HUMAN URINARY GONADOTROPHINS ASSAYED BY DIFFERENT RESPONSES IN THE SAME ANIMAL

1960 ◽  
Vol XXXIV (III) ◽  
pp. 375-389 ◽  
Author(s):  
P. S. Brown
Keyword(s):  
Postmenopausal Women ◽  
Ovarian Response ◽  
The Other ◽  
Relative Potency ◽  
Vaginal Opening ◽  
Serial Sections ◽  
Immature Female ◽  
Female Mice ◽  
Marked Disparity ◽  
Relative Potencies

ABSTRACT Selected human urinary gonadotrophins were assayed against one another using various measures of response in the same immature female mice. Intact or hypophysectomized animals were used and in some experiments the results of hypophysectomy were checked in complete serial sections. Extracts from the urine of two subjects with Turner's syndrome were compared. In intact mice, the relative potency judged by the ovarian response differed from that shown by the uterine response and the 95 % fiducial limits of the two estimates did not overlap. When the mice were hypophysectomized, one extract became much less potent while the other did not. Similar differences were shown in the response of intact mice to urinary extracts from two subjects with Klinefelter's syndrome. There was a marked disparity between the relative potencies shown by the uterine response and by the incidence of vaginal opening. Similar differences were not shown between the responses to different extracts from the urine of normal postmenopausal women, but these extracts were known to differ little in quality. The results are interpreted in terms of qualitative differences between human urinary gonadotrophins.

THE EFFECT OF CERTAIN PLANT PREPARATIONS ON THE FERTILITY OF LABORATORY MAMMALS

1955 ◽  
Vol 12 (4) ◽  
pp. 273-276 ◽  
Author(s):  
JUNE EAST
Keyword(s):  
Guinea Pigs ◽  
Male Fertility ◽  
Plant Material ◽  
Vaginal Opening ◽  
Immature Female ◽  
Female Mice ◽  
Sanguisorba Officinalis

SUMMARY Mice and guinea-pigs were fed diets containing 20 and 30% whole Sanguisorba officinalis, dried and ground. In immature female mice, premature vaginal opening and constant dioestrus were produced. Adult animals showed oestrous cycles of abnormal length, owing to a prolongation of the dioestrous phase, but mated and became pregnant readily during treatment. Lactation appeared to be affected adversely. Male fertility was not impaired. The plant material showed no oestrogenic activity when fed to spayed mice, and did not produce vaginal opening in spayed guinea-pigs.

THE EFFECT OF CERTAIN PLANT PREPARATIONS ON THE FERTILITY OF LABORATORY MAMMALS

1955 ◽  
Vol 12 (4) ◽  
pp. 261-266 ◽  
Author(s):  
JUNE EAST
Keyword(s):  
Adult Female ◽  
Plant Material ◽  
Normal Diet ◽  
Vaginal Opening ◽  
Male Mice ◽  
Psoralea Corylifolia ◽  
Active Constituent ◽  
Immature Female ◽  
Female Mice ◽  
Adult Females

SUMMARY Dry powdered seeds of the legume Psoralea corylifolia L. proved to be oestrogenic when included in the normal diet of adult female mice at levels calculated to give an intake of 0·35 g or 0·175 g seed/day. Both spayed and intact females exhibited intense vaginal cornification during treatment, but the effect was reversible. The fertility of adult females was impaired by consumption of 0·35 g Psoralea seed for 37–77 days, and an abnormal proportion of matings made with treated or normal partners was infertile, resulting in pseudopregnancies. The animals recovered within 1 week of transfer to a normal diet. The fertility of male mice was not impaired by treatment for 46 days. Consumption of 0·25 g Psoralea seed daily precipitated vaginal opening in immature female mice. The oestrogenic potency of the plant material was low and psoralen(e) (furo-2:3:7:6-coumarin), known to occur in the kernel of Psoralea corylifolia, was not the active constituent.

