Development and validation of a simple high performance thin layer chromatography method combined with direct 1,1-diphenyl-2-picrylhydrazyl assay to quantify free radical scavenging activity in wine

2016 ◽  
Vol 197 ◽  
pp. 285-290 ◽  
Author(s):  
Snezana Agatonovic-Kustrin ◽  
David W. Morton ◽  
Ahmad P. Yusof
Keyword(s):  
Free Radical ◽  
Thin Layer ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Chromatography Method ◽  
Development And Validation ◽  
Layer Chromatography

scholarly journals HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY ANALYSIS AND FREE RADICAL SCAVENGING POTENTIAL OF SOUTH INDIAN ORTHODOX BLACK TEA

2018 ◽  
Vol 11 (9) ◽  
pp. 449
Author(s):  
Mahalakshmi Rajamani ◽  
Kalaivani Krishnasamy ◽  
Nishadh Abubakker
Keyword(s):  
Free Radical ◽  
Thin Layer ◽  
Hydroxyl Radical ◽  
High Performance ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Black Tea ◽  
Free Radical Scavenging ◽  
South Indian ◽  
Layer Chromatography

Objective: The objective of this study was to evaluate the free radical scavenging potential and high-performance thin-layer chromatography (HPTLC) fingerprinting of the ethanolic extract of south Indian orthodox black tea (OBT).Methods: Phytochemical analysis was carried out using standard methods, and free radical scavenging activity of the extract was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), superoxide anion (SOD), and hydroxyl radical scavenging capacities. The ethanolic extract of OBT was loaded in the pre-coated HPTLC plates (silica gel 60 F 254) E-MERCK KGaA. HPTLC was carried out with toluene: ethyl acetate: diethylamine (7:2:1), chloroform: methanol:formic acid (8.5:1.0:0.5), and butanol: isopropyl alcohol (1:1) as mobile phase for alkaloids, flavonoids, and terpenoids, respectively.Results: HPTLC results confirmed that the extract contained several potential active components such as flavonoids, alkaloids, and terpenoids as the slides revealed multicolored bands of varying intensities. Extract of OBT reliably showed the total phenolics 132.27 mg/g, flavonoids 72.52 mg/g, and alkaloids 66.01 mg/g of dry matter. The IC50 value of OBT for DPPH was found to be 372.22 μg/ml, SOD 311.93 μg/ml, NO 362.17 μg/ml, hydroxyl radical 342.14 μg/ml, and reducing power 178.54 μg/ml.Conclusion: The HPTLC fingerprinting profile developed for ethanolic extract will help in proper identification and quantification of marker compounds. The ethanolic extract of OBT was found to possess a wide range of phytochemicals with excellent antioxidant properties. This information may help to choose the best beverage to be consumed in the future.

scholarly journals Free Radical Scavenging Activity and Antioxidants of Hydrocotyle vulgaris L. (Pennywort): Baseline Study in Developing Biocosmetic-Antidote for Pathological Aging

2018 ◽  
Vol 10 ◽  
pp. 1-12
Author(s):  
Romnick Ureta ◽  
Siegfred Mejico ◽  
Yvonne Maranan
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Pathological Aging ◽  
Vis Spectroscopy

Pathological aging due to harmful free radicals and oxidative stress has been a serious threat to human health (Kensella & He, 2008). Thus, the study aimed to evaluate the free radical scavenging activity, antioxidants, essential vitamins, and physicochemical properties of Hydrocotyle vulgaris L. (Pennywort) which could serve as basis in developing a biocosmetic antidote for pathological aging. H.vulgaris L. plant was harvested, air dried and then extracted via soxhlet. UV-vis spectroscopy was used for scavenging activity and antioxidants of the plant while High Performance Liquid Chromatography (HPLC) was applied for the identification of essential vitamins. Phytochemicals, pH and spreadability values of the plant were also tested. Results revealed that H.vulgaris L. has an abundant presence of flavonoid and alkaloid while traced presence of tannin. Good trend for scavenging activity was also observed exhibiting low absorbance of possible free radicals with IC50 of 29.75 and antioxidant activity of 158.13 (Total Phenolics as gallic acid in %w/w). Meanwhile, β-carotene (10.4 mg/kg), Riboflavin (4.08mg/kg), and Vitamins C (70.2mg/kg) and E (26.9mg/kg) were also found in the plant. Non-irritating pH levels of 5.7 and 7.14 for 50% and 75% concentrations of the extracts with good spreadability value of 17.51 g∙cm/sec (for 75% concentration) were also obtained. Based on the findings of the study, Pennywort has a very promising therapeutic characteristics with its good scavenging activity and antioxidants. Thus, the development of a biocosmetic product that could serve as an antidote for pathological aging from Pennywort appeared to be very apparent.

scholarly journals NMR characterization and free radical scavenging activity of pheophytin ‘A’ from the leaves of Dissotis rotundifolia

2021 ◽  
Vol 35 (1) ◽  
pp. 207-215
Author(s):  
C. Friday ◽  
O. UchennaIgwe ◽  
U. C. Akwada
Keyword(s):  
Free Radical ◽  
Thin Layer ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Pheophytin A ◽  
Chromatographic Techniques ◽  
Nmr Characterization

Dissotis rotundifolia is used traditionally in various parts of Africa for the treatment of various ailments. The air dried and pulverized leaves of Dissotis rotundifolia was extracted with distilled n-hexane and ethylacetate, using Soxhlet apparatus. Isolation and purification were carried out using column and thin layer chromatographic techniques. Fraction B14 gave a single spot on thin layer chromatography with a retention factor (Rf) value of 0.57. Characterization of B14 was doneusing 1H-NMR, 13C-DEPT, COSY, HSQC, HMBC and by direct comparison with literature values and pheophytin A was proposed as the structure of the compound. Pheophytin A exhibited a free radical scavenging activity of 10.10±0.05 and 19.51±0.02% at minimum and maximum concentrations of 1.00 and 4.00 mg/mL, respectively. The acclaimed ethnomedicinal uses of D. rotundifolia by the African natives could be linked to the presence of pheophytin A and other phytoconstituents in the plant. This is the first report of the isolation of pheophytin A from D. rotundifolia.                     KEY WORDS: Dissotis rotundifolia, Pheophytin A, Chromatographic techniques, Free radicals, NMR   Bull. Chem. Soc. Ethiop. 2021, 35(1), 207-215. DOI: https://dx.doi.org/10.4314/bcse.v35i1.18      

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