scholarly journals Major Royal Jelly Proteins Accelerate Onset of Puberty and Promote Ovarian Follicular Development in Immature Female Mice

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1145-1145
Author(s):  
Lirong Shen ◽  
Xin Liu ◽  
Yong Chen ◽  
Fangxiong Shi ◽  
Chao-Qiang Lai
Keyword(s):  
Reproductive System ◽  
Royal Jelly ◽  
Hormone Secretion ◽  
Follicular Development ◽  
Serum Estradiol ◽  
Immature Female ◽  
Female Mice ◽  
Estrogenic Effect ◽  
Ovarian Follicular Development ◽  
Estradiol Levels

Abstract Objectives Estrogen plays an important role in for growth and development of reproductive system in female. The major royal jelly (RJ) has a significant estrogenic-like effect for many female animals. The major RJ proteins (MRJPs) are the central constituents responsible for the physiological activities of RJ. In recent years, we have found that MRJPs possesses functions to increase fecundity associated with estrogenic effect in Drosophila. However, if MRJPs are the specific active ingredient mediating estrogenic effect of RJ and related action mechanism in mammalians remains unclear. Methods Neonatal immature female mice (FC) were divided into four groups fed MRJPs with doses of 0, 125, 250 and 500 mg/kg/body weight (M125, M250 and M500), respectively. The freeze-dried MRJPs powder was administrated daily for 45 consecutive days. The effects of MRJPs on the puberty onset, ovarian follicular development and serum estradiol levels in FC were evaluated. Results The times of estrus in M125, M250 and M500 were accelerated by 10.7%, 15.5% and 10.7%, the ovarian index of M125 and M250 and M500 were increased by 26.8% and 32.1% and 1.7%. The number of secondary follicles in M125 and M250 and M500 were increased by 50.7%,78.8% and 38.6%, the Graafian follicles in M125 and M250 and M500 were increased by 600.0% and 774.0% and 150.0%. M500 induced multi-oocyte follicles. The serum estradiol levels of M125, M250 and M500 were increased by 47.1%, 64.9% and 31.1%, the action of MRJPs raising hormone secretion level is mainly via upregulating expression of ERβ gene. Antioxidant parameters of ovarian tissue showed that the malondialdehyde levels in M125 and M250 were decreased, the superoxide dismutase activities and glutathione peroxidase activities in M125 and M250 were increased, respectively. Conclusions Oral administration of MRJPs may accelerate onset of puberty and promote follicular development in immature FM. The reproductive performance promotion of MRJPs was associated with raising estrogenic activity and antioxidant potential to reproductive system, upregulating expression of ERβ gene to raise hormone secretion and promote ovary development in FM. Funding Sources The National Natural Science Foundation of China and US Department of Agriculture, Agriculture Research Service.

scholarly journals Major royal jelly proteins accelerate onset of puberty and promote ovarian follicular development in immature female mice

2020 ◽  
Vol 9 (4) ◽  
pp. 338-345
Author(s):  
Xin Liu ◽  
Chenmin Jiang ◽  
Yong Chen ◽  
Fangxiong Shi ◽  
Chaoqiang Lai ◽  
...  
Keyword(s):  
Royal Jelly ◽  
Follicular Development ◽  
Immature Female ◽  
Female Mice ◽  
Ovarian Follicular Development

119. THE EFFECT OF BMPR-IB IMMUNIZATION IN IMMATURE FEMALE MICE

2009 ◽  
Vol 21 (9) ◽  
pp. 38
Author(s):  
I. M. Ciller ◽  
J. D. McFarlane ◽  
J. R. McFarlane
Keyword(s):  
Reproductive System ◽  
Follicular Development ◽  
Significant Degree ◽  
Type I ◽  
Female Reproductive System ◽  
Immature Female ◽  
Ovarian Weight ◽  
Female Mice ◽  
Signaling Components