THE EFFECTS OF VARIOUS GOITROGENS ON THE DETERMINATION OF THE RELATIVE POTENCY OF THYROID BY THE GOITRE PREVENTION ASSAY

1964 ◽  
Vol 45 (3) ◽  
pp. 370-380 ◽  
Author(s):  
G. Stuart Wiberg ◽  
John R. Carter ◽  
Norman R. Stephenson
Keyword(s):  
Thyroid Hormones ◽  
Biological Activities ◽  
Iodine Content ◽  
The Other ◽  
Relative Potency ◽  
Total Iodine ◽  
Relative Potencies ◽  
Peripheral Metabolism ◽  
House Standard

ABSTRACT It is well known that thiouracil and its derivatives not only block the synthesis of thyroid hormones but also alter the peripheral metabolism of thyroxine and triiodothyronine. Other goitrogens such as thiourea, thiocyanate and perchlorate do not have this extra-thyroidal effect. Hence the measurement of endogenous thyroid function namely »thyroxine secretion rate« as determined by the goitre-prevention response is affected by thiouracil but not presumably by the other goitrogens. The purpose of the present study was to see what effect different goitrogens had on the estimation of the relative potency of thyroid powder by the goitre prevention assay. The biological activities of pork and beef thyroid samples relative to a house standard were determined in a series of bioassays which used various goitrogens. Assays employing either thiouracil, thiourea, aminotriazole or perchlorate led to statistically equivalent potency estimates and indicated that the pork thyroid was significantly more active than the beef thyroid on the basis of the total iodine content. However, the slope of the log dose-response lines were significantly affected by the different goitrogens. When the relative potencies of pork and beef thyroid were computed on the basis of the triiodothyronine content, the three preparations had equivalent activity for each of the goitrogens. Thiocyanate proved to be toxic at the dose level employed and a valid assay was not possible.

Plasma Levels of Protein S, Protein C, and Factor X: Effects of Sex, Hormonal State and Age

1995 ◽  
Vol 74 (05) ◽  
pp. 1271-1275 ◽  
Author(s):  
C M A Henkens ◽  
V J J Bom ◽  
W van der Schaaf ◽  
P M Pelsma ◽  
C Th Smit Sibinga ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Postmenopausal Women ◽  
Protein C ◽  
Blood Donors ◽  
Premenopausal Women ◽  
Protein S ◽  
The Other ◽  
Factor X ◽  
Normal Ranges

SummaryWe measured total and free protein S (PS), protein C (PC) and factor X (FX) in 393 healthy blood donors to assess differences in relation to sex, hormonal state and age. All measured proteins were lower in women as compared to men, as were levels in premenopausal women as compared to postmenopausal women. Multiple regression analysis showed that both age and subgroup (men, pre- and postmenopausal women) were of significance for the levels of total and free PS and PC, the subgroup effect being caused by the differences between the premenopausal women and the other groups. This indicates a role of sex-hormones, most likely estrogens, in the regulation of levels of pro- and anticoagulant factors under physiologic conditions. These differences should be taken into account in daily clinical practice and may necessitate different normal ranges for men, pre- and postmenopausal women.

Effects of the aqueous extract of Dicliptera verticillata on fertility and different stages of gestation in female rats

Zygote ◽  
2021 ◽  
pp. 1-7
Author(s):  
Stéphanie Ouabo Meguem ◽  
Landry Lienou Lienou ◽  
Marie Stéphanie Chekem Goka ◽  
Richard Simo Tagne ◽  
Didiane Mefokou Yemele ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Aqueous Extract ◽  
Implantation Rate ◽  
The Other ◽  
Female Rats ◽  
High Dose ◽  
Immature Female ◽  
Implantation Sites ◽  
Parameters Analysis ◽  
Hepatic Proteins

