Response to Letter to the Editor: Growth kinetics of Escherichia coli O157:H7 in mechanically-tenderized beef. International Journal of Food Microbiology, 140: 40–48

2011 ◽  
Vol 147 (1) ◽  
pp. 81-82 ◽  
Author(s):  
Lihan Huang
Keyword(s):  
Escherichia Coli ◽  
Growth Kinetics ◽  
Food Microbiology ◽  
Escherichia Coli O157 ◽  
Letter To The Editor ◽  
Kinetics Of

Response surface models for the growth kinetics of Escherichia coli O157:H7

1993 ◽  
Vol 10 (4) ◽  
pp. 303-315 ◽  
Author(s):  
R.L. Buchanan ◽  
L.K. Bagi ◽  
R.V. Goins ◽  
J.G. Phillips
Keyword(s):  
Escherichia Coli ◽  
Response Surface ◽  
Growth Kinetics ◽  
Escherichia Coli O157 ◽  
Response Surface Models ◽  
Surface Models ◽  
Kinetics Of

PCR for the Specific Detection of an Escherichia coli O157:H7 Laboratory Control Strain

2015 ◽  
Vol 78 (9) ◽  
pp. 1738-1744 ◽  
Author(s):  
MICHAEL KNOWLES ◽  
DOMINIC LAMBERT ◽  
GEORGE HUSZCZYNSKI ◽  
MARTINE GAUTHIER ◽  
BURTON W. BLAIS
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Control Measure ◽  
Positive Control ◽  
Food Microbiology ◽  
Escherichia Coli O157 ◽  
Control Strain ◽  
E Coli ◽  
Signature Sequences ◽  
Materials Used

Control strains of bacterial pathogens such as Escherichia coli O157:H7 are commonly processed in parallel with test samples in food microbiology laboratories as a quality control measure to assure the satisfactory performance of materials used in the analytical procedure. Before positive findings can be reported for risk management purposes, analysts must have a means of verifying that pathogenic bacteria (e.g., E. coli O157:H7) recovered from test samples are not due to inadvertent contamination with the control strain routinely handled in the laboratory environment. Here, we report on the application of an in-house bioinformatic pipeline for the identification of unique genomic signature sequences in the development of specific oligonucleotide primers enabling the identification of a common positive control strain, E. coli O157:H7 (ATCC 35150), using a simple PCR procedure.

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