Functional characterization of purinergic P2Y2 and P2Y12 receptors involved in Japanese flounder (Paralichthys olivaceus) innate immune responses

2018 ◽  
Vol 75 ◽  
pp. 208-215 ◽  
Author(s):  
Shuo Li ◽  
Gaixiang Hao ◽  
Yaqi Xu ◽  
Nan Wang ◽  
Jiafang Li ◽  
...  
Keyword(s):  
Immune Responses ◽  
Paralichthys Olivaceus ◽  
Functional Characterization ◽  
Japanese Flounder ◽  
Innate Immune ◽  
Innate Immune Responses

scholarly journals Extracellular ATP is a potent signaling molecule in the activation of the Japanese flounder (Paralichthys olivaceus) innate immune responses

2020 ◽  
Vol 26 (5) ◽  
pp. 413-423 ◽  
Author(s):  
Shuo Li ◽  
Xiaoli Chen ◽  
Jiafang Li ◽  
Xuejing Li ◽  
Tianxu Zhang ◽  
...  
Keyword(s):  
Immune Responses ◽  
Immune Cells ◽  
Inflammatory Cytokine ◽  
Paralichthys Olivaceus ◽  
Head Kidney ◽  
Japanese Flounder ◽  
Innate Immune ◽  
Kidney Cells ◽  
Innate Immune Responses ◽  
Head Kidney Cells

Innate immunity is the first line of defense against pathogen infections. Extracellular ATP (eATP) is one of the most studied danger-associated molecular pattern molecules that can activate host innate immune responses through binding with and activating purinergic receptors on the plasma membrane. The detailed actions of eATP on fish innate immunity, however, remain poorly understood. In this study, we investigated bacterial pathogen-induced ATP release in head kidney cells of the Japanese flounder Paralichthys olivaceus. We also examined the actions of eATP on pro-inflammatory cytokine and immune-related gene expression, the activity of induced NO synthase (iNOS), and the production of reactive oxygen species (ROS) and NO in Japanese flounder immune cells. We demonstrate that ATP is dynamically released from Japanese flounder head kidney cells into the extracellular milieu during immune challenge by formalin-inactivated Edwardsiella tarda and Vibrio anguillarum. In addition, we show that eATP administration results in profound up-regulation of pro-inflammatory cytokine gene expression, iNOS activity, and inflammatory mediator production, including ROS and NO, in Japanese flounder immune cells. Altogether, our findings demonstrate that eATP is a potent signaling molecule for the activation of innate immune responses in fish.

scholarly journals Characterization of a long-term mouse primary liver 3D tissue model recapitulating innate-immune responses and drug-induced liver toxicity

PLoS ONE ◽  
2020 ◽  
Vol 15 (7) ◽  
pp. e0235745 ◽  
Author(s):  
Ramona Nudischer ◽  
Kasper Renggli ◽  
Andreas Hierlemann ◽  
Adrian B. Roth ◽  
Cristina Bertinetti-Lapatki
Keyword(s):  
Immune Responses ◽  
Liver Toxicity ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Drug Induced ◽  
Tissue Model
Sign in / Sign up

Export Citation Format

Share Document