Summary Dicliptera verticillata is a medicinal plant traditionally used in western Cameroon to cure female infertility. This experiment was designed to assess the effects of the aqueous extract of Dicliptera verticillata (AEDv) on fertility and gestation in female rats. Oral increasing doses of AEDv were administered to immature female rats over 20 d. After this time, some animals were mated with fertile males and some fertility parameters were assayed; the other animals were euthanized for preliminary toxicity parameters analysis. The effects of AEDv on the different stages of gestation were assayed on selected animals previously controlled for estrous cycle regularity and mated. AEDv led to an increase in serum, uterine and ovarian proteins as well as in ovarian and uterine weights (P < 0.05) in immature female rats. Hepatic proteins significantly decreased (P < 0.01) in high dose-treated animals (50 and 100 mg/kg) compared with controls. The number of implantation sites and the fertility rate were significantly lower (P < 0.05), while the antifertility activity increased significantly (P < 0.05) in treated rats compared with controls. When administered from the 1st to the 5th day of pregnancy, AEDv led to a decrease of more than 60% in the implantation rate in high dose-treated rats (50, 100, and 400 mg/kg). From the 6th to the 9th day, the implantation, gestation rates and the number of fetuses decreased significantly in all treated groups. From the 11th to the 20th day, a 50% resorption and decrease in gestation rate were reported in 50 mg/kg dose-treated animals. AEDv possesses weak contraceptive and abortifacient effects during pregnancy.

Some effects of the diet of mice on Nematospiroides dubius (Nematoda)

Parasitology ◽  
1969 ◽  
Vol 59 (1) ◽  
pp. 203-213 ◽  
Author(s):  
R. J. Bawden
Keyword(s):  
Technical Assistance ◽  
Growth Potential ◽  
The Other ◽  
Adequate Diet ◽  
Female Mice ◽  
Other Hand ◽  
Australian Wool ◽  
Nematospiroides Dubius

The establishment of N. dubius juveniles was more successful in mice maintained on a diet inadequate to support the full growth potential of the animals than in mice maintained on an adequate diet. The distribution of encysted juveniles along the duodenum was considerably more extensive in the former group of mice.More juveniles were established in male than in female mice within the respective dietary groups, although the pattern of distribution was similar.The distribution of adult nematodes at 10 days after infection was confined to a smaller area of the duodenum than that of the encysted juveniles at 5 days.The differences in distribution of nematodes after 5 and 10 days between the HP and LP mice was not evident after 21 days. The survival of adult nematodes in the HP mice was more successful than in the LP animals.The female nematodes in the LP mice achieved a greater length but were less prolific egg producers than those in the HP mice.A diet inadequate to support the full growth potential of mice was therefore associated with the increased ability of Nematospiroides dubius juveniles to establish themselves in mice compared with the situation in adequately fed animals. The low plane diet, on the other hand, was associated with a decreased ability of the adult nematodes to survive.This work was supported by grants from the Commonwealth Scholarship and Fellowship Plan and the Australian Wool Board. I should like to express my appreciation for the supervision of this work by Professor J. F. A. Sprent and to thank Miss Sharon McFeeter and Mrs Anne McKeown for their most competent technical assistance.

THE EFFECTS OF SPECIES, STRAIN AND SEX IN THYMOLYTIC TESTS OF BETAMETHASONE, DEXAMETHASONE AND OTHER STEROIDS

1962 ◽  
Vol 25 (1) ◽  
pp. 77-85 ◽  
Author(s):  
R. M. ATKINSON ◽  
M. A. PRATT ◽  
E. G. TOMICH
Keyword(s):  
Route Of Administration ◽  
Relative Potency ◽  
Male Mice ◽  
Female Mice ◽  
Betamethasone And Dexamethasone

SUMMARY Cortisol, prednisolone phosphate and the free alcohols and 21-phosphates of betamethasone and dexamethasone have been compared for thymolytic activity by the oral and subcutaneous routes in both sexes of two strains of rats and two strains of mice. The relative potency of betamethasone and dexamethasone differed with the route of administration and the sex, strain and species of animal employed. In female mice of the A2G strain, betamethasone was as potent as dexamethasone; in male mice of this strain, and in both sexes of GFF mice, WAG rats and PVG rats, betamethasone was much less potent than dexamethasone.

