Genes associated with lignin degradation in the polyphagous white-rot pathogen Heterobasidion irregulare show substrate-specific regulation

AbstractOzonized Japanese cedar wood meal was evaluated as a feedstock for compost. The composting experiment performed in a 1.8 m3tank during a 4-week period showed that the decomposition of organics was accelerated by the ozonation of wood meal during thermophilic phase. The same is true for decay test of white-rot (WR) fungus. The tested brown-rot (BR) fungus did not show any effect. Accordingly, the lignin degradation by ozone is advantageous for composting. In addition, liberation of ammonia, one source of odor development, was suppressed during the thermophilic phase of composting of ozonized wood meal.

Download Full-text

Novel heme-containing enzyme possibly involved in lignin degradation by the white-rot fungusJunghuhnia separabilima

FEMS Microbiology Letters ◽

10.1111/j.1574-6968.1992.tb05541.x ◽

1992 ◽

Vol 99 (1) ◽

pp. 53-58 ◽

Cited By ~ 22

Author(s):

Tamara Vares ◽

Taina K. Lundell ◽

Annele I. Hatakka

Keyword(s):

Lignin Degradation ◽

White Rot

Download Full-text

Mn2+ Alters Peroxidase Profiles and Lignin Degradation by the White-Rot Fungus Pleurotus ostreatus Under Different Nutritional and Growth Conditions

Applied Biochemistry and Biotechnology ◽

10.1385/abab:102-103:1-6:415 ◽

2002 ◽

Vol 102-103 (1-6) ◽

pp. 415-430 ◽

Cited By ~ 20

Author(s):

Roni Cohen ◽

Limor Persky ◽

Zahit Hazan-Eitan ◽

Oded Yarden ◽

Yitzhak Hadar

Keyword(s):

Pleurotus Ostreatus ◽

Lignin Degradation ◽

Growth Conditions ◽

White Rot ◽

White Rot Fungus

Download Full-text

Enhancement of lignin degradation and laccase activity in Pleurotus ostreatus by cotton stalk extract

Canadian Journal of Microbiology ◽

10.1139/w98-054 ◽

1998 ◽

Vol 44 (7) ◽

pp. 676-680 ◽

Cited By ~ 17

Author(s):

Orly Ardon ◽

Zohar Kerem ◽

Yitzhak Hadar

Keyword(s):

Oxygen Consumption ◽

Pleurotus Ostreatus ◽

Lignin Degradation ◽

Laccase Activity ◽

White Rot ◽

White Rot Fungus ◽

Lignin Biodegradation ◽

Cotton Stalk ◽

Fermentation System ◽

Uv Visible

The white rot fungus Pleurotus ostreatus was grown in a chemically defined solid state fermentation system amended with cotton stalk extract (CSE).Treated cultures exhibited increased laccase activity as well as enhanced lignin mineralization. Mineralization of [14C]lignin initialized 4 days earlier in CSE-supplemented cultures than in control cultures. Total mineralization in the first 16 days was 15% in the CSE-treated cultures, compared with only 7% in the controls. Cotton stalk extract also contained compounds that serve as substrates for laccase purified from P. ostreatus as shown by oxygen consumption, as well as changes in the UVvisible spectrum.Key words: cotton, Pleurotusostreatus, white rot, laccase, lignin biodegradation.

Download Full-text

Lignin degrading system of white-rot fungi and its exploitation for dye decolorization

Canadian Journal of Microbiology ◽

10.1139/w02-090 ◽

2002 ◽

Vol 48 (10) ◽

pp. 857-870 ◽

Cited By ~ 56

Author(s):

Vishal Shah ◽

Frantisek Nerud

Keyword(s):

Lignin Degradation ◽

White Rot Fungi ◽

Alcohol Oxidase ◽

Dye Decolorization ◽

White Rot ◽

Low Molecular Weight ◽

Low Molecular Weight Compounds ◽

Pyranose Oxidase ◽

Degrading System ◽

Glyoxal Oxidase

With global attention and research now focused on looking for the abatement of pollution, white-rot fungi is one of the hopes of the future. The lignin-degrading ability of these fungi have been the focus of attention for many years and have been exploited for a wide array of human benefits. This review highlights the various enzymes produced by white-rot fungi for lignin degradation, namely laccases, peroxidases, aryl alcohol oxidase, glyoxal oxidase, and pyranose oxidase. Also discussed are the various radicals and low molecular weight compounds that are being produced by white-rot fungi and its role in lignin degradation. A brief summary on the developments in research of decolorization of dyes using white-rot fungi has been made.Key words: lignin degradation, white-rot fungi, laccase, peroxidase, radicals, dye decolorization.

