High density lipoproteins down-regulate transcriptional expression of pro-inflammatory factors and oxidative burst in head kidney leukocytes from rainbow trout, Oncorhynchus mykiss

2013 ◽  
Vol 35 (1) ◽  
pp. 180-183 ◽  
Author(s):  
Franz Villarroel ◽  
Alin Casado ◽  
Rodolfo Amthauer ◽  
Margarita I. Concha
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Oxidative Burst ◽  
Head Kidney ◽  
High Density ◽  
Inflammatory Factors ◽  
High Density Lipoproteins ◽  
Transcriptional Expression ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals Optimisation of the lymphocyte proliferation assay in rainbow trout (Oncorhynchus mykiss)

2019 ◽  
Vol 64 (No. 12) ◽  
pp. 547-557
Author(s):  
H Minarova ◽  
M Palikova ◽  
J Mares ◽  
E Syrova ◽  
J Blahova ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Bovine Serum ◽  
Lymphocyte Proliferation ◽  
Head Kidney ◽  
Pokeweed Mitogen ◽  
Lymphocyte Proliferation Assay ◽  
Proliferation Assay ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Foetal Bovine Serum

The lymphocyte proliferation assay is a valuable method used for the evaluation of the fish immune system. However, there are many variations and optimal results are not always obtained. Unification is necessary to ensure the comparability between different studies. The aim of this study was to optimise the lymphocyte proliferation assay in rainbow trout (Oncorhynchus mykiss). This goal included the determination of the optimal incubation length, serum type, incubation temperature, type of mitogen and its concentration, and anticoagulant. The peripheral blood and head kidney lymphocytes were isolated by density gradient centrifugation. Subsequently, the cells were incubated for 3–8 days with different mitogens (pokeweed mitogen 5, 10 and 50 µg/ml, concanavalin A 1, 10 and 20 µg/ml, phytohaemagglutinin 25, 50 and 100 µg/ml, lipopolysaccharide 1, 50 and 100 µg/ml). The use of the different serum types (foetal bovine serum, trout serum), incubation temperatures (10–20 °C) and anticoagulants (heparin, EDTA) was compared. Labelled thymidine was used to evaluate the assay. The best results were obtained after seven days of incubation at 15 °C with foetal bovine serum (FBS). The head kidney lymphocytes showed the highest proliferative response with 50 µg/ml phytohaemagglutinin. With the peripheral blood lymphocytes (heparin and EDTA), the best results were obtained with 50 µg/ml pokeweed mitogen. The highest proliferation levels were detected with heparinised blood. In conclusion, optimisation of this assay contributes to the improved assessment of the rainbow trout immune function.

Transcriptomic responses to heat stress in rainbow trout Oncorhynchus mykiss head kidney

2018 ◽  
Vol 82 ◽  
pp. 32-40 ◽  
Author(s):  
Jinqiang Huang ◽  
Yongjuan Li ◽  
Zhe Liu ◽  
Yujun Kang ◽  
Jianfu Wang
Keyword(s):  
Heat Stress ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Head Kidney ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Transcriptomic Responses

scholarly journals 11-Deoxycorticosterone Is a Potent Agonist of the Rainbow Trout (Oncorhynchus mykiss) Mineralocorticoid Receptor

Endocrinology ◽  
2005 ◽  
Vol 146 (1) ◽  
pp. 47-55 ◽  
Author(s):  
A. Sturm ◽  
N. Bury ◽  
L. Dengreville ◽  
J. Fagart ◽  
G. Flouriot ◽  
...  
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Mineralocorticoid Receptor ◽  
Head Kidney ◽  
Luciferase Reporter ◽  
Similar Response ◽  
Hormonal Stimulation ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Juvenile Trout

