Identification of differentially expressed genes in an industrial bioleaching heap processing low-grade copper sulphide ore elucidated by RNA arbitrarily primed polymerase chain reaction

The first adult-repopulating hematopoietic stem cells (HSCs) emerge in the mouse aorta-gonad-mesonephros (AGM) region at embryonic day 10.5 prior to their appearance in the yolk sac and fetal liver. Although several genes are implicated in the regulation of HSCs, there are gaps in our understanding of the processes taking place in the AGM at the time of HSC emergence. To identify genes involved in AGM HSC emergence, we performed differential display reverse transcriptase–polymerase chain reaction (DD RT-PCR). Differentially expressed genes included β-catenin and homologs of human TM9SF2 and TAB2. We characterized the expression pattern of Wnt/β-catenin signaling,mTM9SF2, and mTAB2 in the embryo and adult. Interestingly, the expression of mouse TAB2 (mTAB2) in the E11 dorsal aorta endothelium suggests a role for mTAB2 in HSC emergence and/or regulation. The identification of differentially expressed genes in the AGM region should yield further insights into the development of this tissue and into the emergence and regulation of HSCs.

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Identification of differentially expressed genes in rheumatoid arthritis by a combination of complementary DNA array and RNA arbitrarily primed-polymerase chain reaction

Arthritis & Rheumatism ◽

10.1002/1529-0131(200201)46:1<52::aid-art10048>3.0.co;2-1 ◽

2002 ◽

Vol 46 (1) ◽

pp. 52-63 ◽

Cited By ~ 39

Author(s):

E. Neumann ◽

F. Kullmann ◽

M. Judex ◽

H. P. J�sten ◽

D. Wessinghage ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Polymerase Chain Reaction ◽

Differentially Expressed Genes ◽

Differentially Expressed ◽

Dna Array ◽

Chain Reaction ◽

Complementary Dna ◽

Polymerase Chain

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All advanced stage non-Hodgkin's lymphomas with a polymerase chain reaction amplifiable breakpoint of bcl-2 have residual cells containing the bcl-2 rearrangement at evaluation and after treatment

Blood ◽

10.1182/blood.v78.12.3275.3275 ◽

1991 ◽

Vol 78 (12) ◽

pp. 3275-3280 ◽

Cited By ~ 244

Author(s):

JG Gribben ◽

As Freedman ◽

SD Woo ◽

K Blake ◽

RS Shu ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Salvage Chemotherapy ◽

Pcr Analysis ◽

Low Grade ◽

Advanced Stage ◽

Chain Reaction ◽

Additional Information ◽

Minimal Disease ◽

Polymerase Chain ◽

Hodgkin's Lymphomas

Abstract Polymerase chain reaction (PCR) of bcl-2 provides an extremely sensitive method to detect minimal disease in approximately 50% of patients with non-Hodgkin's lymphomas (NHL). In an attempt to determine the clinical usefulness of this technique, we examined the bone marrow (BM) of 152 patients with advanced-stage NHL at the time of evaluation and after induction or salvage chemotherapy before autologous BM transplantation. The BM proved to be an accessible and reproducible tissue source to determine PCR positivity because all of the 102 patients examined had the same PCR-amplifiable breakpoint in their BM and lymph node. At the time of evaluation, PCR analysis in advanced- stage NHL patients added little additional information to morphologic analysis because each technique identified BM infiltration in approximately 70% of patients. PCR was significantly more useful in determining BM infiltration after induction or salvage therapy. At that time, approximately 50% of patients had morphologically normal BM, whereas PCR analysis remained positive in 100% of those with an amplifiable breakpoint. These observations were confirmed in a clinical trial attempting to induce remission in previously untreated low-grade advanced-stage NHL patients. In this series, PCR was positive in all patients after treatment although the BM was histologically uninvolved in 50% of cases, showing that conventional therapy did not eradicate bcl-2-positive cells.

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Culture-Negative Endocarditis Diagnosed Using 16S DNA Polymerase Chain Reaction

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2012/312607 ◽

2012 ◽

Vol 23 (4) ◽

pp. 216-218 ◽

Cited By ~ 4

Author(s):

Stephen Duffett ◽

Bayan Missaghi ◽

Peter Daley

Keyword(s):

Polymerase Chain Reaction ◽

Dna Polymerase ◽

Tissue Sample ◽

Low Grade ◽

Chain Reaction ◽

Severe Aortic Regurgitation ◽

Valve Tissue ◽

Polymerase Chain ◽

Dna Pcr ◽

Culture Negative

16S DNA polymerase chain reaction (PCR) is a molecular amplification technique that can be used to identify bacterial pathogens in culture-negative endocarditis. Bacterial DNA can be isolated from surgically excised valve tissue or from blood collected in EDTA vials. Use of this technique is particularly helpful in identifying the bacterial pathogen in cases of culture-negative endocarditis. A case involving a 48-year-old man who presented with severe aortic regurgitation and a four-month prodrome of low-grade fever is reported. Blood and valve tissue cultures following valve replacement were negative. A valve tissue sample was sent for investigation with 16S DNA PCR, which successfully identifiedStreptococcus salivariusand was interpreted as the true diagnosis. A review of the literature suggests that 16S DNA PCR from valve tissue is a more sensitive diagnostic test than culture. It is also extremely specific, based on a sequence match of at least 500 base pairs.

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