Major royal jelly proteins accelerate onset of puberty and promote ovarian follicular development in immature female mice

Abstract Objectives Estrogen plays an important role in for growth and development of reproductive system in female. The major royal jelly (RJ) has a significant estrogenic-like effect for many female animals. The major RJ proteins (MRJPs) are the central constituents responsible for the physiological activities of RJ. In recent years, we have found that MRJPs possesses functions to increase fecundity associated with estrogenic effect in Drosophila. However, if MRJPs are the specific active ingredient mediating estrogenic effect of RJ and related action mechanism in mammalians remains unclear. Methods Neonatal immature female mice (FC) were divided into four groups fed MRJPs with doses of 0, 125, 250 and 500 mg/kg/body weight (M125, M250 and M500), respectively. The freeze-dried MRJPs powder was administrated daily for 45 consecutive days. The effects of MRJPs on the puberty onset, ovarian follicular development and serum estradiol levels in FC were evaluated. Results The times of estrus in M125, M250 and M500 were accelerated by 10.7%, 15.5% and 10.7%, the ovarian index of M125 and M250 and M500 were increased by 26.8% and 32.1% and 1.7%. The number of secondary follicles in M125 and M250 and M500 were increased by 50.7%,78.8% and 38.6%, the Graafian follicles in M125 and M250 and M500 were increased by 600.0% and 774.0% and 150.0%. M500 induced multi-oocyte follicles. The serum estradiol levels of M125, M250 and M500 were increased by 47.1%, 64.9% and 31.1%, the action of MRJPs raising hormone secretion level is mainly via upregulating expression of ERβ gene. Antioxidant parameters of ovarian tissue showed that the malondialdehyde levels in M125 and M250 were decreased, the superoxide dismutase activities and glutathione peroxidase activities in M125 and M250 were increased, respectively. Conclusions Oral administration of MRJPs may accelerate onset of puberty and promote follicular development in immature FM. The reproductive performance promotion of MRJPs was associated with raising estrogenic activity and antioxidant potential to reproductive system, upregulating expression of ERβ gene to raise hormone secretion and promote ovary development in FM. Funding Sources The National Natural Science Foundation of China and US Department of Agriculture, Agriculture Research Service.

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Gene injections of vascular endothelial growth factor and growth differentiation factor-9 stimulate ovarian follicular development in immature female rats

Fertility and Sterility ◽

10.1016/j.fertnstert.2007.06.043 ◽

2008 ◽

Vol 89 (5) ◽

pp. 1563-1570 ◽

Cited By ~ 10

Author(s):

Takashi Shimizu ◽

Koji Iijima ◽

Yoshinori Ogawa ◽

Hitoshi Miyazaki ◽

Hiroshi Sasada ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Follicular Development ◽

Female Rats ◽

Vascular Endothelial ◽

Immature Female ◽

Differentiation Factor ◽

Growth Differentiation Factor 9 ◽

Ovarian Follicular Development ◽

Endothelial Growth Factor

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119. THE EFFECT OF BMPR-IB IMMUNIZATION IN IMMATURE FEMALE MICE

Reproduction Fertility and Development ◽

10.1071/srb09abs119 ◽

2009 ◽

Vol 21 (9) ◽

pp. 38

Author(s):

I. M. Ciller ◽

J. D. McFarlane ◽

J. R. McFarlane

Keyword(s):

Reproductive System ◽

Follicular Development ◽

Significant Degree ◽

Type I ◽

Female Reproductive System ◽

Immature Female ◽

Ovarian Weight ◽

Female Mice ◽

Signaling Components

The female reproductive system is regulated by well known endocrine signaling components and the less well understood autocrine and paracrine signaling components by growth factors and their receptors. The type I Bone Morphogenetic Protein Receptor IB (BMPR-IB) is a serine/threonine kinase that facilitates the signaling of various TGF-β family members. BMPR-IB is believed to have a substantial role in reproduction as demonstrated by the hyperprolific effect on ovulation in Booroola ewes which harbor a natural BMPR-IB receptor mutation, as well as the sterility induced by the BMPR-IB knockout in female mice. Maturation of the ovary and enhancement of follicular growth are known to be dependent on gonadotrophin stimulation from the pituitary. This study aims to elucidate the role of BMPR-IB in the female reproductive system by passive immune neutralization. Immature female mice (21 days old) were passively immunized against BMPR-IB via subcutaneous injections of 100 μl PBS containing anti BMPR-IB Ig in the absence and presence of equine chorionic gonadotrophin (eCG). The preparations where administered on day one and three of a four day experiment. On day four mice were asphyxiated with CO2 and the ovaries were removed and weighed. In immature mice immunization against BMPR-IB ovarian weights were not different from control, while eCG significantly increased ovarian weight compared to the controls. In combination immunization against BMPR-IB further augmented the gonadotrophin-stimulated increase in ovarian weight to a significant degree. The suppressive effect of BMPR-IB signaling on follicular development in response to gonadotrophin stimulation indicates a possible developmental role of this receptor in the initiation of follicle growth in response to increased circulatory gonadotrophins and prevention of precocious puberty.

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HUMAN URINARY GONADOTROPHINS ASSAYED BY DIFFERENT RESPONSES IN THE SAME ANIMAL

Acta Endocrinologica ◽

10.1530/acta.0.xxxiv0375 ◽

1960 ◽

Vol XXXIV (III) ◽

pp. 375-389 ◽

Cited By ~ 1

Author(s):

P. S. Brown

Keyword(s):

Postmenopausal Women ◽

Ovarian Response ◽

The Other ◽

Relative Potency ◽

Vaginal Opening ◽

Serial Sections ◽

Immature Female ◽

Female Mice ◽

Marked Disparity ◽

Relative Potencies

ABSTRACT Selected human urinary gonadotrophins were assayed against one another using various measures of response in the same immature female mice. Intact or hypophysectomized animals were used and in some experiments the results of hypophysectomy were checked in complete serial sections. Extracts from the urine of two subjects with Turner's syndrome were compared. In intact mice, the relative potency judged by the ovarian response differed from that shown by the uterine response and the 95 % fiducial limits of the two estimates did not overlap. When the mice were hypophysectomized, one extract became much less potent while the other did not. Similar differences were shown in the response of intact mice to urinary extracts from two subjects with Klinefelter's syndrome. There was a marked disparity between the relative potencies shown by the uterine response and by the incidence of vaginal opening. Similar differences were not shown between the responses to different extracts from the urine of normal postmenopausal women, but these extracts were known to differ little in quality. The results are interpreted in terms of qualitative differences between human urinary gonadotrophins.

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