INFLUENCE OF A PERIOPERATIVE NUTRITION ENRICHED WITH OMEGA-3 FATTY ACIDS ON THE SYNTHESIS OF INFLAMMATORY MEDIATORS FROM PERIPHERAL BLOOD LEUKOCYTES IN PATIENTS WITH MAJOR SURGERY.

AbstractLimited data are available evaluating the effects of omega-3 fatty acids supplementation on gene expression involved in the insulin and lipid-signaling pathway in women with polycystic ovary syndrome (PCOS). This study was conducted to evaluate the effects of omega-3 fatty acids supplementation on gene expression involved in the insulin and lipid signaling pathway in women with PCOS. This randomized double blind, placebo-controlled trial was done among 60 women aged 18–40 years old and diagnosed with PCOS according to the Rotterdam criteria. Participants were randomly assigned into 2 groups to receive either 1 000 mg omega-3 fatty acids from flaxseed oil containing 400 mg α-linolenic acid (n=30) or placebo (n=30) twice a day for 12 weeks. Gene expressions involved in the insulin and lipid-signaling pathway were quantified in blood samples of PCOS women with RT-PCR method. Quantitative results of RT-PCR demonstrated that compared with the placebo, omega-3 fatty acids supplementation upregulated peroxisome proliferator-activated receptor gamma (PPAR-γ) mRNA (p=0.005) in peripheral blood mononuclear cells of women with PCOS. In addition, compared to the placebo, omega-3 fatty acids supplementation downregulated expressed levels of oxidized low-density lipoprotein receptor (LDLR) mRNA (p=0.002) in peripheral blood mononuclear cells of women with PCOS. We did not observe any significant effect of omega-3 fatty acids supplementation on expressed levels of glucose transporter 1 (GLUT-1) and lipoprotein(a) [Lp(a)] genes in peripheral blood mononuclear cells. Overall, omega-3 fatty acids supplementation for 12 weeks in PCOS women significantly improved gene expression of PPAR-γ and LDLR.

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In vitro effects of nonesterified fatty acids and β-hydroxybutyric acid on inflammatory cytokine expression in bovine peripheral blood leukocytes

Italian Journal of Animal Science ◽

10.1080/1828051x.2021.2006806 ◽

2021 ◽

Vol 20 (1) ◽

pp. 2197-2210

Author(s):

Cheng-Yan Li ◽

Yu-Wen Liao ◽

Chia-So Liu ◽

Chuen-Yu Cheng ◽

Jacky Peng-Wen Chan ◽

...

Keyword(s):

Fatty Acids ◽

Peripheral Blood ◽

Inflammatory Cytokine ◽

Cytokine Expression ◽

Peripheral Blood Leukocytes ◽

Hydroxybutyric Acid ◽

Blood Leukocytes ◽

Nonesterified Fatty Acids ◽

In Vitro Effects

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Acute and chronic omega-3 fatty acids activates Gpr120 in peripheral blood mononuclear cells from nafld and obese patients reducing inflammation

Clinical Nutrition ESPEN ◽

10.1016/j.clnesp.2020.09.748 ◽

2020 ◽

Vol 40 ◽

pp. 654

Author(s):

E. Batista ◽

V. Muñoz ◽

J. Jesus ◽

M. Vasconcelos ◽

J.M. Rocha ◽

...

Keyword(s):

Fatty Acids ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Obese Patients ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

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HCl-induced inflammatory mediators in esophageal mucosa increase migration and production of H2O2 by peripheral blood leukocytes

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00160.2010 ◽

2010 ◽

Vol 299 (3) ◽

pp. G791-G798 ◽

Cited By ~ 17

Author(s):

Jie Ma ◽

Annamaria Altomare ◽

Suzanne de la Monte ◽

Ming Tong ◽

Florian Rieder ◽

...

Keyword(s):

Inflammatory Mediators ◽

Peripheral Blood ◽

Platelet Activating Factor ◽

Esophageal Mucosa ◽

Leukocyte Activation ◽

Peripheral Blood Leukocytes ◽

Blood Leukocytes ◽

Neurokinin 1 ◽

Krebs Buffer ◽

Cgrp Antagonist

Exposure of esophageal mucosa to hydrochloric acid (HCl) is a crucial factor in the pathogenesis of reflux disease. We examined supernatant of HCl-exposed rabbit mucosa for inflammatory mediators enhancing migration of leukocytes and production of H2O2 as an indicator of leukocyte activation. A tubular segment of rabbit esophageal mucosa was tied at both ends to form a sac, which was filled with HCl-acidified Krebs buffer at pH 5 (or plain Krebs buffer as control) and kept oxygenated at 37°C. The medium around the sac (supernatant) was collected after 3 h. Rabbit peripheral blood leukocytes (PBL) were isolated, and sac supernatant was used to investigate PBL migration and H2O2 production. HCl-exposed esophageal mucosa released substance P (SP), CGRP, platelet-activating factor (PAF), and IL-8 into the supernatant. PBL migration increased in response to IL-8 or to supernatant of the HCl-filled mucosal sac. Supernatant-induced PBL migration was inhibited by IL-8 antibodies and by antagonists for PAF (CV3988) or neurokinin 1 (i.e., SP), but not by a CGRP antagonist. Supernatant of the HCl-filled mucosal sac increased H2O2 release by PBL that was significantly reduced by CV3988 and by a SP antagonist but was not affected by IL-8 antibodies or by a CGRP antagonist. We conclude that IL-8, PAF, and SP are important inflammatory mediators released by esophageal mucosa in response to acid that promote PBL migration. In addition, PAF and SP induce production of H2O2 by PBL. These findings provide a direct link between acid exposure and recruitment and activation of immune cells in esophageal mucosa.

