Human-associated migration of Holarctic Saccharomyces uvarum strains to Patagonia

ABSTRACT: Advances in the fields of glycobiology and immunology have provided many insights into the role of carbohydrate-protein interactions in the immune system. Jacalin of Artocarpus integrifolia (JCA) and structural mannoprotein of Saccharomyces uvarum (MPS) are molecules with immunomodulatory properties. JCA is an IgA human lectin binding molecule that causes the mitogenic stimulation of immune cells, production of cytokines, chemotaxis, and activation of leukocytes. Studies on the immunomodulatory properties of JCA and MPS in mammals and fish suggest that they have an action on antibody production. The aim of this study was to investigate the possible action of JCA and MPS on the production of specific antibodies in laying hens. For this, laying hens were inoculated with an intra abdominal injection of sheep red blood cells (SRBC) with either JCA (0.075 µg, 0.75 µg, and 7.5 µg) or MPS (20 µg and 100 µg). Levels of anti-SRBC antibodies of the IgY, IgM, and IgA classes were evaluated by ELISA. Results showed that JCA and MPS have immunomodulatory effects on levels of anti-SRBC IgM, IgA, and IgY. An immunostimulatory effect of JCA was observed in primary immune response on anti-SRBC IgY, while an inhibitory effect of JCA and MPS was observed in secondary immune response on the production of IgM and IgA anti-SRBC. These results suggested that MPS and JCA have immunomodulatory effects on antibody production and could be used in future studies on humoral immune response in poultry.

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Isotopomeric 13C Labeling of Amino Acids Reveal Compartmentation in Saccharomyces uvarum

Biophysical Journal ◽

10.1016/j.bpj.2008.12.1537 ◽

2009 ◽

Vol 96 (3) ◽

pp. 308a

Author(s):

David W. Schryer ◽

Pearu Peterson ◽

Toomas Paalme ◽

Marko Vendelin

Keyword(s):

Amino Acids ◽

Saccharomyces Uvarum ◽

13C Labeling

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Mitochondria-encoded genes contribute to evolution of heat and cold tolerance in yeast

Science Advances ◽

10.1126/sciadv.aav1848 ◽

2019 ◽

Vol 5 (1) ◽

pp. eaav1848 ◽

Cited By ~ 24

Author(s):

Xueying C. Li ◽

David Peris ◽

Chris Todd Hittinger ◽

Elaine A. Sia ◽

Justin C. Fay

Keyword(s):

Saccharomyces Cerevisiae ◽

Cold Tolerance ◽

Yeast Species ◽

Saccharomyces Uvarum ◽

Phenotypic Differences ◽

Protein Divergence ◽

Thermal Growth ◽

Genome Wide ◽

A Genome ◽

Moderate Effect

Genetic analysis of phenotypic differences between species is typically limited to interfertile species. Here, we conducted a genome-wide noncomplementation screen to identify genes that contribute to a major difference in thermal growth profile between two reproductively isolated yeast species,Saccharomyces cerevisiaeandSaccharomyces uvarum. The screen identified only a single nuclear-encoded gene with a moderate effect on heat tolerance, but, in contrast, revealed a large effect of mitochondrial DNA (mitotype) on both heat and cold tolerance. Recombinant mitotypes indicate that multiple genes contribute to thermal divergence, and we show that protein divergence inCOX1affects both heat and cold tolerance. Our results point to the yeast mitochondrial genome as an evolutionary hotspot for thermal divergence.

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Inheritance of winemaking stress factors tolerance in Saccharomyces uvarum/S. eubayanus × S. cerevisiae artificial hybrids

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2019.108500 ◽

2020 ◽

Vol 320 ◽

pp. 108500 ◽

Cited By ~ 2

Author(s):

Andrea Cecilia Origone ◽

Melisa González Flores ◽

María Eugenia Rodríguez ◽

Amparo Querol ◽

Christian Ariel Lopes

Keyword(s):

Stress Factors ◽

Saccharomyces Uvarum

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Glycolysis and respiration in yeasts. The Pasteur effect studied by mass spectrometry

Biochemical Journal ◽

10.1042/bj2120749 ◽

1983 ◽

Vol 212 (3) ◽

pp. 749-754 ◽

Cited By ~ 24

Author(s):

D Lloyd ◽

B Kristensen ◽

H Degn

Keyword(s):

Mass Spectrometry ◽

Schizosaccharomyces Pombe ◽

Candida Utilis ◽

Control Mechanism ◽

Saccharomyces Uvarum ◽

Pasteur Effect ◽

Continuous Measurements ◽

Inhibition Of Glycolysis ◽

Damped Oscillation

Simultaneous and continuous measurements of changes in CO2 and O2 concentrations in glucose-metabolizing yeast suspensions by mass spectrometry enabled a study of the Pasteur effect (aerobic inhibition of glycolysis) in Saccharomyces uvarum and Schizosaccharomyces pombe. A different control mechanism operates in Candida utilis to give a damped oscillation after the anaerobic-aerobic transition. The apparent Km values for respiration of the three yeasts were in the range 1.3-1.8 microM-O2. The apparent Km values for O2 of the Pasteur effect were 5 and 13 microM for catabolite-repressed and derepressed S. uvarum respectively and 7 microM for Sch. pombe. These results are discussed with respect to currently accepted mechanisms for the control of glycolysis.

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