Prevalence and characterization of Salmonella species isolated from pigs, ducks and chickens in Sichuan Province, China

Abstract Background Morganella morganii (M. morganii) is a member of the genera Morganella in the Enterobacteriaceae family.Methods To better understand the prevalence and characterization of M. morganii strains in cattle, 191 nasal swabs samples from beef cattle in Guang'an, Yibin, and Ziyang, Sichuan Province, China were collected. The presence of M. morganii in swabs was determined by PCR. Then positive swabs were processed by using bacterial isolation and identification. The M. morganii isolates were assayed for antimicrobial susceptibility and pathogenicity using a mouse model.Results The prevalence of Morgenella morganii in three cities was 10.5% (20/191). Three M. morganii strains (16GA7, 17GA61.2, and 17YB9) were isolated and identified from positive swabs samples. Phylogenetic analysis demonstrated that the 16S rRNA gene of M. morganii isolates could be clustered with known Genbank M. morganii strains. All three M. morganii strains were sensitive to penicillin-type and quinolone antibiotics, and were resistant to some cephalosporins, carbapenems, macrolide antibiotics and polymyxin B. Lesions in mice inoculated with M. morganii included pulmonary hemorrhage, thickened alveolar walls, and pulmonary inflammatory cell infiltration, although lesions varied by strain.Conclusion To the best of our knowledge, the present study is the first to report the prevalence of M. morganii in beef cattle. Through research on the M. morganii in beef cattle, it is found that mice were susceptible to M. morganii isolates, which can cause mice dead. However, the pathogenicity of M. morganii isolates on cattle still needs further study.

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Monitoring and biochemical characterization of beta-cypermethrin resistance in Spodoptera exigua (Lepidoptera: Noctuidae) in Sichuan Province, China

Pesticide Biochemistry and Physiology ◽

10.1016/j.pestbp.2018.02.008 ◽

2018 ◽

Vol 146 ◽

pp. 71-79 ◽

Cited By ~ 16

Author(s):

Xuegui Wang ◽

Xing Xiang ◽

Huiling Yu ◽

Shuhua Liu ◽

Yong Yin ◽

...

Keyword(s):

Spodoptera Exigua ◽

Biochemical Characterization ◽

Sichuan Province ◽

Lepidoptera Noctuidae

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Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China

Iranian Journal of Parasitology ◽

10.18502/ijpa.v14i1.723 ◽

2019 ◽

Author(s):

XinLei WANG ◽

Ling DONG ◽

Li ZHANG ◽

Yan LV ◽

Qian LI ◽

...

Keyword(s):

Genetic Diversity ◽

Toxoplasma Gondii ◽

Nested Pcr ◽

Genetic Characterization ◽

Sichuan Province ◽

Southwestern China ◽

Wild Rodents ◽

New Genotype ◽

Pcr Rflp

Background: Wild rodents are the intermediate hosts of Toxoplasma gondii. The distribution of genetic diversity of T. gondii in wild rodents is of importance to understand the transmission of this parasite. This study aimed to genetically characterize T. gondii isolates from wild rodents in Sichuan province, southwestern China in 2013. Methods: Genomic DNA was extracted from 10 g wild rodents’ brain samples. Semi-nested PCR and multilocous PCR-RFLP technology were performed to examine genetic diversity of T. gondii isolates as described previously. Results: Overall, 181 brain tissues of different wild rodents, including Eothenomys miletus (n=88), Crocidura attenuate (n=9), Rattus rattus sladeni (n=46), Mus musculus Linnaeus (n=6) and R. niviventer (n=32) were tested for T. gondii DNA, respectively. Six of them were positive for the T. gondii B1 gene by semi-nested PCR amplification, 4 showed complete genotyping results for all 11 polymorphic loci (SAG1, SAG2, alt. SAG2, SAG3, BTUB, GRA6, L358, PK1, C22-8, C29-2 and Apico) by PCR-RFLP, determined to represent a potential new genotype (http://toxodb.org/toxo/). Conclusion: These results documented genetic characterization of T. gondii in wild rodents from Sichuan province, and enriched the genetic diversity of T. gondii in China.

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