scholarly journals Contribution of Single-Cell Transcriptomics to the Characterization of Human Spermatogonial Stem Cells: Toward an Application in Male Fertility Regenerative Medicine?

2019 ◽  
Vol 20 (22) ◽  
pp. 5773 ◽  
Author(s):  
Anne-Sophie Gille ◽  
Clémentine Lapoujade ◽  
Jean-Philippe Wolf ◽  
Pierre Fouchet ◽  
Virginie Barraud-Lange
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Single Cell ◽  
Developmental Process ◽  
Expression Patterns ◽  
Spermatogonial Stem Cells ◽  
Genomic Technologies ◽  
Cell Transcriptome ◽  
Definition Of ◽  
Single Cell Transcriptome

Ongoing progress in genomic technologies offers exciting tools that can help to resolve transcriptome and genome-wide DNA modifications at single-cell resolution. These methods can be used to characterize individual cells within complex tissue organizations and to highlight various molecular interactions. Here, we will discuss recent advances in the definition of spermatogonial stem cells (SSC) and their progenitors in humans using the single-cell transcriptome sequencing (scRNAseq) approach. Exploration of gene expression patterns allows one to investigate stem cell heterogeneity. It leads to tracing the spermatogenic developmental process and its underlying biology, which is highly influenced by the microenvironment. scRNAseq already represents a new diagnostic tool for the personalized investigation of male infertility. One may hope that a better understanding of SSC biology could facilitate the use of these cells in the context of fertility preservation of prepubertal children, as a key component of regenerative medicine.

scholarly journals SINGLE CELL TRANSCRIPTOME ANALYSIS IDENTIFIES PUTATIVE CELL SURFACE MARKERS OF HUMAN SPERMATOGONIAL STEM CELLS

2020 ◽  
Vol 114 (3) ◽  
pp. e100-e101
Author(s):  
Sarah Munyoki ◽  
Adrienne N. Shami ◽  
Xianing Zheng ◽  
Qianyi Ma ◽  
Meena Sukhwani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Single Cell ◽  
Transcriptome Analysis ◽  
Spermatogonial Stem Cells ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

scholarly journals The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids

Cell Reports ◽  
2018 ◽  
Vol 25 (6) ◽  
pp. 1650-1667.e8 ◽  
Author(s):  
Brian P. Hermann ◽  
Keren Cheng ◽  
Anukriti Singh ◽  
Lorena Roa-De La Cruz ◽  
Kazadi N. Mutoji ◽  
...  
Keyword(s):  
Stem Cells ◽  
Single Cell ◽  
Spermatogonial Stem Cells ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

scholarly journals Single-Cell Transcriptome Analyses Reveal Signals to Activate Dormant Neural Stem Cells

Cell ◽  
2015 ◽  
Vol 161 (5) ◽  
pp. 1175-1186 ◽  
Author(s):  
Yuping Luo ◽  
Volkan Coskun ◽  
Aibing Liang ◽  
Juehua Yu ◽  
Liming Cheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Single Cell ◽  
Transcriptome Analyses ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

scholarly journals Single-cell transcriptome analysis of embryonic and adult endothelial cells allows to rank the hemogenic potential of post-natal endothelium

2021 ◽  
Author(s):  
Artem Adamov ◽  
Yasmin Natalia Serina Sechanecia ◽  
Christophe Lancrin
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Hematopoietic Stem Cells ◽  
Single Cell ◽  
Rational Design ◽  
Continuous Production ◽  
Cellular Reprogramming ◽  
Hematopoietic Stem ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

Hematopoietic stem cells are crucial for the continuous production of blood cells during life. The transplantation of these cells is one of the most common treatments to cure patient suffering of blood diseases. However, the lack of suitable donors is a major limitation. One option to get hematopoietic stem cells matching perfectly a patient is cellular reprogramming. Hematopoietic stem cells emerge from endothelial cells in blood vessels during embryogenesis through the endothelial to hematopoietic transition. Here, we used single-cell transcriptomics analysis to compare embryonic and post-natal endothelial cells to investigate the potential of adult vasculature to be reprogrammed in hematopoietic stem cells. Although transcriptional similarities have been found between embryonic and adult endothelial cells, we found some key differences in term of transcription factors expression. There is a deficit of expression of Runx1, Tal1, Lyl1 and Cbfb in adult endothelial cells compared to their embryonic counterparts. Using a combination of gene expression profiling and gene regulatory network analysis, we found that endothelial cells from the pancreas, brain, kidney and liver appear to be the most suitable targets for cellular reprogramming into hematopoietic stem cells. Overall, our work provides an important resource for the rational design of a reprogramming strategy for the generation of hematopoietic stem cells.

scholarly journals Single-Cell Transcriptome Analysis of Human Adipose-Derived Stromal Cells Identifies a Contractile Cell Subpopulation

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Elize Wolmarans ◽  
Juanita Mellet ◽  
Chrisna Durandt ◽  
Fourie Joubert ◽  
Michael S. Pepper
Keyword(s):  
Single Cell ◽  
Transcriptome Analysis ◽  
Stromal Cells ◽  
Exploratory Study ◽  
Expression Patterns ◽  
Cell Subpopulation ◽  
Adipose Derived Stromal Cells ◽  
Cell Transcriptome ◽  
Population Doublings ◽  
Single Cell Transcriptome

The potential for human adipose-derived stromal cells (hASCs) to be used as a therapeutic product is being assessed in multiple clinical trials. However, much is still to be learned about these cells before they can be used with confidence in the clinical setting. An inherent characteristic of hASCs that is not well understood is their heterogeneity. The aim of this exploratory study was to characterize the heterogeneity of freshly isolated hASCs after two population doublings (P2) using single-cell transcriptome analysis. A minimum of two subpopulations were identified at P2. A major subpopulation was identified as contractile cells which, based on gene expression patterns, are likely to be pericytes and/or vascular smooth muscle cells (vSMCs).

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