The female reproductive system is regulated by well known endocrine signaling components and the less well understood autocrine and paracrine signaling components by growth factors and their receptors. The type I Bone Morphogenetic Protein Receptor IB (BMPR-IB) is a serine/threonine kinase that facilitates the signaling of various TGF-β family members. BMPR-IB is believed to have a substantial role in reproduction as demonstrated by the hyperprolific effect on ovulation in Booroola ewes which harbor a natural BMPR-IB receptor mutation, as well as the sterility induced by the BMPR-IB knockout in female mice. Maturation of the ovary and enhancement of follicular growth are known to be dependent on gonadotrophin stimulation from the pituitary. This study aims to elucidate the role of BMPR-IB in the female reproductive system by passive immune neutralization. Immature female mice (21 days old) were passively immunized against BMPR-IB via subcutaneous injections of 100 μl PBS containing anti BMPR-IB Ig in the absence and presence of equine chorionic gonadotrophin (eCG). The preparations where administered on day one and three of a four day experiment. On day four mice were asphyxiated with CO2 and the ovaries were removed and weighed. In immature mice immunization against BMPR-IB ovarian weights were not different from control, while eCG significantly increased ovarian weight compared to the controls. In combination immunization against BMPR-IB further augmented the gonadotrophin-stimulated increase in ovarian weight to a significant degree. The suppressive effect of BMPR-IB signaling on follicular development in response to gonadotrophin stimulation indicates a possible developmental role of this receptor in the initiation of follicle growth in response to increased circulatory gonadotrophins and prevention of precocious puberty.

scholarly journals Effects of hypothyroidism on ovarian hormone secretion and follicular development in immature female rats

1997 ◽  
Vol 73 ◽  
pp. 103
Author(s):  
Minoru Hatsula ◽  
Kazuhiro Tamura ◽  
Gen Watanabe ◽  
Kazuyoshi Taya ◽  
Hiroshi Kogo Kogo
Keyword(s):  
Hormone Secretion ◽  
Follicular Development ◽  
Female Rats ◽  
Ovarian Hormone ◽  
Immature Female

Early ovarian follicular development in prepubertal Wistar rats acutely exposed to androgens

2016 ◽  
Vol 7 (4) ◽  
pp. 384-390 ◽  
Author(s):  
L. Paixão ◽  
L. M. Velez ◽  
B. R. Santos ◽  
C. Tusset ◽  
S. B. Lecke ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Follicular Development ◽  
Control Group ◽  
Female Wistar Rats ◽  
Ovarian Follicular Development ◽  
Morphology And Morphometry ◽  
Equine Chorionic Gonadotropin ◽  
Reproductive Consequences ◽  
And Control ◽  
Estradiol Levels

Androgens may directly modulate early ovarian follicular development in preantral stages and androgen excess before puberty may disrupt this physiological process. Therefore, the aim of this study was to investigate the dynamics of follicular morphology and circulating androgen and estradiol levels in prepubertal Wistar rats acutely exposed to androgens. Prepubertal female Wistar rats were distributed into three groups: control, equine chorionic gonadotropin (eCG) intervention and eCG plus dehydroepiandrosterone (DHEA) intervention (eCG+DHEA). Serum DHEA, testosterone and estradiol levels were determined, and ovarian morphology and morphometry were assessed. The eCG+DHEA group presented increased serum estradiol and testosterone levels as compared with the control group (P<0.01), and higher serum DHEA concentration v. the eCG-only and control groups (P<0.01). In addition, the eCG+DHEA group had a higher number of, and larger-sized, primary and secondary follicles as compared with the control group (P<0.05). The eCG group presented intermediate values for number and size of primary and secondary follicles, without significant differences as compared with the other two groups. The number of antral follicles was higher in the eCG+DHEA and eCG groups v. controls (P<0.05). The number of primordial, atretic and cystic follicles were similar in all groups. In conclusion, the present experimental model using an acute eCG+DHEA intervention was useful to investigate events involved in initial follicular development under hyperandrogenic conditions, and could provide a reliable tool to study defective follicular development with possible deleterious reproductive consequences later in life.

scholarly journals Hypercaloric diets impair ovarian follicular development by inducing hyperinsulinemia and hyperlipidemia in female mice

2020 ◽  
Author(s):  
Qiaoli Zhang ◽  
Yan Wang ◽  
Jiansheng Liu ◽  
Zi-Jiang Chen ◽  
Yanzhi Du
Keyword(s):  
Caspase 3 ◽  
Follicular Development ◽  
High Fat ◽  
Estrous Cycles ◽  
Akt Phosphorylation ◽  
Ovarian Follicular Development ◽  
Organelle Morphology ◽  
Related Proteins ◽  
Estradiol Levels

