Saccharum Granorum ameliorated mitochondrial dysfunction in chronic atrophic gastritis rats using organelle-specific metabolomics and network pharmacology approaches

2020 ◽  
Vol 136 ◽  
pp. 109503
Author(s):  
Yuetao Liu ◽  
Xinqi Li ◽  
Xuemei Qin
Keyword(s):  
Mitochondrial Dysfunction ◽  
Atrophic Gastritis ◽  
Chronic Atrophic Gastritis ◽  
Network Pharmacology

Network pharmacology to unveil the mechanism of Moluodan in the treatment of chronic atrophic gastritis

Phytomedicine ◽  
2021 ◽  
pp. 153837
Author(s):  
Wuai Zhou ◽  
Huan Zhang ◽  
Xin Wang ◽  
Jun Kang ◽  
Wuyan Guo ◽  
...  
Keyword(s):  
Atrophic Gastritis ◽  
Chronic Atrophic Gastritis ◽  
Network Pharmacology

scholarly journals Screening and Identification of Molecular Targets Involved in Preventing Gastric Precancerous Lesions in Chronic Atrophic Gastritis by Qilianshupi Decoction

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Yameng Zhang ◽  
Chao Li ◽  
Shanmei Sun ◽  
Zhiqun Cao ◽  
Jian Chen ◽  
...  
Keyword(s):  
Atrophic Gastritis ◽  
Telomere Length ◽  
Precancerous Lesions ◽  
Animal Experiments ◽  
Molecular Targets ◽  
Chronic Atrophic Gastritis ◽  
Telomerase Activity ◽  
Network Pharmacology ◽  
Screening And Identification ◽  
Tract Disease

Chronic atrophic gastritis (CAG) is a common and possibly precancerous digestive tract disease. Development of drugs with effect of preventing precancerous lesions draws the eyes of global researchers. Qilianshupi decoction (QLSP) is a Traditional Chinese Medicine (TCM) that is commonly used to treat CAG, but few studies have explored the mechanism of QLSP on treating CAG. This study investigated the molecular targets of the component herbs of QLSP in preventing precancerous lesions based on network pharmacology. Network pharmacology analysis revealed that the 6 herbs regulated multiple CAG-related genes, among which the most important were cancer-related pathway (apoptosis, p53, and VEGF) and epithelial cell signaling in Helicobacter pylori infection. Further animal experiments showed that the expression of survivin and p53 in precancerous lesions of CAG rats was significantly increased which was suppressed by QLSP. Moreover, telomerase activity was inhibited in precancerous lesions of CAG rats, and telomere length of gastric mucosa was increased, which was reversed by QLSP. Our results suggest that the components of QLSP prevents gastric precancerous lesions through decreasing the expression of survivin and p53 and regulating telomerase activity and telomere length in CAG.

scholarly journals Exploring the potential pharmacodynamic material basis and pharmacologic mechanism of the Fufang-Xialian-Capsule in chronic atrophic gastritis by network pharmacology approach based on the components absorbed into the blood

2018 ◽  
Vol 5 (6) ◽  
pp. 171806
Author(s):  
Shizhe Li ◽  
Tengfei Xu ◽  
Shu Liu ◽  
Zhiqiang Liu ◽  
Zifeng Pi ◽  
...  
Keyword(s):  
Atrophic Gastritis ◽  
Drug Targets ◽  
High Performance ◽  
Chronic Atrophic Gastritis ◽  
Rat Plasma ◽  
Network Pharmacology ◽  
Absorption Analysis ◽  
Network Approach ◽  
Flight Mass Spectrometry ◽  
Material Basis

In this study, a new network pharmacology approach based on the components absorbed into the blood was used to investigate the pharmacodynamic material basis and the pharmacologic mechanism of the Fufang-Xialian-Capsule ( FXL ) in treating chronic atrophic gastritis (CAG). Initially, we confirmed the components absorbed into the blood by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. Then, the network approach, which was based on the results of components absorbed into the blood, was used to analyse the pharmacodynamic material basis and the pharmacologic mechanism of FXL on treating CAG. As a result, 22 absorbed components were found in rat plasma. Given the results of the absorption analysis of the components, eight pathways associated with CAG development were found. The targets linked to these pathways are the drug targets of FXL in CAG treatment. The components associated with these targets are the potential pharmacodynamic material basis and exert synergy in regulating pathways during CAG treatment.

