Investigation on antioxidant, angiotensin converting enzyme and dipeptidyl peptidase IV inhibitory activity of Bambara bean protein hydrolysates

2018 ◽  
Vol 250 ◽  
pp. 162-169 ◽  
Author(s):  
Martin Alain Mune Mune ◽  
Samuel René Minka ◽  
Thomas Henle
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Inhibitory Activity ◽  
Dipeptidyl Peptidase ◽  
Protein Hydrolysates ◽  
Dipeptidyl Peptidase Iv ◽  
Converting Enzyme ◽  
Bean Protein

Dietary regulation of rat intestinal angiotensin-converting enzyme and dipeptidyl peptidase IV

1993 ◽  
Vol 264 (6) ◽  
pp. G1153-G1159 ◽  
Author(s):  
Y. Suzuki ◽  
R. H. Erickson ◽  
A. Sedlmayer ◽  
S. K. Chang ◽  
Y. Ikehara ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Fold Increase ◽  
Immunoblot Analysis ◽  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Converting Enzyme ◽  
Brush Border Membranes ◽  
Dpp Iv

The small intestinal brush-border membrane contains several peptidases that are involved in the hydrolysis of dietary peptides containing proline. A high-proline (gelatin) diet was administered to one of several groups of rats to study its possible regulatory effect on levels of two prolyl peptidases, namely angiotensin-converting enzyme (ACE) and dipeptidyl peptidase IV (DPP IV). Groups of rats were maintained on isocaloric diets containing either low (4%), normal (17%), or high (50%) protein (casein) or high (50%) gelatin. After 7 days, brush-border membranes and total RNA were prepared from the small intestine. ACE activity was 3- to 10-fold higher in brush-border membranes from the gelatin group compared with the low-protein group. DPP IV exhibited a three- to sixfold increase. Immunoblot analysis of brush-border membrane-associated ACE protein indicated a six- to eightfold increase in the high-gelatin group. There was also a 1.5- to 3-fold increase in steady-state levels of ACE and DPP IV mRNA. These results suggest that a diet high in proline (gelatin) is particularly effective in increasing intestinal levels of these two enzymes.

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