The teleost fish are thought to lack the mineralocorticoid hormone aldosterone but possess mineralocorticoid receptor (MR) homologs. Here we describe the characterization of two rainbow trout (Oncorhynchus mykiss) MRs, called rtMRa and rtMRb. The open reading frame of rtMRa cDNA encoded a protein of 1041 amino acids. The rtMRb predicted protein sequence is similar, differing in only 10 amino acids in the nonconserved A/B domain and lacking a three-amino acid insertion between the two zinc fingers of the C domain. Expression of rtMR mRNA (sum of both forms), measured in juvenile trout by real-time RT-PCR, shows that the transcripts are ubiquitous. Expression was significantly higher in brain than the other tissues studied (eye, trunk kidney, head kidney, gut, gills, liver, spleen, ovary, heart, white muscle, skin). Hormonal stimulation of receptor transactivation activity was studied in COS-7 cells transiently cotransfected with receptor cDNA and a mouse mammary tumor virus-luciferase reporter. The mineralocorticoids 11-deoxycorticosterone and aldosterone were more potent enhancers of rtMRa transcriptional activity (EC50 = 1.6 ± 0.5 × 10−10 and 1.1 ± 0.4 × 10−10m, respectively) than the glucocorticoids cortisol and 11-deoxycortisol (EC50 = 1.1 ± 0.3 × 10−9 and 3.7 ± 1.9 × 10−9m, respectively). A similar response was observed in transactivation assays with rtMRb. These results are discussed in the view of reported circulating levels of corticosteroids in trout.

scholarly journals Draft Genome Sequence of Epilithonimonas sp. FP211-J200, Isolated from an Outbreak Episode on a Rainbow Trout (Oncorhynchus mykiss) Farm

2017 ◽  
Vol 5 (37) ◽  
Author(s):  
Manuel Ayala ◽  
Cristopher Segovia ◽  
Rodrigo Rojas ◽  
Claudio Miranda ◽  
Javier Santander
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Genome Sequence ◽  
Draft Genome ◽  
Head Kidney ◽  
Fish Host ◽  
Draft Genome Sequence ◽  
Kidney Cells ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Head Kidney Cells

ABSTRACT Here, we report the draft genome sequence of Epilithonimonas sp. FP211-J200, isolated from rainbow trout head kidney cells. The size of the genome is 4,110,772 bp, with a G+C content of 37.1%. The Epilithonimonas sp. FP211-J200 genome has genes related to tetracycline and β-lactam resistance. This is the first reported Epilithonimonas species genome isolated from a fish host.

Characterisation of the binding of leukotriene B4 to macrophages of the rainbow trout Oncorhynchus mykiss.

1997 ◽  
Vol 200 (1) ◽  
pp. 155-160
Author(s):  
L A Bowden ◽  
S Fiore ◽  
C J Restall ◽  
C N Serhan ◽  
A F Rowley
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Receptor Antagonist ◽  
Head Kidney ◽  
Leukotriene B4 ◽  
Scatchard Analysis ◽  
Single Class ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Kinetics Of ◽  
Dissociation Constant Kd

The binding of leukotriene B4 (LTB4) to macrophages from the head kidney of the rainbow trout Oncorhynchus mykiss was measured. Binding of [3H]LTB4 achieved a steady state after approximately 30 min of incubation and was 30% reversible in the presence of a minimum of 1000-fold excess of LTB4. Scatchard analysis of the kinetics of LTB4 binding over a range of [3H]LTB4 concentrations indicated the existence of only a single class of receptor with a dissociation constant, KD, of 0.14 nmol l-1 and a maximum receptor density, Bmax, of approximately 17,800 sites per macrophage. The LTB4 receptor antagonist LY223982 was ineffective in inhibiting the binding of [3H]LTB4 to trout macrophages, although another receptor antagonist, LTB4-dimethylamide, displaced a maximum of 25% of the total binding. LTB5 was equally effective as LTB4 at displacing [3H]LTB4, while other eicosanoids tested were without significant effect. It is suggested that the putative receptors for LTB4 on trout macrophages are similar to the high-affinity receptors for this compound reported to occur on mammalian granulocytes, although any structural similarities of the binding sites await further investigation.

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