Corneocyte lysis and fragmented DNA considerations for the cellular component of forensic touch DNA

SummaryTreatment of washed rabbit platelets with 1 u/ml of thrombin at 37° C resulted in a disappearance from platelets of a protein with 250,000 dalton molecular weight which was shown to be originated from plasma membrane. Parallel loss of adenyl cyclase was noted, and both reactions were complete within 30 sec. From the patterns of disc electrophoretograms, the importance of quick suppression of thrombin action in demonstrating the primary event was stressed.Thrombin induced an apparent activation of membrane bound phosphodiesterase. This reaction was also complete within 30 sec. The cellular component which contained the enzyme activity was distinct from plasma membrane. Soluble phosphodiesterase was not influenced by thrombin at all.These reactions required intact platelet cells to react with thrombin, and no reaction was detected when subcellular preparations were treated with thrombin.Possibility of collaboration of changes in externally located synthetic enzyme with those in internally located degrading enzyme in the early phase of thrombin action on platelets was suggested.

Download Full-text

ROLE OF THE STROMAL CELLULAR COMPONENT IN COMPENSATORY PROCESSES IN DIFFUSE LIVER DAMAGE

Токсикологический вестник ◽

10.36946/0869-7922-2018-3-32-37 ◽

2018 ◽

pp. 32-37 ◽

Cited By ~ 1

Author(s):

Z. A. Shafigullina ◽

S. Yu. Medvedeva ◽

I. G. Danilova

Keyword(s):

Toxic Hepatitis ◽

Intraperitoneal Administration ◽

Sharp Decrease ◽

Cellular Component ◽

Ki 67 ◽

Sinusoidal Cells ◽

Toxic Damage ◽

Stromal Component ◽

Regeneration Processes

The aim of the study was to assess the role of the cellular component of the stroma in liver regeneration after its toxic damage. The experimental model of toxic hepatitis caused by intraperitoneal administration of tetrachloromethane (CCl4) showed that regeneration processes in the liver on the 3rd day are manifested in an increase in binuclear hepatocytes, Ki-67 + cells and hepatocytes dividing by mitosis. The reaction of the stromal component is expressed in an increase in the number of CD45 +, mast and sinusoidal cells (SC). On the 7th day of the development of toxic hepatitis the hepatocyte alteration increases, that is accompanied by a sharp decrease in the mitotic index and the number of Ki-67 + cells. In the stromal component there is a decrease in the number of sinusoidal cells, CD45 + and a significant increase in mast cells with a high secretion granule content.

Download Full-text

Evaluation of a fluorescent dye to visualize touch DNA on various substrates

Journal of Forensic Sciences ◽

10.1111/1556-4029.14695 ◽

2021 ◽

Author(s):

Jessica Champion ◽

Piyamas Kanokwongnuwut ◽

Roland A. H. Oorschot ◽

Duncan Taylor ◽

Adrian Linacre

Keyword(s):

Fluorescent Dye ◽

Touch Dna

Download Full-text

Odontoblast TRPC5 channels signal cold pain in teeth

Science Advances ◽

10.1126/sciadv.abf5567 ◽

2021 ◽

Vol 7 (13) ◽

pp. eabf5567

Author(s):

Laura Bernal ◽

Pamela Sotelo-Hitschfeld ◽

Christine König ◽

Viktor Sinica ◽

Amanda Wyatt ◽

...

Keyword(s):

Relative Sensitivity ◽

Cellular Component ◽

Sensory Transduction ◽

Cold Sensation ◽

Cold Pain ◽

Sensing System ◽

Cold Sensitive ◽

Cellular Components ◽

Cold Sensor ◽

Cold Sensing

Teeth are composed of many tissues, covered by an inflexible and obdurate enamel. Unlike most other tissues, teeth become extremely cold sensitive when inflamed. The mechanisms of this cold sensation are not understood. Here, we clarify the molecular and cellular components of the dental cold sensing system and show that sensory transduction of cold stimuli in teeth requires odontoblasts. TRPC5 is a cold sensor in healthy teeth and, with TRPA1, is sufficient for cold sensing. The odontoblast appears as the direct site of TRPC5 cold transduction and provides a mechanism for prolonged cold sensing via TRPC5’s relative sensitivity to intracellular calcium and lack of desensitization. Our data provide concrete functional evidence that equipping odontoblasts with the cold-sensor TRPC5 expands traditional odontoblast functions and renders it a previously unknown integral cellular component of the dental cold sensing system.

Download Full-text

Accurate genotyping of fragmented DNA using a toehold assisted padlock probe

Biosensors and Bioelectronics ◽

10.1016/j.bios.2021.113079 ◽

2021 ◽

Vol 179 ◽

pp. 113079

Author(s):

Yanmin Gao ◽

Hongyan Qiao ◽

Victor Pan ◽

Zhaoguan Wang ◽

Jiaojiao Li ◽

...

Keyword(s):

Padlock Probe ◽

Fragmented Dna

Download Full-text

Back to Amido Black : Uncovering touch DNA in blood‐contaminated fingermarks

Journal of Forensic Sciences ◽

10.1111/1556-4029.14783 ◽

2021 ◽

Author(s):

Yinon Harush‐Brosh ◽

Yael Levy‐Herman ◽

Ravell Bengiat ◽

Carla Oz ◽

Michal Levin‐Elad ◽

...

Keyword(s):

Touch Dna ◽

Amido Black

Download Full-text

Correlation between the DNA fragmentation index (DFI) and sperm morphology of infertile patients

Journal of Assisted Reproduction and Genetics ◽

10.1007/s10815-021-02080-w ◽

2021 ◽

Author(s):

Alberto Ferrigno ◽

Giovanni Ruvolo ◽

Giuseppina Capra ◽

Nicola Serra ◽

Liana Bosco

Keyword(s):

Dna Fragmentation ◽

Sperm Morphology ◽

Seminal Fluid ◽

Image Analyzer ◽

Fragmented Dna ◽

Fragmentation Index ◽

Group A ◽

Group B ◽

Infertile Patients ◽

Dna Fragmentation Index

Abstract Purpose To evaluate the correlation between the DNA Fragmentation Index (DFI) and sperm morphology in patients undergoing ICSI, as a predictive parameter in reproductive outcomes. Methods A retrospective study was conducted on 125 infertile patients enrolled in a fertility clinic. Seminal characteristics were measured following the WHO guidelines (2010) for the examination of the seminal fluid. After collecting motile sperm population by pellet swim up, DFI was calculated and simultaneously associated with sperm morphology using in situ TUNEL assay and an image analyzer software in at least 250 spermatozoa for each patient. Results All subjects were divided into two groups according to a cutoff established, by choice, of the sperm DFI (15%): group A (< 15%) consisting of 65 patients and group B (≥ 15%) of 60 patients. Data were analyzed using non-parametric statistical methods. The results demonstrate that there is no statistical difference between the two groups in seminal characteristics. The collective data show a high significant correlation, suggesting that spermatozoa with abnormal morphology are the best candidates to contain DNA damage (p < 0.001). Also, when group A is compared with group B, an increased percentage of morphologically normal spermatozoa with fragmented DNA was observed in patients, with DFI values ≥ 15% (p < 0.001). Conclusion These results are aimed at providing an exact value of DFI in morphologically normal spermatozoa, which will be helpful to the embryologist in evaluating the risk of transferring, during the ICSI procedure, a spermatozoon whit normal morphology but fragmented DNA.

Download Full-text