P–060 Dose- dependent mitigation of lead acetate toxicity in males on embryo development in female mice

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
P Dolati ◽  
M J Zamiri ◽  
A Akhlaghi ◽  
Z Jahromi
Keyword(s):  
Adverse Effects ◽  
Embryonic Development ◽  
Embryo Development ◽  
Lead Acetate ◽  
Endocrine System ◽  
Cell Number ◽  
The Other ◽  
Control Group ◽  
Male Mice ◽  
Female Mice

Abstract Study question Does quercetin (75 or 100 mg/kg BW/day) co-administration with lead acetate to male mice affects embryonic development in female mice? Summary answer The low-dose quercetin (75 mg/kg BW/day) ameliorated the adverse effects of lead acetate on mouse embryogenesis. What is known already Lead causes male infertility by impacting on endocrine system and spermatogenesis, and may exert undesirable effects on the offspring. The currently approved treatment for lead poisoning is the use of chelating agents, which form an insoluble complex with lead and shield it from biological targets; thus, reducing its toxicity. One of the main mechanisms of lead-induced toxicity is oxidative stress, and it has been reported that natural antioxidants can reduce the heavy metals toxicity. The aim of the present study was to examine the protective effects of quercetin on the toxicity induced by lead acetate on the embryogenesis in mice. Study design, size, duration Sexually mature (eight-week-old) NMRI male mice (n = 24) were randomly divided into four groups (n = 6 per group) receiving (i) distilled water (control group); (ii) lead acetate (150 mg/kg BW/day) dissolved in deionized water (LA); (iii) lead acetate (150 mg/kg BW/day) + quercetin (75 mg/kg BW/day) (LQ75); (IV) lead acetate (150 mg/kg BW/day) + quercetin (100 mg/kg BW/day) (LQ100). Treatments were applied daily as oral gavages for one cycle of the seminiferous epithelium (35 days). Participants/materials, setting, methods At the end of treatment administration, the males were joined with super-ovulated females, and the retrieved zygotes were cultured for evaluation of the embryo development (at 2-cell, 4-cell, 8-cell, and blastocyst stages), and blastocyst cell number using differential staining (propidium iodide and bisbenzimide). After incubation of capacitated sperm with oocytes, an ultraviolet light microscope was used following 3 min incubation with 25 µg⁄mL bisbenzamide solution for fertilization assessment. Main results and the role of chance Lead acetate (LA) treatment of male mice decreased the 2-cell stage compared with the control group (P &gt; 0.05). There was no difference between control and LQ75, and between LA and LQ100. The other stages of embryonic development were not significantly affected by the treatment. Overall, early embryonic development in the control and LQ75 mice were better than LQ100 and LA mice. The number of cells in the trophectoderm and inner-cell mass were not affected by treatments. However, the total blastocyst cell number in the control was higher than in the other groups; there was no significant difference between LQ100, LQ75 and LA groups. Fertilization rate was not affected by the treatments (P &lt; 0.05). Quercetin acts as a potent antioxidant at low doses, but at high doses exerts a pro-oxidant action. According to previous reports, higher concentrations of quercetin increased apoptosis and necrosis while decreasing the activities of the antioxidant enzymes. Also, it has been suggested that quercetin might disrupt the endocrine system and interfere with Sertoli cell function and sperm motility. Limitations, reasons for caution A limitation of this study is narrow dose selection; more studies are needed to determine the effective dose of quercetin in ameliorating the lead toxicity. There are also side effects of lead-quercetin chelates such as metal redistribution, essential metal loss, accumulation and persistency in intracellular sites, and peroxidation. Wider implications of the findings: Lead administration adversely impacted on the embryogenesis; on the other hand, paternal quercetin co-administration somewhat ameliorated the adverse effects of lead on mice embryogenesis. Trial registration number Not applicable

scholarly journals Studies on Amphibian Metamorphosis

1939 ◽  
Vol 16 (1) ◽  
pp. 96-120
Author(s):  
O. M. HELFF
Keyword(s):  
Great Majority ◽  
Gill Tissue ◽  
Shoulder Girdle ◽  
The Other ◽  
Histological Structure ◽  
Serial Sections ◽  
Stages Of Development ◽  
Amphibian Metamorphosis ◽  
The Right ◽  
Made In

