Ex vivo approach supports both direct and indirect actions of melatonin on immunity in pike-perch Sander lucioperca

Author(s):  
Sébastien Baekelandt ◽  
Valérie Cornet ◽  
Syaghalirwa.N.M. Mandiki ◽  
Lambert Jérôme ◽  
Dubois Mickaël ◽  
...  
2021 ◽  
Vol 72 ◽  
pp. 105099
Author(s):  
Lorena Polloni ◽  
Fernanda Van Petten Vasconcelos Azevedo ◽  
Samuel Cota Teixeira ◽  
Eloá Moura ◽  
Tassia Rafaela Costa ◽  
...  

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1799
Author(s):  
Michaela Schafberg ◽  
Karin Loest ◽  
Andreas Müller-Belecke ◽  
Sascha Rohn

In the last decades, several plant-based materials were used for the substitution of fish meal and oil in aquaculture. The present study evaluated the fish quality and the sensory differences of rainbow trout (Oncorhynchus mykiss) and pike-perch (Sander lucioperca) from three different feeding groups, which were fed a commercially available industrial (standard) diet, a control diet, and a special microorganism-based feed mix. This feed mainly consisted of a mix made of Rhodotorula glutinis, Crypthecodinium cohnii, and Arthrospira sp. and had 50% less fish meal and fish oil compared to typical control diets. At the beginning, the pike-perch population was six months old, and the rainbow trout population was 15 months old. The feeding study duration was 16 weeks and every four weeks the growth performance and several morphometric parameters were recorded. Afterwards, sensory evaluation took place to identify possible trends. Sensory evaluation revealed that the rainbow trout groups did not show any significant differences to the standard and control fish fillets with regard to odor, texture, and taste. The effects on rainbow trout growth performances and carcass parameters were similar to the standard group. The feed mix was not optimal for pike-perch farming, which was also reflected by significantly adversely affected growth performance and carcass parameters. The sensorial evaluation showed an opposite trend: here, only small differences in the fillets from the feed mix and standard/control diet were observed.


Cells ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 132
Author(s):  
Johanna Simon ◽  
Gabor Kuhn ◽  
Michael Fichter ◽  
Stephan Gehring ◽  
Katharina Landfester ◽  
...  

Understanding the behavior of nanoparticles upon contact with a physiological environment is of urgent need in order to improve their properties for a successful therapeutic application. Most commonly, the interaction of nanoparticles with plasma proteins are studied under in vitro conditions. However, this has been shown to not reflect the complex situation after in vivo administration. Therefore, here we focused on the investigation of magnetic nanoparticles with blood proteins under in vivo conditions. Importantly, we observed a radically different proteome in vivo in comparison to the in vitro situation underlining the significance of in vivo protein corona studies. Next to this, we found that the in vivo corona profile does not significantly change over time. To mimic the in vivo situation, we established an approach, which we termed “ex vivo” as it uses whole blood freshly prepared from an animal. Overall, we present a comprehensive analysis focusing on the interaction between nanoparticles and blood proteins under in vivo conditions and how to mimic this situation with our ex vivo approach. This knowledge is needed to characterize the true biological identity of nanoparticles.


2010 ◽  
Vol 196 ◽  
pp. S232-S233
Author(s):  
P. Pinton ◽  
D. Tsybulskyy ◽  
B. Joly ◽  
N. Bourges-Abella ◽  
I.P. Oswald ◽  
...  

Aquaculture ◽  
2019 ◽  
Vol 500 ◽  
pp. 550-561 ◽  
Author(s):  
Ivar Lund ◽  
Covadonga Rodríguez ◽  
Maria S. Izquierdo ◽  
Najlae El Kertaoui ◽  
Patrick Kestemont ◽  
...  

Metabolites ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 264 ◽  
Author(s):  
Tsentalovich ◽  
Yanshole ◽  
Yanshole ◽  
Zelentsova ◽  
Melnikov ◽  
...  

This work represents the first comprehensive report on quantitative metabolomic composition of tissues of pike-perch (Sander lucioperca) and Siberian roach (Rutilus rutilus lacustris). The total of 68 most abundant metabolites are identified and quantified in the fish lenses and gills by the combination of LC-MS and NMR. It is shown that the concentrations of some compounds in the lens are much higher than that in the gills; that indicates the importance of these metabolites for the adaptation to the specific living conditions and maintaining the homeostasis of the fish lens. The lens metabolome undergoes significant seasonal changes due to the variations of dissolved oxygen level and fish feeding activity. The most season-affected metabolites are osmolytes and antioxidants, and the most affected metabolic pathway is the histidine pathway. In late autumn, the major lens osmolytes are N-acetyl-histidine and threonine phosphoethanolamine (Thr-PETA), while in winter the highest concentrations were observed for serine phosphoethanolamine (Ser-PETA) and myo-inositol. The presence of Thr-PETA and Ser-PETA in fish tissues and their role in cell osmotic protection are reported for the first time. The obtained concentrations can be used as baseline levels for studying the influence of environmental factors on fish health.


2019 ◽  
Vol 27 (4) ◽  
pp. 991-1007 ◽  
Author(s):  
Dóra Kánainé Sipos ◽  
Gyula Kovács ◽  
Eszter Buza ◽  
Katalin Csenki-Bakos ◽  
Ágnes Ősz ◽  
...  

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