Sensitive identification of milk protein allergens using on-line combination of transient isotachophoresis/micellar electrokinetic chromatography and capillary isoelectric focusing in fused silica capillary with roughened part

2022 ◽  
pp. 131986
Author(s):  
Marie Horká ◽  
Jiří Šalplachta ◽  
Pavel Karásek ◽  
Michal Roth
Keyword(s):  
Isoelectric Focusing ◽  
Milk Protein ◽  
Micellar Electrokinetic Chromatography ◽  
Fused Silica ◽  
Electrokinetic Chromatography ◽  
Capillary Isoelectric Focusing ◽  
Fused Silica Capillary ◽  
On Line ◽  
Silica Capillary

scholarly journals Clinical Application of Capillary Isoelectric Focusing on Fused Silica Capillary for Determination of Hemoglobin Variants

1997 ◽  
Vol 43 (9) ◽  
pp. 1798-1799 ◽  
Author(s):  
Amin A Mohammad ◽  
Anthony O Okorodudu ◽  
Michael G Bissell ◽  
Pat Dow ◽  
Gregg Reger ◽  
...  
Keyword(s):  
Clinical Application ◽  
Isoelectric Focusing ◽  
Fused Silica ◽  
Capillary Isoelectric Focusing ◽  
Fused Silica Capillary ◽  
Hemoglobin Variants ◽  
Silica Capillary

Isoelectric Focusing in Continuously Tapered Fused Silica Capillary Prepared by Etching with Supercritical Water

2013 ◽  
Vol 85 (9) ◽  
pp. 4296-4300 ◽  
Author(s):  
Karel Šlais ◽  
Marie Horká ◽  
Pavel Karásek ◽  
Josef Planeta ◽  
Michal Roth
Keyword(s):  
Isoelectric Focusing ◽  
Supercritical Water ◽  
Fused Silica ◽  
Fused Silica Capillary ◽  
Silica Capillary

scholarly journals Determination of N-methyl-2-pyrrolidone and its metabolites in urine by micellar electrokinetic chromatography

2011 ◽  
Vol 9 (5) ◽  
pp. 825-833 ◽  
Author(s):  
Jana Lokajová ◽  
Simo Porras ◽  
Eivor Elovaara ◽  
Susanne Wiedmer
Keyword(s):  
Solid Phase ◽  
Background Electrolyte ◽  
Sodium Dodecyl ◽  
Fused Silica ◽  
Rat Urine ◽  
Fused Silica Capillary ◽  
On Line ◽  
Electrokinetic Capillary Chromatography ◽  
Silica Capillary

AbstractA fast and accurate micellar electrokinetic capillary chromatography (MEKC) method was developed for monitoring N-methyl-2-pyrrolidone (NMP) exposure. Baseline separation of NMP and its main metabolites: 5-hydroxy-N-methyl-2-pyrrolidone (5HNMP), N-methylsuccinimide (MSI), 2-hydroxy-N-methylsuccinimide (2HMSI), and 2-pyrrolidone (2P) was obtained within 6 min in an uncoated fused silica capillary using 5 mM phosphate buffer and 140 mM sodium dodecyl sulfate (pH 7.1) as background electrolyte (BGE). On-line UV-detection was performed at 200 nm and the applied electric field was 400 V cm−1. Possible interference of BGE-induced system peaks on separation was investigated by computer simulation and no such interference was observed. The developed MEKC method combined with solid phase extraction for sample preparation was successfully applied to the analysis of urine of rats exposed to NMP. The urinary excretion was determined in 0–6 h and 6–24 h specimens collected after an intragastic administration of 308 mg NMP / kg rat body weight. The results of NMP disposition kinetics in rat urine are reported for NMP and metabolites.

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