(1) Opercular integument, homoplastically transplanted to the back and side of R. temporaria larvae, underwent a process of partial degeneration. The histolysis was not confined to any localized region of the transplant. (2) Autoplastic transplantation of opercular integument to the back and side produced a variety of results. Normal histological structure was maintained in certain transplants, generalized degeneration was observed in others, while in several instances localized histolysis resulting in perforation formation occurred. (3) Homoplastic and autoplastic transplantation of back and side skin to the opercular region resulted in histolysis and perforation formation in such transplants during larval involution. (4) The right forelimbs (in early stages of development) with attached portions of the shoulder girdle were extirpated in R. temporaria and B. bufo. During subsequent metamorphosis, normal opercular histolysis followed by perforation formation in many cases was observed. In most instances, serial sections of the peribranchial cavity revealed the absence of cutaneous glands. (5) Extirpation of the right forelimb only was made in the same two species. Opercular histolysis subsequently occurred in all instances, resulting in perforation formation in the great majority of cases during larval involution. In many of the B. bufo animals two separate perforations developed, one filled with limb stump and the other with gill tissue. (6) It is concluded that in R. temporaria a particular area of the operculum may in some individuals possess self-degenerative potentialities conducive to histolysis and perforation formation during metamorphosis. In both R. temporaria and B. bufo histolytic influences emanating from the atrophying gill tissue and the cutaneous glands of the forelimb are probably also responsible for opercular histolysis and perforation formation. Limb pressure must be considered a supplemental factor. (7) The results are discussed in general and attention called to the fact that opercular histolysis and perforation formation are "doubly assured" in some species and possibly even "triply assured" in others. Emphasis is placed on the evidently wide divergence between species as regards the particular combination of factors responsible for opercular histolysis and perforation formation. Apparently, no one explanation can serve to account for the phenomenon as it occurs in various species of anurans.

Disappearance of Meconium Pigment in Placental Specimens on Exposure to Light

2003 ◽  
Vol 127 (6) ◽  
pp. 711-714
Author(s):  
Jacquelyn L. Morhaime ◽  
Kay Park ◽  
Kurt Benirschke ◽  
Rebecca N. Baergen
Keyword(s):  
Neonatal Outcome ◽  
Light Exposure ◽  
Clinical Importance ◽  
Fetal Distress ◽  
Significant Loss ◽  
The Other ◽  
Serial Sections ◽  
Paired Samples ◽  
Weak Expression ◽  
Fluorescent Lighting

Abstract Context.—Meconium discharge has been associated with fetal distress and poor neonatal outcome; thus, its presence is of clinical importance. Objective.—Loss of meconium pigment in histologic sections from light exposure has been described. We sought to confirm this finding and to measure this loss quantitatively. Design.—Sections of umbilical cord, fetal membranes, and fetal surface from 11 grossly meconium-stained placentas were processed swiftly to minimize light exposure. Two serial sections from each block were cut and stained; one set was reviewed immediately, and the other was exposed to 8 hours of direct fluorescent lighting. Each site and exposure was scored for pigment intensity (0, no staining; 1, weak expression; and 2, moderate/strong expression) and number of meconium-laden macrophages per 10 high-power fields (HPF). Results were compared on the same specimen using the χ2 and the paired-samples t test. Results.—The maximum meconium macrophage count was 13.2/10 HPF in the unexposed sections versus 6.1/10 HPF in the exposed sections (P &lt; .001). Unexposed sections varied from 1+ to 2+ intensity, while exposed sections were all 1+ or negative (P &lt; .001). Conclusion.—Exposure to fluorescent laboratory lights for 8 hours resulted in a significant loss in the intensity and number of identifiable meconium macrophages in histologic sections. These findings have important implications in the handling of placental specimens, and we recommend that care be taken to minimize exposure to laboratory lights during processing.

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