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Omega 3 Fatty Acids Counteract IL-8 and Prostaglandin E2 Secretion Induced by TNF-α in Cultured Endometrial Stromal Cells

Journal of Endometriosis ◽

10.5301/je.2011.8331 ◽

2011 ◽

Vol 3 (1) ◽

pp. 34-39

Author(s):

Romina Novembri ◽

Stefano Luisi ◽

Patrizia Carrarelli ◽

Valentina Ciani ◽

Flavio de Pascalis ◽

...

Keyword(s):

Fatty Acids ◽

Inflammatory Mediators ◽

Stromal Cells ◽

Inhibitory Effect ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Endometrial Stromal Cells ◽

Tnf Α ◽

Key Enzyme ◽

Cox 2

Background Inflammation is part of the normal host response to infection and injury and excess inflammation may lead to acute or chronic diseases. Endometriosis is a common gynecologic disease characterized by increased endometrial production of inflammatory mediators such as cytokines, reactive oxygen species, and prostaglandins. Omega-3 polyunsaturated fatty acids play a role in the inflammatory process and a therapeutic effect has been suggested. Methods On this basis, this study aimed to assess in human cultured endometrial stromal cells the effect of two omega-3 fatty acids (DHA and EPA), on i) TNF-α induced IL-8 and PGE2 release and on ii) cyclooxygenase-2 (Cox-2) mRNA expression a key enzyme for prostaglandin production. Results DHA or EPA significantly reduced LPS-induced IL-8 (P<0.01) and PGE2 (P<0.01) release decreasing the expression of Cox-2 (P<0.01). Conclusions In conclusion, our study revealed an inhibitory effect of omega-3 fatty acids on inflammatory mediators secretion and suggests a potential benefit for the treatment of pelvic pain in patients with dysmenorrhea and/or endometriosis.

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Enriched Marine Oil Supplements Increase Peripheral Blood Specialized Pro-Resolving Mediators Concentrations and Reprogram Host Immune Responses

Circulation Research ◽

10.1161/circresaha.119.315506 ◽

2020 ◽

Vol 126 (1) ◽

pp. 75-90 ◽

Cited By ~ 22

Author(s):

Patricia R. Souza ◽

Raquel M. Marques ◽

Esteban A. Gomez ◽

Romain A. Colas ◽

Roberta De Matteis ◽

...

Keyword(s):

Fatty Acids ◽

Peripheral Blood ◽

Essential Fatty Acids ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Marine Oil ◽

Protective Actions ◽

The Relationship ◽

Dose Dependent Increase ◽

Dose Dependent

Rationale: Specialized pro-resolving mediators (SPM—lipoxins, resolvins, protectins, and maresins) are produced via the enzymatic conversion of essential fatty acids, including the omega-3 fatty acids docosahexaenoic acid and n-3 docosapentaenoic acid. These mediators exert potent leukocyte directed actions and control vascular inflammation. Supplementation of animals and humans with essential fatty acids, in particular omega-3 fatty acids, exerts protective actions reducing vascular and systemic inflammation. Of note, the mechanism(s) activated by these supplements in exerting their protective actions remain poorly understood. Objective: Given that essential fatty acids are precursors in the biosynthesises of SPM, the aim of the present study was to establish the relationship between supplementation and peripheral SPM concentrations. We also investigated the relationship between changes in plasma SPM concentrations and peripheral blood platelet and leukocyte responses. Methods and Results: Healthy volunteers were enrolled in a double-blinded, placebo-controlled, crossover study, and peripheral blood was collected at baseline, 2, 4, 6, and 24 hours post administration of placebo or one of 3 doses of an enriched marine oil supplement. Assessment of plasma SPM concentrations using lipid mediator profiling demonstrated a time- and dose-dependent increase in peripheral blood SPM concentration. Supplementation also led to a regulation of peripheral blood cell responses. Here we found a dose-dependent increase in neutrophil and monocyte phagocytosis of bacteria and a decrease in the diurnal activation of leukocytes and platelets, as measured by a reduction in adhesion molecule expression. In addition, transcriptomic analysis of peripheral blood cells demonstrated a marked change in transcript levels of immune and metabolic genes 24 hours post supplementation when compared with placebo. Conclusions: Together, these findings demonstrate that supplementation with an enriched marine oil leads to an increase in peripheral blood SPM concentrations and reprograms peripheral blood cells, indicating a role for SPM in mediating the immune-directed actions of this supplement. Clinical Trial Registration: URL: http://www.clinicaltrials.gov . Unique identifier: NCT03347006.

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