Feed pellets containing chitosan nanoparticles as plasmid DNA oral delivery system for fish: In vivo assessment in gilthead sea bream ( Sparus aurata ) juveniles

2018 ◽  
Vol 80 ◽  
pp. 458-466 ◽  
Author(s):  
M.I. Sáez ◽  
A.J. Vizcaíno ◽  
F.J. Alarcón ◽  
T.F. Martínez
Keyword(s):  
Delivery System ◽  
Plasmid Dna ◽  
Oral Delivery ◽  
Sparus Aurata ◽  
Chitosan Nanoparticles ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Oral Delivery System ◽  
In Vivo Assessment

scholarly journals Experimental Horizontal Transmission of Enterospora nucleophila (Microsporea: Enterocytozoonidae) in Gilthead Sea Bream (Sparus aurata)

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 362
Author(s):  
Amparo Picard-Sánchez ◽  
M. Carla Piazzon ◽  
Itziar Estensoro ◽  
Raquel Del Pozo ◽  
Nahla Hossameldin Ahmed ◽  
...  
Keyword(s):  
Sparus Aurata ◽  
Horizontal Transmission ◽  
Infected Fish ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Target Tissue ◽  
Farmed Fish ◽  
Infection Level ◽  
Available Information

Enterospora nucleophila is a microsporidian enteroparasite that infects mainly the intestine of gilthead sea bream (Sparus aurata), leading to an emaciative syndrome. Thus far, the only available information about this infection comes from natural outbreaks in farmed fish. The aim of the present study was to determine whether E. nucleophila could be transmitted horizontally using naturally infected fish as donors, and to establish an experimental in vivo procedure to study this host–parasite model without depending on natural infections. Naïve fish were exposed to the infection by cohabitation, effluent, or intubated either orally or anally with intestinal scrapings of donor fish in four different trials. We succeeded in detecting parasite in naïve fish in all the challenges, but the infection level and the disease signs were always milder than in donor fish. The parasite was found in peripheral blood of naïve fish at 4 weeks post-challenge (wpc) in oral and effluent routes, and up to 12 wpc in the anal transmission trial. Molecular diagnosis detected E. nucleophila in other organs besides intestine, such as gills, liver, stomach or heart, although the intensity was not as high as in the target tissue. The infection tended to disappear through time in all the challenge routes assayed, except in the anal infection route.

In vivo evaluation of an oral delivery system for P-gp substrates based on thiolated chitosan

Biomaterials ◽  
2006 ◽  
Vol 27 (23) ◽  
pp. 4250-4255 ◽  
Author(s):  
Florian Föger ◽  
Thierry Schmitz ◽  
Andreas Bernkop-Schnürch
Keyword(s):  
Delivery System ◽  
Oral Delivery ◽  
In Vivo Evaluation ◽  
Thiolated Chitosan ◽  
Oral Delivery System

In vivo and in vitro treatments against Sparicotyle chrysophrii (Monogenea: Microcotylidae) parasitizing the gills of gilthead sea bream (Sparus aurata L.)

Aquaculture ◽  
2006 ◽  
Vol 261 (3) ◽  
pp. 856-864 ◽  
Author(s):  
Ariadna Sitjà-Bobadilla ◽  
Magnolia Conde de Felipe ◽  
Pilar Alvarez-Pellitero
Keyword(s):  
Sparus Aurata ◽  
Gilthead Sea Bream ◽  
Sea Bream
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