Characterization of metabolic network of oxalic acid biosynthesis through RNA seq data analysis of developing spikes of finger millet ( Eleusine coracana ): Deciphering the role of key genes involved in oxalate formation in relation to grain calcium accumulation

Abstract Insect Hox3/zen genes represent an evolutionary hotspot for changes in function and copy number. Single orthologues are required either for early specification or late morphogenesis of the extraembryonic tissues, which protect the embryo. The tandemly duplicated zen paralogues of the beetle Tribolium castaneum present a unique opportunity to investigate both functions in a single species. We dissect the paralogues’ expression dynamics (transcript and protein) and transcriptional targets (RNA-seq after RNAi) throughout embryogenesis. We identify an unexpected role of Tc-Zen2 in repression of Tc-zen1, generating a negative feedback loop that promotes developmental progression. Tc-Zen2 regulation is dynamic, including within co-expressed multigene loci. We also show that extraembryonic development is the major event within the transcriptional landscape of late embryogenesis and provide a global molecular characterization of the extraembryonic serosal tissue. Altogether, we propose that paralogue mutual regulation arose through multiple instances of zen subfunctionalization, leading to their complementary extant roles.

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Cloning and characterization of auxin efflux carrier genes EcPIN1a and EcPIN1b from finger millet Eleusine coracana L.

3 Biotech ◽

10.1007/s13205-017-0689-6 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 3

Author(s):

Tapan Kumar Mohanta ◽

Hanhong Bae

Keyword(s):

Finger Millet ◽

Eleusine Coracana ◽

Auxin Efflux Carrier

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Unexpected mutual regulation underlies paralogue functional diversification and promotes maturation of a protective epithelial tissue

10.1101/427245 ◽

2018 ◽

Cited By ~ 1

Author(s):

Daniela Gurska ◽

Iris M. Vargas Jentzsch ◽

Kristen A. Panfilio

Keyword(s):

Feedback Loop ◽

Single Species ◽

Epithelial Tissue ◽

Rna Seq ◽

Developmental Progression ◽

Late Embryogenesis ◽

Mutual Regulation ◽

Transcriptional Landscape

ABSTRACTInsect Hox3/zen genes represent an evolutionary hotspot for changes in function and copy number. Single orthologues are required either for early specification or late morphogenesis of the extraembryonic tissues, which protect the embryo. The tandemly duplicated zen paralogues of the beetle Tribolium castaneum present a unique opportunity to investigate both functions in a single species. We dissect the paralogues’ expression dynamics (transcript and protein) and transcriptional targets (RNA-seq after RNAi) throughout embryogenesis. We identify an unexpected role of Tc-Zen2 in repression of Tc-zen1, generating a negative feedback loop that promotes developmental progression. Tc-Zen2 regulation is dynamic, including within co-expressed multigene loci. We also show that extraembryonic development is the major event within the transcriptional landscape of late embryogenesis and provide a global molecular characterization of the extraembryonic serosal tissue. Altogether, we propose that paralogue mutual regulation arose progressively and drove multiple instances of zen subfunctionalization, leading to complementary extant roles.

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