scholarly journals A metabolomics-binding network pharmacology study of Zuojin Pill intervention in chronic atrophic gastritis induced by Helicobacter pylori

2020 ◽  
Author(s):  
Shihua Wu ◽  
Xing Chen ◽  
Honghong Liu ◽  
Ruilin Wang ◽  
Jianyu Li ◽  
...  
Keyword(s):  
Succinic Acid ◽  
Atrophic Gastritis ◽  
Tca Cycle ◽  
Serum Levels ◽  
Gastrointestinal Diseases ◽  
Chronic Atrophic Gastritis ◽  
Network Pharmacology ◽  
Pcr Analysis ◽  
Therapeutic Effects ◽  
Aconitic Acid

Abstract Background Zuojin Pill (ZJP) is widely used for the treatment of gastrointestinal diseases, while its specific mechanism has not been systematically investigated. The aim of this study was to explore the mechanism of intervention of ZJP in chronic atrophic gastritis (CAG) through metabolomics combined with network pharmacology.Materials and methods Potential metabolites and possible pathways for ZJP treatment of CAG were explored using a UPLC-Q-TOF/MS-based metabolomics technique. The key targeting mechanism of ZJP for CAG was explored by combining the analysis with network pharmacology.Results ZJP significantly reduced serum levels of IL-1β, IL-6, IL-10 and iNOS, and improved pathological characteristics. Metabolomic results indicated that the therapeutic effect of ZJP was mainly related to ten metabolites, including choline, L-threonine, hydroxypyruvic acid, creatine, taurine, succinic acid, cis-aconitic acid, citric acid, succinic acid semialdehyde and uric acid. Pathway analysis showed that the treatment of CAG by ZJP was associated with taurine and hypotaurine metabolism, glyoxylate and dicarboxylate metabolism, glycine, serine and threonine metabolism, glycerophospholipid metabolism, citrate cycle (TCA cycle), alanine, aspartate and glutamate metabolism, butanoate metabolism and purine metabolism. Validation of potential metabolic markers and key targets of network pharmacology by RT-PCR analysis showed that ZJP significantly down-regulated a series of inflammatory markers, such as MAPK1, PKIA, RB1, SCN5A, RXRA, E2F1, PTGS1, IGF2, ADRB1, ADRA1B, PTGS2, and GABRA1.Conclusion For the first time, a combination of metabolomics and network pharmacology has been used to clarify the therapeutic effects of ZJP on CAG and its relationship to the regulation of multiple metabolic pathways.

scholarly journals Study of Zuojin Pill in Treating Chronic Atrophic Gastritis by UPLC-Q-TOF/MS Based on Serum and Urine Metabolomics Combined with Network Pharmacology

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Shihua Wu ◽  
Xing Chen ◽  
Honghong Liu ◽  
Ruilin Wang ◽  
Jianyu Li ◽  
...  
Keyword(s):  
Succinic Acid ◽  
Atrophic Gastritis ◽  
Tca Cycle ◽  
Gastrointestinal Diseases ◽  
Chronic Atrophic Gastritis ◽  
Network Pharmacology ◽  
Pcr Analysis ◽  
Therapeutic Effects ◽  
Aconitic Acid ◽  
Tof Ms

