scholarly journals Bisphenol a (BPA) levels in the serum of indian women collected at the time of oocyte retrieval may predict BPA levels in their follicular fluid (FF)

2018 ◽  
Vol 110 (4) ◽  
pp. e171
Author(s):  
F.R. Parikh ◽  
S. Uttamchandani ◽  
A. Athalye ◽  
P. Sinkar ◽  
A.S. Velumani ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Follicular Fluid ◽  
Oocyte Retrieval ◽  
Indian Women

scholarly journals Estradiol and leptin as conditional prognostic IVF markers

Reproduction ◽  
2005 ◽  
Vol 129 (4) ◽  
pp. 531-534 ◽  
Author(s):  
G Anifandis ◽  
E Koutselini ◽  
K Louridas ◽  
V Liakopoulos ◽  
K Leivaditis ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Embryo Quality ◽  
Oocyte Retrieval ◽  
Dependent Manner ◽  
Ivf Outcome ◽  
Concentration Dependent Manner ◽  
Conditional Interaction ◽  
Estradiol Levels ◽  
Ivf Et

We studied the concentration of serum estradiol and serum and follicular fluid leptin in 200 women undergoing their first in vitro fertilization with embryo transfer (IVF-ET) program at the time of human chorionic gonadotrophin administration and oocyte retrieval, in an attempt to assess their concerted role on embryo quality and the prognosis of IVF outcome. Low serum (46.49 ± 8.4 ng/ml) and follicular fluid (52 ± 9.8 ng/ml) leptin levels were associated with a high number of ‘good-quality’ embryos (73.6%) and high implantation (11.2%) and pregnancy (35.8%) rates and were observed in women with normal peak estradiol levels of between 1000 and 2000 pg/ml. It appears that leptin and estradiol interact coordinately in a concentration-dependent manner to control IVF outcome. Further studies will be required to substantiate and clarify the mechanism of proposed conditional interaction between the two hormonal systems.

scholarly journals Propofol concentrations in follicular fluid during general anaesthesia for transvaginal oocyte retrieval

1999 ◽  
Vol 14 (2) ◽  
pp. 345-348 ◽  
Author(s):  
F. Christiaens ◽  
C. Janssenswillen ◽  
C. Verborgh ◽  
I. Moerman ◽  
P. Devroey ◽  
...  
Keyword(s):  
General Anaesthesia ◽  
Follicular Fluid ◽  
Oocyte Retrieval ◽  
Transvaginal Oocyte Retrieval

Remifentanil and Fentanyl Concentrations in Follicular Fluid During Transvaginal Oocyte Retrieval

1999 ◽  
Vol 90 (Supplement) ◽  
pp. 16A ◽  
Author(s):  
Mohan Achwal ◽  
Mostafa Abuzeid ◽  
Jody L. Bovenschen ◽  
Keith A. Lawrence ◽  
Harland Verrill ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Oocyte Retrieval ◽  
Transvaginal Oocyte Retrieval

132 Influence of oocyte retrieval methods and maturation media on invitro development of polar body extrusion in pig oocytes

2021 ◽  
Vol 33 (2) ◽  
pp. 174
Author(s):  
K. M. Honneysett ◽  
M. L. Mphaphathi ◽  
A. M. Maqhashu ◽  
E. C. Webb
Keyword(s):  
Follicular Fluid ◽  
Cell Layer ◽  
Polar Body ◽  
Cumulus Cell ◽  
Oocyte Retrieval ◽  
Cumulus Cells ◽  
Reproductive Technology ◽  
Maturation Stage ◽  
Significant Difference ◽  
Polar Body Extrusion

Oocyte recovery is a reproductive technology that can be done by using two techniques, aspiration and slicing. Invitro maturation (IVM) is an additional reproductive technology used to advance an oocyte to a maturation stage; thereafter, it may be used during IVF. The objectives of the present study were (1) to compare two different oocyte retrieval methods (aspiration and slicing) from pig ovaries on oocyte quality and quantity, and (2) to compare three different IVM media [NCSU 37, TCM-199, and modified porcine follicular fluid (mpFF=porcine follicular fluid+FSH+LH] on oocytes’ polar body extrusion. During aspiration, an 18G needle was attached to a 10-mL syringe and all visible follicles were aspirated. During slicing, a surgical blade was used to slice the ovaries held in mDPBS. Follicular fluid collected from both methods was assessed for the presence of oocytes with the aid of a microscope. The collected oocytes were then categorized as Grade A, B, or C: Grade A=oocytes with compacted, multilayered cumulus cells and a homogeneous ooplasm; Grade B=oocytes with a compact cumulus cell layer with homogeneous ooplasm; Grade C=oocytes with a less compact cumulus cell layer with irregular ooplasm containing dark granules. The IVM media were placed in a four-well multidish; thereafter Grades A and B oocytes were allocated per treatment groups and matured for 44h. The treatment means were compared using the Fisher’s protected t-test least significant difference. The results showed significant differences between the grades of oocytes (P<0.05) with Grade A and B oocytes accounting for 50.8% of total oocytes (193.8) for aspiration and 58.7% of total oocytes (488.6) for slicing. The oocytes polar body extrusion was recorded as 25.3, 84.2, and 73.8% for NCSU 37 (P<0.05) and TCM-199 and mpFF respectively (P>0.05). In conclusion, the slicing method proved to be better than aspiration with regards to the retrieval of Grades A and B oocytes as well as the total number of oocytes retrieved. The TCM-199 and mpFF media had a higher percentage of oocytes with polar body extrusion than NCSU 37.

scholarly journals miR-15a-5p levels correlate with poor ovarian response in human follicular fluid

Reproduction ◽  
2017 ◽  
Vol 154 (4) ◽  
pp. 483-496 ◽  
Author(s):  
Kaiyue Zhang ◽  
Wanxia Zhong ◽  
Wei-Ping Li ◽  
Zi-Jiang Chen ◽  
Cong Zhang
Keyword(s):  
Follicular Fluid ◽  
Quantitative Polymerase Chain Reaction ◽  
Young Patients ◽  
Oocyte Retrieval ◽  
Ovarian Response ◽  
Poor Ovarian Response ◽  
Human Follicular Fluid ◽  
Transfer Procedures ◽  
Response Group

Poor ovarian response is a significant problem encountered during in vitro fertilization and embryo transfer procedures. Many infertile women may suffer from poor ovarian response and its incidence tends to be increasing in young patients nowadays. It is a major cause of maternal infertility because it is associated with low pregnancy and live birth rates. However, the cause of poor ovarian response is not clear. In this study, we extracted microRNAs from human follicular fluid and performed miRNA sequencing to investigate a potential posttranscriptional mechanism underlying poor ovarian response. The results showed that many miRNAs were obviously different between the poor ovarian response and non-poor ovarian response groups. We then performed quantitative polymerase chain reaction, Western blot analysis and used an in vitro culture system to verify the sequencing results and to study the mechanism. Notably, we found that miRNA-15a-5p was significantly elevated in the young poor ovarian response group. Furthermore, we demonstrated that high levels of miR-15a-5p in the young poor ovarian response group repressed granulosa cell proliferation by regulating the PI3K-AKT-mTOR signaling pathway and promoted apoptosis through BCL2 and BAD. This could explain the reduced oocyte retrieval number seen in poor ovarian response patients.

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