SINGLE CELL TRANSCRIPTOME ANALYSIS IDENTIFIES PUTATIVE CELL SURFACE MARKERS OF HUMAN SPERMATOGONIAL STEM CELLS

Ongoing progress in genomic technologies offers exciting tools that can help to resolve transcriptome and genome-wide DNA modifications at single-cell resolution. These methods can be used to characterize individual cells within complex tissue organizations and to highlight various molecular interactions. Here, we will discuss recent advances in the definition of spermatogonial stem cells (SSC) and their progenitors in humans using the single-cell transcriptome sequencing (scRNAseq) approach. Exploration of gene expression patterns allows one to investigate stem cell heterogeneity. It leads to tracing the spermatogenic developmental process and its underlying biology, which is highly influenced by the microenvironment. scRNAseq already represents a new diagnostic tool for the personalized investigation of male infertility. One may hope that a better understanding of SSC biology could facilitate the use of these cells in the context of fertility preservation of prepubertal children, as a key component of regenerative medicine.

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Single-Cell Transcriptome Analysis of Neural Stem Cells

Current Pharmacology Reports ◽

10.1007/s40495-017-0084-3 ◽

2017 ◽

Vol 3 (2) ◽

pp. 68-76

Author(s):

Ying Li ◽

Jeremy Anderson ◽

Kelvin Y. Kwan ◽

Li Cai

Keyword(s):

Stem Cells ◽

Neural Stem Cells ◽

Single Cell ◽

Transcriptome Analysis ◽

Cell Transcriptome ◽

Single Cell Transcriptome

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Faculty Opinions recommendation of The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.734372554.793555701 ◽

2019 ◽

Author(s):

Miles Wilkinson

Keyword(s):

Stem Cells ◽

Single Cell ◽

Spermatogonial Stem Cells ◽

Cell Transcriptome ◽

Single Cell Transcriptome

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The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids

Cell Reports ◽

10.1016/j.celrep.2018.10.026 ◽

2018 ◽

Vol 25 (6) ◽

pp. 1650-1667.e8 ◽

Cited By ~ 105

Author(s):

Brian P. Hermann ◽

Keren Cheng ◽

Anukriti Singh ◽

Lorena Roa-De La Cruz ◽

Kazadi N. Mutoji ◽

...

Keyword(s):

Stem Cells ◽

Single Cell ◽

Spermatogonial Stem Cells ◽

Cell Transcriptome ◽

Single Cell Transcriptome

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Age-associated changes in the transcriptomes of non-cultured adipose-derived stem cells from young and old mice assessed via single-cell transcriptome analysis

PLoS ONE ◽

10.1371/journal.pone.0242171 ◽

2020 ◽

Vol 15 (11) ◽

pp. e0242171

Author(s):

Yuta Doshida ◽

Haruka Sano ◽

Sadahiro Iwabuchi ◽

Toshiro Aigaki ◽

Masayuki Yoshida ◽

...

Keyword(s):

Stem Cells ◽

Single Cell ◽

Transcriptome Analysis ◽

Adipose Derived Stem Cells ◽

High Gene Expression ◽

High Gene ◽

Differentiation Stage ◽

Cell Transcriptome ◽

Single Cell Transcriptome

Adipose-derived stem cells (ASCs) exhibit self-renewal and pluripotency. The differentiation potency of ASCs has been reported to deteriorate with aging; however, relevant studies used ASCs that were isolated and subcultured several times. It is still unclear whether subcultured ASCs accurately reflect the in vivo state. To address this question, we used freshly isolated stromal vascular fractions (SVFs) and performed comprehensive single-cell transcriptome analysis. In this study, we identified three cell populations as putative ASC candidates in SVFs and three novel ASC-related genes: Adamts7, Snai2, and Tgfbr1, that are reported to be negative regulators of cell differentiation. Moreover, we identified age-associated high gene expression levels of Adamts7, Egfr, and Igfbp4 in the earliest differentiation stage of ASCs. These results suggest that aging may make it impossible to maintain the stringency of the regulation of the expression of some genes related to ASC differentiation.

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