Exploring the effects of pectate and pectate lyase on the fruit softening and transcription profiling of Solanum lycopersicum

Food Control ◽  
2021 ◽  
pp. 108636
Author(s):  
Lieqin Shi ◽  
Qin Liu ◽  
Qinghua Qiao ◽  
Yulin Zhu ◽  
Wei Huang ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Pectate Lyase ◽  
Fruit Softening ◽  
Transcription Profiling

scholarly journals Re-evaluation of the nor mutation and the role of the NAC-NOR transcription factor in tomato fruit ripening

2020 ◽  
Vol 71 (12) ◽  
pp. 3560-3574 ◽  
Author(s):  
Ying Gao ◽  
Wei Wei ◽  
Zhongqi Fan ◽  
Xiaodan Zhao ◽  
Yiping Zhang ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Tomato Fruit ◽  
Pectate Lyase ◽  
Ethylene Biosynthesis ◽  
Fruit Softening ◽  
Wild Type ◽  
Gain Of Function ◽  
Carotenoid Accumulation ◽  
Acid Protein ◽  
Tomato Fruit Ripening

Abstract The tomato non-ripening (nor) mutant generates a truncated 186-amino-acid protein (NOR186) and has been demonstrated previously to be a gain-of-function mutant. Here, we provide more evidence to support this view and answer the open question of whether the NAC-NOR gene is important in fruit ripening. Overexpression of NAC-NOR in the nor mutant did not restore the full ripening phenotype. Further analysis showed that the truncated NOR186 protein is located in the nucleus and binds to but does not activate the promoters of 1-aminocyclopropane-1-carboxylic acid synthase2 (SlACS2), geranylgeranyl diphosphate synthase2 (SlGgpps2), and pectate lyase (SlPL), which are involved in ethylene biosynthesis, carotenoid accumulation, and fruit softening, respectively. The activation of the promoters by the wild-type NOR protein can be inhibited by the mutant NOR186 protein. On the other hand, ethylene synthesis, carotenoid accumulation, and fruit softening were significantly inhibited in CR-NOR (CRISPR/Cas9-edited NAC-NOR) fruit compared with the wild-type, but much less severely affected than in the nor mutant, while they were accelerated in OE-NOR (overexpressed NAC-NOR) fruit. These data further indicated that nor is a gain-of-function mutation and NAC-NOR plays a significant role in ripening of wild-type fruit.

scholarly journals Manipulation of Strawberry Fruit Softening by Antisense Expression of a Pectate Lyase Gene

2002 ◽  
Vol 128 (2) ◽  
pp. 751-759 ◽  
Author(s):  
Silvia Jiménez-Bermúdez ◽  
José Redondo-Nevado ◽  
Juan Muñoz-Blanco ◽  
José L. Caballero ◽  
José M. López-Aranda ◽  
...  
Keyword(s):  
Pectate Lyase ◽  
Fruit Softening ◽  
Strawberry Fruit ◽  
Antisense Expression

scholarly journals Antisense inhibition of a pectate lyase gene supports a role for pectin depolymerization in strawberry fruit softening

2008 ◽  
Vol 59 (10) ◽  
pp. 2769-2779 ◽  
Author(s):  
N. Santiago-Domenech ◽  
S. Jimenez-Bemudez ◽  
A. J. Matas ◽  
J. K. C. Rose ◽  
J. Munoz-Blanco ◽  
...  
Keyword(s):  
Pectate Lyase ◽  
Fruit Softening ◽  
Antisense Inhibition ◽  
Strawberry Fruit

Effect of simultaneous down-regulation of pectate lyase and endo-β-1,4-glucanase genes on strawberry fruit softening

2012 ◽  
Vol 31 (2) ◽  
pp. 313-322 ◽  
Author(s):  
Sabry M. Youssef ◽  
Iraida Amaya ◽  
José M. López-Aranda ◽  
Rafael Sesmero ◽  
Victoriano Valpuesta ◽  
...  
Keyword(s):  
Pectate Lyase ◽  
Fruit Softening ◽  
Strawberry Fruit ◽  
Down Regulation

Evaluación de tres enraizadores comerciales en la producción de plántulas de tomate indeterminado (Solanum lycopersicum (L.) Lam)

2019 ◽  
Vol 12 (12) ◽  
Author(s):  
M. Arébalo-Madrigal ◽  
J.L. Escalante-González ◽  
J.B. Yáñez-Coutiño ◽  
M.E. Gallegos-Castro
Keyword(s):  
Solanum Lycopersicum ◽  
Solanum Lycopersicum L

Objetivo: Evaluar el desarrollo de plántula de tomate indeterminado bajo condiciones protegidas, aplicando  tres enraizadores  y un testigo para aumentar la producción del cultivo en la región. Diseño/metodología/aproximación: se utilizó bajo un diseño experimental en bloques completamente al azar, el cual consistió de cuatro tratamientos correspondiendo a cada uno de los bloques, donde cada bloque pertenecía a cuatro charolas de unicel de 200 cavidades con sustrato de BM2, con cuatro repeticiones cada uno, teniendo 15 unidades experimentales por tratamiento, sumando un total de 60 unidades experimentales, teniendo un total de 240 plántulas de tomate por todo el experimento. Resultados: Como resultado se obtuvo que el enraizador de Phyto Root  tuvo un gran efecto en cuanto al desarrollo de altura, grosor de tallo, numero de hojas, biomasa aérea y peso del cepellón, parámetros importantes que debe tener una plántula para su desarrollo y crecimiento al momento de trasplante a campo. Limitaciones del estudio/implicaciones: El manejo agronómico desde la siembra en charolas, es necesario que sea uniforme en todos los tratamientos y las repeticiones para tener mejores resultados en cuanto el efecto de los enraizadores. Hallazgos/conclusiones: Para obtener plántulas de buena calidad en el momento de trasplante a campo se le recomienda a la empresa y a los productores de plántulas utilizar el tratamiento de Phyto Root, ya que fue la que mejor respuesta tuvo.

Effects of Defoliation and Foliar Application of Gibberellic Acid on Fruit Softening after Alcohol Treatment of Japanese Persimmon 'Hiratanenashi'.

1994 ◽  
Vol 62 (4) ◽  
pp. 867-875 ◽  
Author(s):  
Hiroyuki Itamura ◽  
Tadaaki Fukushima ◽  
Toshio Kitamura ◽  
Hisashi Harada ◽  
Satoshi Taira ◽  
...  
Keyword(s):  
Gibberellic Acid ◽  
Foliar Application ◽  
Alcohol Treatment ◽  
Fruit Softening ◽  
Japanese Persimmon

Expression of antioxidant genes can increase cold resistance in tomato (Solanum lycopersicum L.)

2020 ◽  
Vol 53 (2) ◽  
Author(s):  
Javaria Chand ◽  
Rafiq Ahmad ◽  
Muhammad Shahzad ◽  
Muhammad Sohail Khan ◽  
Noorullah Khan ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Cold Resistance ◽  
Antioxidant Genes ◽  
Solanum Lycopersicum L
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