Optimising factors affecting solid phase extraction performances of molecular imprinted polymer as recent sample preparation technique

Background: Profiling the whole metabolome with a single injection is not an easy process because the chemical and physical properties of metabolites are totally different within each other and the analytical methodologies and datamining procedures need lots of effort to make such an approach for real. This reality leads researchers to select an already applied methodology for metabolite profiling and analyze the samples through identical techniques. Objective: In this study, it was focused whether the sample preparation techniques on human blood samples prior to QTOF LC/MS analysis affect the number of detectable peaks and to analyze the matched metabolites of these peaks. The results were compared within each other. Method: Precipitation of proteins with methanol, ultrafiltration (Amicon® Ultra 3 kDa 0.5 mL Centrifugal Filters), liquidphase extraction (EXtrelut® NT 3 cartridges) and solid-phase extraction (Supelco HybridSPE®-Phospholipid Cartridge) were used for sample preparation on commercial pooled plasmas samples. C18 column (Agilent Zorbax 1.8 μM, 50 x 2.1 mm) was used as the chromatography column. Q-TOF LC/MS analysis was performed on positive ionization mode. XCMS and MetaboAnalyst 4.0 - MS Peaks to Pathways utility were used to evaluate the raw data. Results: Although the number of detected peaks through precipitation with methanol was the highest one (624 peaks), the detected peaks observed through ultrafiltration sample preparation technique matched with the highest number of metabolite peaks (151 metabolites). The number of the matched peaks with metabolites on liquid phase extraction (81 metabolites) was higher than the ones for solid phase extraction (29 metabolites). Conclusion: The results in this study may provide a novel perspective to analytical chemists working with clinicians to select their sample preparation technique prior to Q-TOF LC/MS based untargeted metabolomic approaches.

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Solid-phase extraction with supercritical fluid elution as a sample preparation technique for the ultratrace analysis of flavone in blood plasma

Journal of Chromatography B Biomedical Sciences and Applications ◽

10.1016/0378-4347(92)80598-k ◽

1992 ◽

Vol 577 (1) ◽

pp. 61-67 ◽

Cited By ~ 12

Author(s):

Hanjiu Liu ◽

Kenneth R. Wehmeyer

Keyword(s):

Sample Preparation ◽

Solid Phase Extraction ◽

Blood Plasma ◽

Supercritical Fluid ◽

Solid Phase ◽

Phase Extraction ◽

Preparation Technique ◽

Ultratrace Analysis ◽

Sample Preparation Technique ◽

In Blood Plasma

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Supercritical fluid extraction combined with solid phase extraction as sample preparation technique for the analysis of β-blockers in serum and urine

Fresenius Journal of Analytical Chemistry ◽

10.1007/s002160050769 ◽

1998 ◽

Vol 360 (5) ◽

pp. 618-621 ◽

Cited By ~ 8

Author(s):

G. Meissner ◽

Kari Hartonen ◽

M.-L. Riekkola

Keyword(s):

Sample Preparation ◽

Solid Phase Extraction ◽

Supercritical Fluid Extraction ◽

Supercritical Fluid ◽

Solid Phase ◽

Phase Extraction ◽

Preparation Technique ◽

Sample Preparation Technique ◽

Β Blockers ◽

Serum And Urine

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Miniaturized solid-phase extraction as a sample preparation technique for the determination of phthalates in water

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-002-1270-6 ◽

2002 ◽

Vol 373 (1-2) ◽

pp. 81-86 ◽

Cited By ~ 66

Author(s):

Yoshihiro Saito ◽

Yuji Nakao ◽

Motohiro Imaizumi ◽

Yoriko Morishima ◽

Yoshiaki Kiso ◽

...

Keyword(s):

Sample Preparation ◽

Solid Phase Extraction ◽

Solid Phase ◽

Phase Extraction ◽

Preparation Technique ◽

Sample Preparation Technique

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Combined supercritical fluid extraction/solid-phase extraction with octadecylsilane cartridges as a sample preparation technique for the ultratrace analysis of a drug metabolite in plasma

Analytical Chemistry ◽

10.1021/ac00031a018 ◽

1992 ◽

Vol 64 (7) ◽

pp. 802-806 ◽

Cited By ~ 21

Author(s):

Hanjiu. Liu ◽

Linda M. Cooper ◽

Douglas E. Raynie ◽

J. David. Pinkston ◽

Kenneth R. Wehmeyer

Keyword(s):

Sample Preparation ◽

Solid Phase Extraction ◽

Supercritical Fluid Extraction ◽

Supercritical Fluid ◽

Solid Phase ◽

Phase Extraction ◽

Preparation Technique ◽

Fluid Extraction ◽

Ultratrace Analysis ◽

Sample Preparation Technique

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DEVELOPMENT OF MOLECULAR IMPRINTED POLYMER SOLID PHASE EXTRACTION (MISPE) FOR SEPARATION NITROFURANTOIN RESIDUE IN CHICKEN EGGS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i6.17109 ◽

2017 ◽

Vol 10 (6) ◽

pp. 108

Author(s):

Sophi Damayanti ◽

Untung Gunawan ◽

Slamet Ibrahim

Keyword(s):

Solid Phase Extraction ◽

Analytical Methods ◽

Density Functional ◽

Solid Phase ◽

Basis Set ◽

Phase Extraction ◽

Molecular Imprinted Polymer ◽

Imprinted Polymer ◽

Dft Methods ◽

The Matrix

Background: The use of nitrofurantoin and other nitrofuran antibiotics in food which produced from animals is prohibited by European Union because of potentially carcinogenic and mutagenic. Various methods for analysis of residues of nitrofurantoin has been developed, but because of the interference of the matrix, it is necessary to separate the matrix therefore, the matrix effect will not interfere the analysis. Nowadays, molecular imprinted polymer (MIP) is a well-developed tool in the analytical field, mainly for separating substances in relatively complex matrices.Objective: The purpose of this study is to obtain MISPE that is selective for the separation of nitrofurantoin residues in chicken eggs.Methods: Analytical methods development of nitrofurantoin were optimization of HPLC system and validation of analytical methods performed to obtain the suitable system for nitrofurantoin detection. In silico study used for MIP design by observing the difference Gibbs free energy using Gaussview 5.08 software with Density Functional Theory (DFT) methods using 6-311G as basis set. MIP synthesis was done using bulk method use nitrofurantoin as template, acrylamide as functional monomer, ethyleneglycoldimethacrylate (EGDMA) as crosslinker, and azobisisobutyronitrile (AIBN) as an initiator reaction inside dimethylformamide (DMF) as solvent. Non imprinted polymer (NIP) was synthesized as comparison. MIP and NIP which has been synthesized was inserted into SPE cartridge and characterized using Infrared spectroscopy and HPLC.Result: MISPE that has been synthesized was characterized and compared to non-imprinted polymer solid phase extraction (NISPE) and marketed Solid Phase Extraction (SPE) C18. Sensitivity of MIP, NIP, and SPE C-18 to nitrofurantoin was 84.54 %, 37.73 %, and 33.95 % respectively, based on recovery of nitrofurantoin.Conclusion: Based on the result it was obtained MISPE has high selectivity toward nitrofurantoin compared to NISPE and either marketed SPE.

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