Abstract Long-term hypercaloric diets adversely impact ovarian follicular development and fecundity. We investigated the effects of high sugar (HS), high fat low sugar (HFLS), and high fat normal sugar (HFNS) diets on ovarian follicular development by feeding mice for up to 180 days. Body weight, gonadal fat, glucose, lipid, insulin, estrous cycle, sex hormones, ovarian tissues, and follicle ultrastructure were examined, and the expression of metabolism-related proteins was evaluated immunoblotting in ovarians. The mice on hypercaloric diets showed hyperinsulinemia and hyperlipidemia and exhibited heavier body and gonadal fat weights, longer estrous cycles, and fewer numbers of preantral and antral follicles; and the follicles that did form had impaired organelle morphology. The sex hormone levels in blood were similar to controls, excepting significantly elevated estradiol levels for the HS diet. In ovarian tissues, AMPKα phosphorylation was reduced while AKT phosphorylation and caspase-3 were increased in ovarian tissues in mice on all three hypercaloric diets. The data from our study collectively indicates possible mechanisms through which long-term exposure to unhealthy hypercaloric diets may impair ovarian follicular development: hyperinsulinemia and hyperlipidemia.

HUMAN URINARY GONADOTROPHINS ASSAYED BY DIFFERENT RESPONSES IN THE SAME ANIMAL

1960 ◽  
Vol XXXIV (III) ◽  
pp. 375-389 ◽  
Author(s):  
P. S. Brown
Keyword(s):  
Postmenopausal Women ◽  
Ovarian Response ◽  
The Other ◽  
Relative Potency ◽  
Vaginal Opening ◽  
Serial Sections ◽  
Immature Female ◽  
Female Mice ◽  
Marked Disparity ◽  
Relative Potencies

ABSTRACT Selected human urinary gonadotrophins were assayed against one another using various measures of response in the same immature female mice. Intact or hypophysectomized animals were used and in some experiments the results of hypophysectomy were checked in complete serial sections. Extracts from the urine of two subjects with Turner's syndrome were compared. In intact mice, the relative potency judged by the ovarian response differed from that shown by the uterine response and the 95 % fiducial limits of the two estimates did not overlap. When the mice were hypophysectomized, one extract became much less potent while the other did not. Similar differences were shown in the response of intact mice to urinary extracts from two subjects with Klinefelter's syndrome. There was a marked disparity between the relative potencies shown by the uterine response and by the incidence of vaginal opening. Similar differences were not shown between the responses to different extracts from the urine of normal postmenopausal women, but these extracts were known to differ little in quality. The results are interpreted in terms of qualitative differences between human urinary gonadotrophins.

PHYSICAL DISORDERS AFTER HYSTERECTOMY DEPEND ON MRS AND SERUM ESTRADIOL LEVELS

2016 ◽  
pp. 41-45
Author(s):  
Lam Huong Le
Keyword(s):  
Key Words ◽  
Cross Sectional Study ◽  
Sexual Disorders ◽  
Serum Estradiol ◽  
Sectional Study ◽  
Cross Sectional ◽  
Total Hysterectomy ◽  
Physical Disorders ◽  
The Relationship ◽  
Estradiol Levels

Objectives: To study the relationship between serum estradiol and physical disorders after hysterectomy. Methods: A descriptive cross sectional study on 151 women after total hysterectomy from 12/2008 to 11/2010 at Hue Cental Hospial. Results: Serious disorders in total hysterectomy group include: physical disorders (34.78%), maternal disorders (71.74%), sexual disorders (58.70%). There were significant differences these disorders between total hysterectomy group and hysterectomy with/without accessiory remove group, as well as menopause group. Conclusion: Serum estradiol levels was inversely correlated with physical disorders, martenal disorders, sexual disorders and other disorders. Key words: hysterectomy, serum estradiol

Can serum estradiol levels on the fourth day of IVF/ICSI cycle predict outcome in poor responder women?

2015 ◽  
Vol 61 (4) ◽  
pp. 233-237 ◽  
Author(s):  
Serkan Kahyaoglu ◽  
Omer H. Yumusak ◽  
A. Seval Ozgu-Erdinc ◽  
Saynur Yilmaz ◽  
Inci Kahyaoglu ◽  
...  
Keyword(s):  
Poor Responder ◽  
Serum Estradiol ◽  
Icsi Cycle ◽  
Estradiol Levels
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