Download Full-text

Biological pretreatment of wheat straw: Effect of fungal culturing on enzymatic hydrolysis of carbohydrate polymers

10.21203/rs.3.rs-227697/v1 ◽

2021 ◽

Author(s):

Aleksandar Knežević ◽

Ivana Đokić ◽

Tomislav Tosti ◽

Slađana Popović ◽

Dušanka Milojković-Opsenica ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Wheat Straw ◽

Lignin Degradation ◽

Enzymatic Saccharification ◽

White Rot Fungi ◽

Fungal Species ◽

White Rot ◽

Irpex Lacteus ◽

Fungal Pretreatment ◽

Hydrolysis Of

Abstract The aim of the study was comparative analysis of degradation of wheat straw lignin by white-rot fungi and its implications on the efficiency of enzymatic hydrolysis of holocellulose. Cyclocybe cylindracea, Ganoderma resinaceum, Irpex lacteus, Pleurotus ostreatus and Trametes versicolor were the species studied. Peroxidases were predominantly responsible for lignin degradation even though high laccase activities were detected, except in the case of Irpex lacteus where laccase activity was not detected. Studied fungal species showed various ability to degrade lignin in wheat straw which further affected release of reducing sugars during enzymatic saccharification. The highest rate of lignin degradation was noticed in sample pretreated with Irpex lacteus (50.9 ± 4.1%). Among all tested species only Ganoderma resinaceum was suitable lignin degrader with the 2-fold higher hydrolysis yield (51.1 ± 4.7%) than in the control, and could have significant biotechnological application due to lower cellulose loss. A key mechanism of carbohydrate component convertibility enhancement was lignin removal in the biomass. Long time consumption, the low sugar yields and unpredictable fungal response still remain the challenge of the fungal pretreatment process.

Download Full-text

Transcriptomics analysis reveals the high biodegradation efficiency of white-rot fungus Phanerochaete sordida YK-624 on native lignin

10.21203/rs.2.20480/v1 ◽

2020 ◽

Author(s):

Jianqiao Wang ◽

Tomohiro Suzuki ◽

Hideo Dohra ◽

Toshio Mori ◽

Hirokazu Kawagishi ◽

...

Keyword(s):

Gene Expression ◽

Lignin Degradation ◽

Ligninolytic Enzymes ◽

Organic Matrix ◽

Tca Cycle ◽

Underlying Mechanism ◽

White Rot ◽

White Rot Fungus ◽

Lignin Biodegradation ◽

Phanerochaete Sordida

Abstract Background Lignocellulosic biomass is an organic matrix composed of cellulose, hemicellulose, and lignin. In nature, lignin degradation by basidiomycetes is the key step in lignocellulose decay. The white-rot fungus Phanerochaete sordida YK-624 (YK-624) has been extensively studied due to its high lignin degradation ability. In our previous study, it was demonstrated that YK-624 can secrete lignin peroxidase and manganese peroxidase for lignin degradation. However, the underlying mechanism for lignin degradation by YK-624 remains unknown.Results Here, we analyzed YK-624 gene expression following growth under ligninolytic and nonligninolytic conditions and compared the differentially expressed genes in YK-624 to those in the model white-rot fungus P. chrysosporium by next-generation sequencing. More ligninolytic enzymes and lignin-degrading auxiliary enzymes were upregulated in YK-624. This might explain the high degradation efficiency of YK-624. In addition, the genes involved in energy metabolism pathways, such as the TCA cycle, oxidative phosphorylation, lipid metabolism, carbon metabolism and glycolysis, were upregulated under ligninolytic conditions in YK-624.Conclusions In the present study, the first differential gene expression analysis of YK-624 under ligninolytic and nonligninolytic conditions was reported. The results obtained in this study indicated that YK-624 produces more energy- and lignin-degrading enzymes for more efficient lignin biodegradation.

Download Full-text