Temperature-induced transcription of inflammatory mediators and the influence of Hsp70 following LPS stimulation of southern bluefin tuna peripheral blood leukocytes and kidney homogenates

Exposure of esophageal mucosa to hydrochloric acid (HCl) is a crucial factor in the pathogenesis of reflux disease. We examined supernatant of HCl-exposed rabbit mucosa for inflammatory mediators enhancing migration of leukocytes and production of H2O2 as an indicator of leukocyte activation. A tubular segment of rabbit esophageal mucosa was tied at both ends to form a sac, which was filled with HCl-acidified Krebs buffer at pH 5 (or plain Krebs buffer as control) and kept oxygenated at 37°C. The medium around the sac (supernatant) was collected after 3 h. Rabbit peripheral blood leukocytes (PBL) were isolated, and sac supernatant was used to investigate PBL migration and H2O2 production. HCl-exposed esophageal mucosa released substance P (SP), CGRP, platelet-activating factor (PAF), and IL-8 into the supernatant. PBL migration increased in response to IL-8 or to supernatant of the HCl-filled mucosal sac. Supernatant-induced PBL migration was inhibited by IL-8 antibodies and by antagonists for PAF (CV3988) or neurokinin 1 (i.e., SP), but not by a CGRP antagonist. Supernatant of the HCl-filled mucosal sac increased H2O2 release by PBL that was significantly reduced by CV3988 and by a SP antagonist but was not affected by IL-8 antibodies or by a CGRP antagonist. We conclude that IL-8, PAF, and SP are important inflammatory mediators released by esophageal mucosa in response to acid that promote PBL migration. In addition, PAF and SP induce production of H2O2 by PBL. These findings provide a direct link between acid exposure and recruitment and activation of immune cells in esophageal mucosa.

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Peanut agglutinin (PNA): Binding and stimulation of bovine intestinal and peripheral blood leukocytes

Veterinary Immunology and Immunopathology ◽

10.1016/0165-2427(89)90164-5 ◽

1989 ◽

Vol 22 (1) ◽

pp. 67-78 ◽

Cited By ~ 6

Author(s):

A.M. Nagi ◽

L.A. Babiuk

Keyword(s):

Peripheral Blood ◽

Peripheral Blood Leukocytes ◽

Peanut Agglutinin ◽

Blood Leukocytes ◽

Stimulation Of

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ω-3 PUFAs and Resveratrol Differently Modulate Acute and Chronic Inflammatory Processes

BioMed Research International ◽

10.1155/2015/535189 ◽

2015 ◽

Vol 2015 ◽

pp. 1-11 ◽

Cited By ~ 12

Author(s):

Joseph Schwager ◽

Nathalie Richard ◽

Christoph Riegger ◽

Norman Salem

Keyword(s):

Gene Expression ◽

Chronic Inflammation ◽

Inflammatory Mediators ◽

Peripheral Blood ◽

Acute Inflammation ◽

Synergistic Effects ◽

Peripheral Blood Leukocytes ◽

Blood Leukocytes

ω-3 PUFAs and polyphenols have multiple effects on inflammationin vivoandin vitro. The effects of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and resveratrol (RV) were investigated in LPS-stimulated peripheral blood leukocytes (PBLs) (i.e., acute inflammation) and IL-1βactivated human chondrocytes (i.e., chronic inflammation). Inflammatory mediators including chemokines, cytokines, interleukins, and PGE2were measured by multiplex analysis and gene expression was quantified by RT-PCR. In PBLs, RV decreased the secretion of PGE2, CCL5/RANTES, and CXCL8/IL-8 but increased IL-1β, IL-6, and IL-10. In contrast to RV,ω-3 PUFAs augmented the production of PGE2and CXCL8/IL-8. EPA and DHA similarly affected the pattern of inflammatory mediators. Combination of RV andω-3 PUFAs exerted synergistic effects on CCL5/RANTES and had additive effects on IL-6 or CXCL8/IL-8. Bothω-3 PUFAs and RV reduced catabolic gene expression (e.g., MMPs, ADAMTS-4, IL-1β, and IL-6) in activated chondrocytes. The data suggest thatω-3 PUFAs and RV differ in the regulation of acute inflammation of peripheral blood leukocytes but have common properties in modulating features related to chronic inflammation of chondrocytes.

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