Development of a Low-cost Polymerase Chain Reaction-based Method for Studying Differentially Expressed Genes in Developing Rice Leaves

2009 ◽  
Vol 51 (6) ◽  
pp. 614-621 ◽  
Author(s):  
Yin-Wan Wendy Fung ◽  
Hoi Yee Chow ◽  
Tik Wan Law ◽  
Biao Dong ◽  
Hoi Shan Kwan
Keyword(s):  
Polymerase Chain Reaction ◽  
Differentially Expressed Genes ◽  
Low Cost ◽  
Differentially Expressed ◽  
Chain Reaction ◽  
Rice Leaves ◽  
Polymerase Chain

scholarly journals Identification of 2 novel genes developmentally regulated in the mouse aorta-gonad-mesonephros region

Blood ◽  
2003 ◽  
Vol 101 (6) ◽  
pp. 2246-2249 ◽  
Author(s):  
Claudia Orelio ◽  
Elaine Dzierzak
Keyword(s):  
Polymerase Chain Reaction ◽  
Differentially Expressed Genes ◽  
Fetal Liver ◽  
Differentially Expressed ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Developmentally Regulated ◽  
Hematopoietic Stem ◽  
Polymerase Chain ◽  
Mouse Aorta

The first adult-repopulating hematopoietic stem cells (HSCs) emerge in the mouse aorta-gonad-mesonephros (AGM) region at embryonic day 10.5 prior to their appearance in the yolk sac and fetal liver. Although several genes are implicated in the regulation of HSCs, there are gaps in our understanding of the processes taking place in the AGM at the time of HSC emergence. To identify genes involved in AGM HSC emergence, we performed differential display reverse transcriptase–polymerase chain reaction (DD RT-PCR). Differentially expressed genes included β-catenin and homologs of human TM9SF2 and TAB2. We characterized the expression pattern of Wnt/β-catenin signaling,mTM9SF2, and mTAB2 in the embryo and adult. Interestingly, the expression of mouse TAB2 (mTAB2) in the E11 dorsal aorta endothelium suggests a role for mTAB2 in HSC emergence and/or regulation. The identification of differentially expressed genes in the AGM region should yield further insights into the development of this tissue and into the emergence and regulation of HSCs.

scholarly journals Design and development of polymerase chain reaction thermal cycler using proportional-integral temperature controller

2018 ◽  
Vol 14 (2) ◽  
pp. 213-218
Author(s):  
Chong Kim Soon ◽  
Nawoor Anusha Devi ◽  
Kok Beng Gan ◽  
Sue-Mian Then
Keyword(s):  
Polymerase Chain Reaction ◽  
Low Cost ◽  
Maximum Temperature ◽  
Gradient System ◽  
Chain Reaction ◽  
Temperature Controller ◽  
Proportional Integral ◽  
Integral Temperature ◽  
Polymerase Chain ◽  
Thermal Cycler

A thermal cycler is used to amplify segments of DNA using the polymerase chain reaction (PCR). It is an instrument that requires precise temperature control and rapid temperature changes for certain experimental protocols. However, the commercial thermal cyclers are still bulky, expensive and limited for laboratory use only.  As such it is difficult for on-site molecular screening and diagnostics. In this work, a portable and low cost thermal cycler was designed and developed. The thermal cycler block was designed to fit six microcentrifuge tubes. A Proportional-Integral temperature controller was used to control the thermal cycler block temperature. The results showed that the maximum temperature ramp rate of the developed thermal cycler was 5.5 °C/s. The proportional gain (Kp) and integral gain (Ki) of the PI controller were 15 A/V and 1.8 A/Vs respectively. Finally, the developed thermal cycler successfully amplified six DNA samples at the expected molecular weight of 150 base pair. It has been validated using the Eppendorf Mastercycler nexus gradient system and gel electrophoresis analysis

Immuno-capture PCR method for detecting Acidovorax avenae subsp. citrulli from watermelon

2007 ◽  
Vol 4 (2) ◽  
pp. 173-179 ◽  
Author(s):  
Wang Xiao ◽  
Zhang Le ◽  
Xu Fu-Shou ◽  
Zhao Li-Han ◽  
Xie Guan-Lin
Keyword(s):  
Polymerase Chain Reaction ◽  
Low Cost ◽  
Chain Reaction ◽  
Direct Pcr ◽  
Causal Organism ◽  
Bacterial Fruit Blotch ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Honeydew Melon ◽  
Melon Seeds

AbstractAn immuno-capture polymerase chain reaction (IC-PCR) method for detection of Acidovorax avenae subsp. citrulli (AAC), the causal organism of bacterial fruit blotch (BFB) of watermelon, was developed by combining the immunosorbent enrichment (ISE) method with classical PCR and comparing with the direct PCR and growth check methods. The results showed that all A. avenae subsp. citrulli strains tested have produced 360 bp specific fragments using IC-PCR and direct PCR methods, while other strains from 10 different genera showed negative PCR results. The minimum detection concentration was about 50–100 cfu/ml and 104 cfu/ml, respectively. The IC-PCR sensitivity was 100 times higher than that of direct PCR. The examination of seven batches of different melon seeds from the markets by IC-PCR showed that one cantaloupe, two honeydew melon and two watermelon seed varieties carried the pathogen, indicating that the IC-PCR is an accurate, sensitive, rapid and low-cost technique.

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