Zuojin Pill (ZJP) is widely used for the treatment of gastrointestinal diseases, while its specific mechanism has not been systematically investigated. The aim of this study was to explore the therapeutic effects and potential mechanism of ZJP in chronic atrophic gastritis (CAG) through UPLC-Q-TOF/MS-based metabolomics combined with network pharmacology. ZJP and omeprazole significantly reduce contents of IL-1β, IL-6, IL-10, and iNOS and improve pathological characteristics. Metabolomic results indicated that the therapeutic effects of ZJP were mainly related to ten metabolites, namely, choline, L-threonine, hydroxypyruvic acid, creatine, taurine, succinic acid, cis-aconitic acid, citric acid, succinic acid semialdehyde, and uric acid. Pathway analysis showed that the treatment of CAG by ZJP was associated with taurine and hypotaurine metabolism; glyoxylate and dicarboxylate metabolism; glycine, serine, and threonine metabolism; glycerophospholipid metabolism; citrate cycle (TCA cycle), alanine, aspartate, and glutamate metabolism; butanoate metabolism; and purine metabolism. Validation of metabolic markers and key targets of network pharmacology through RT-PCR analysis showed that ZJP significantly downregulated a series of inflammatory markers, such as MAPK1, PKIA, RB1, SCN5A, RXRA, E2F1, PTGS1, IGF2, ADRB1, ADRA1B, PTGS2, and GABRA1. This study was the first to use a combination of metabolomics and network pharmacology to clarify the therapeutic effects of ZJP on CAG and the regulation of multiple metabolic pathways.

scholarly journals Integrating Network Pharmacology and Experimental Validation to Investigate the Mechanisms of Huazhuojiedu Decoction to Treat Chronic Atrophic Gastritis

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Xinyu Hao ◽  
Yu Liu ◽  
Pingping Zhou ◽  
Qian Jiang ◽  
Zeqi Yang ◽  
...  
Keyword(s):  
Atrophic Gastritis ◽  
Experimental Validation ◽  
Precancerous Lesions ◽  
Animal Experiments ◽  
Interaction Network ◽  
Chronic Atrophic Gastritis ◽  
Network Pharmacology ◽  
Pathway Enrichment Analysis ◽  
Active Components ◽  
Egfr Expression

Background. Chronic atrophic gastritis (CAG) is an important stage in the normal gastric mucosa’s transformation into gastric cancer. Huazhuojiedu decoction (HZJD), a Chinese herbal preparation, has proven clinically effective to treat CAG. However, few studies have explored the mechanism of HZJD in CAG treatment. Purpose. This study aimed to shed light on the mechanisms underlying HZJD decoction CAG treatment using a network pharmacology approach and experimental validation. Methods. The active components of HZJD decoction were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform. Their targets were predicted through the SwissTargetPrediction database. Disease targets were screened using the GeneCards database. The disease and drug prediction targets were intersected to select the common potential therapeutic targets, which then were input into the Search Tool for the Retrieval of Interacting Genes to build a protein-protein interaction network. The “herb-compound-target-disease” and the “herb-target-pathway” network diagrams were constructed in Cytoscape 3.3.0. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of effective targets were performed using the Database for Annotation, Visualization, and Integrated Discovery. Finally, the core targets were preliminarily verified by CAG rat model. The gastric mucosa’s histopathological changes were observed via hematoxylin-eosin staining. The expressions of MAPK1, AKT1, TNF, VEGFA, and EGFR were detected by western blot and quantitative real-time reverse transcription-polymerase chain reaction. Results. A total of 155 nodes, including 20 putative targets of HZJD decoction, were selected as core hubs based on topological importance and were closely associated with the regulation of cell proliferation, apoptotic process, and cancer-related pathways (AKT1, TNF, VEGFA, and EGFR) in CAG. Further animal experiments showed that the expression of AKT1 in CAG rats was significantly increased, which was suppressed by HZJD decoction. TNF and VEGFA expression increased in the model group, but did not change in the HZJD group. MAPK1 and EGFR expression showed no significant differences among control, model, and HZJD groups. Conclusion. Taken together, the results suggest that the components of HZJD decoction can alleviate and prevent the severity of gastric precancerous lesions via AKT1 inhibition in CAG.

Chronic Atrophic Gastritis: A Review

2018 ◽  
Vol 37 (3) ◽  
pp. 241-259 ◽  
Author(s):  
Yifei Li ◽  
Ran Xia ◽  
Bo Zhang ◽  
Chunsheng Li
Keyword(s):  
Atrophic Gastritis ◽  
Chronic Atrophic Gastritis
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