Purification and characterization of α2-macroglobulin from grass carp Ctenopharyngodon idellus: Cloning a segment of the corresponding gene

2006 ◽  
Vol 20 (4) ◽  
pp. 474-481 ◽  
Author(s):  
F.L. Li ◽  
C.P. Lu
Keyword(s):  
Grass Carp ◽  
Ctenopharyngodon Idellus ◽  
Purification And Characterization ◽  
Α2 Macroglobulin

Isolation and genetic characterization of Flavobacterium columnare from grass carp, Ctenopharyngodon idellus, in China

Aquaculture ◽  
2021 ◽  
Vol 541 ◽  
pp. 736762
Author(s):  
Zijun Lu ◽  
Ren Gao ◽  
Yafei Duan ◽  
Rui Han ◽  
Wenjie Guo ◽  
...  
Keyword(s):  
Grass Carp ◽  
Genetic Characterization ◽  
Flavobacterium Columnare ◽  
Ctenopharyngodon Idellus

Purification and Characterization of Kallikrein from Plasma of Patients with Acute Pancreatitis

1981 ◽  
Vol 60 (2) ◽  
pp. 199-205 ◽  
Author(s):  
Naotika Toki ◽  
Hiroyuki Sumi ◽  
Sumiyoshi Takasugi
Keyword(s):  
Acute Pancreatitis ◽  
Specific Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Apparent Molecular Weight ◽  
Purification And Characterization ◽  
Α2 Macroglobulin ◽  
Sepharose 4B

1. A kallikrein-like enzyme in plasma of patients with acute pancreatitis was further purified by successive hydroxyapatite/cellulose and Sepharose-4B column chromatography. 2. By these procedures 0.26 mg of purified enzyme with a specific activity of 215 S-2266 chromozyme units/mg of protein was obtained from 10 ml of original plasma. 3. The purified material was homogeneous as ascertained by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and had an apparent molecular weight of 31 000 as measured by gel filtration on Sephadex G-200. 4. It was confirmed immunologically that this enzyme was pancreatic kallikrein, which is distinct from plasma kallikrein, and that it could combine with α2-macroglobulin only in the presence of trypsin.

Construction and Characterization of Grass Carp (Ctenopharyngodon idellus) Fosmid Library

2010 ◽  
Vol 9 (9) ◽  
pp. 1347-1352
Author(s):  
Zhen-hu JIA ◽  
Chang-you LIN ◽  
Tian-yao YANG ◽  
Yi-nan JIANG ◽  
Chun XIA
Keyword(s):  
Grass Carp ◽  
Fosmid Library ◽  
Ctenopharyngodon Idellus
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