puglia 2008. Project: Salvaguardia di razze ovine autoctone pugliesi (R.U. DPA Resp. Sci. Prof. M. E. DellAquila).

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Implications of enzyme deficiencies on mitochondrial energy metabolism and reactive oxygen species formation of neurons involved in rotenone-induced Parkinson's disease: a model-based analysis

FEBS Journal ◽

10.1111/febs.12480 ◽

2013 ◽

Vol 280 (20) ◽

pp. 5080-5093 ◽

Cited By ~ 14

Author(s):

Nikolaus Berndt ◽

Herrmann-Georg Holzhütter ◽

Sascha Bulik

Keyword(s):

Reactive Oxygen Species ◽

Parkinson’S Disease ◽

Energy Metabolism ◽

Reactive Oxygen ◽

Oxygen Species ◽

Reactive Oxygen Species Formation ◽

Mitochondrial Energy Metabolism ◽

Model Based ◽

Species Formation ◽

Model Based Analysis

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Assessment of oocyte quality in polycystic ovarian syndrome and endometriosis by spindle imaging and reactive oxygen species levels in follicular fluid and its relationship with IVF-ET outcome

Journal of Human Reproductive Sciences ◽

10.4103/0974-1208.101020 ◽

2012 ◽

Vol 5 (2) ◽

pp. 187 ◽

Cited By ~ 27

Author(s):

Shweta Rajani ◽

Sunita Sharma ◽

Baidyanath Chakravarty ◽

Ratna Chattopadhyay ◽

Sanghamitra Ghosh ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Follicular Fluid ◽

Polycystic Ovarian Syndrome ◽

Reactive Oxygen ◽

Oocyte Quality ◽

Oxygen Species ◽

Ovarian Syndrome ◽

Ivf Et

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Suplementasi Antioksidan pada Maturasi In Vitro Menurunkan Kadar ROS: A Literature Review

Jurnal Penelitian Kesehatan SUARA FORIKES (Journal of Health Research Forikes Voice) ◽

10.33846/sf11105 ◽

2019 ◽

Vol 11 (1) ◽

pp. 23

Author(s):

Amalia Ratna Kusumaningrum

Keyword(s):

Reactive Oxygen Species ◽

Literature Review ◽

Assisted Reproductive Technology ◽

In Vitro Maturation ◽

Reactive Oxygen ◽

Reproductive Technology ◽

Fertility Treatment ◽

Oxygen Species ◽

Survival And Development

One of the reproductive health problems is infertility. In vitro maturation (IVM) is emerging as a popular technology at the forefront of fertility treatment. IVM conditions inducing a series of events related to reactive oxygen species (ROS), which have been implicated as one of the major causes for reduced embryonic development. Therefore, the supplementation of IVM media with antioxidants could improve the survival and development of the oocytes. The method of searching conducted online database such as Science Direct, Sage, Pubmed, Springer, and Oxford. The keywords that used were “antioxidant”, “reactive oxygen species”, “in vitro maturation”, “infertility” and “assisted reproductive technology”. The literatures restricted in 2008 year until 2019 in English. The results of the study show that there were 3 main themes, types, dosages, antioxidant benefits for reduced ROS levels during IVM. The drawback that might arise in this study is that there are still possibilities of not getting some related articles. The literature review is expected to be a basis for research assisted reproductive technology. Keywords: antioxidant; in vitro maturation; infertility; Reactive oxygen species (ROS)

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Mitochondria as a source of reactive oxygen species

Biochemical Journal ◽

10.1042/bj20090056 ◽

2009 ◽

pp. c3 ◽

Cited By ~ 1

Author(s):

Helena M. Cochemé ◽

Michael P. Murphy

Keyword(s):

Reactive Oxygen Species ◽

Reactive Oxygen ◽

Oxygen Species

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Clinical aspects of reactive oxygen and nitrogen species

Biochemical Society Symposium ◽

10.1042/bss0710121 ◽

2004 ◽

Vol 71 ◽

pp. 121-133 ◽

Cited By ~ 25

Author(s):

Ascan Warnholtz ◽

Maria Wendt ◽

Michael August ◽

Thomas Münzel

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Risk Factor ◽

Endothelial Dysfunction ◽

Vascular Tissue ◽

Rapid Development ◽

Reactive Oxygen ◽

Clinical Aspects ◽

No Production ◽

Oxygen Species

Endothelial dysfunction in the setting of cardiovascular risk factors, such as hypercholesterolaemia, hypertension, diabetes mellitus and chronic smoking, as well as in the setting of heart failure, has been shown to be at least partly dependent on the production of reactive oxygen species in endothelial and/or smooth muscle cells and the adventitia, and the subsequent decrease in vascular bioavailability of NO. Superoxide-producing enzymes involved in increased oxidative stress within vascular tissue include NAD(P)H-oxidase, xanthine oxidase and endothelial nitric oxide synthase in an uncoupled state. Recent studies indicate that endothelial dysfunction of peripheral and coronary resistance and conductance vessels represents a strong and independent risk factor for future cardiovascular events. Ways to reduce endothelial dysfunction include risk-factor modification and treatment with substances that have been shown to reduce oxidative stress and, simultaneously, to stimulate endothelial NO production, such as inhibitors of angiotensin-converting enzyme or the statins. In contrast, in conditions where increased production of reactive oxygen species, such as superoxide, in vascular tissue is established, treatment with NO, e.g. via administration of nitroglycerin, results in a rapid development of endothelial dysfunction, which may worsen the prognosis in patients with established coronary artery disease.

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