Hydrogen production by Clostridium thermocellum 27405 from cellulosic biomass substrates

2006 ◽  
Vol 31 (11) ◽  
pp. 1496-1503 ◽  
Author(s):  
D LEVIN ◽  
R ISLAM ◽  
N CICEK ◽  
R SPARLING
Keyword(s):  
Hydrogen Production ◽  
Clostridium Thermocellum ◽  
Cellulosic Biomass

Anaerobic membrane gas extraction facilitates thermophilic hydrogen production fromClostridium thermocellum

2018 ◽  
Vol 4 (11) ◽  
pp. 1771-1782 ◽  
Author(s):  
Scott Singer ◽  
Lauren Magnusson ◽  
Dianxun Hou ◽  
Jonathan Lo ◽  
Pin-Ching Maness ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Clostridium Thermocellum ◽  
Dark Fermentation ◽  
Cellulosic Biomass ◽  
Gas Extraction

Clostridium thermocellumis among the most efficient bacteria to convert cellulosic biomass into H2during dark fermentation.

Investigation on hydrogen production from paper sludge without inoculation and its enhancement by Clostridium thermocellum

2018 ◽  
Vol 263 ◽  
pp. 120-127 ◽  
Author(s):  
Qian An ◽  
Ji-Lian Wang ◽  
Yu-Tao Wang ◽  
Zhang-Lin Lin ◽  
Ming-Jun Zhu
Keyword(s):  
Hydrogen Production ◽  
Clostridium Thermocellum ◽  
Paper Sludge

Ethanol from cellulosic biomass

1983 ◽  
Vol 300 (1100) ◽  
pp. 323-333 ◽  
Keyword(s):  
Clostridium Thermocellum ◽  
Liquid Fuel ◽  
Thermophilic Bacteria ◽  
Cellulosic Biomass ◽  
Anaerobic Microorganism ◽  
Direct Production ◽  
Effective Utilization ◽  
Mutant Strains ◽  
Total Utilization ◽  
Clostridium Thermosaccharolyticum

The major objective of this project is to achieve the direct microbiological conversion of cellulosic biomass to liquid fuel, ethanol. Within the scope of this objective, it is also the intent to maximize the conversion efficiency of ethanol production from biomass. This can be achieved through the effective utilization of both the cellulosic (six-carbon sugar) and hemicellulosic (five-carbon sugar) fractions in biomass. The degradation of cellulosic biomass is achieved through the use of a thermophilic and anaerobic bacterium, Clostridium thermocellum . This microorganism is unique in that it is able to hydrolyse both the cellulosic and hemicellulosic fractions of biomass but, unfortunately, it is not able to metabolize the pentoses. Therefore, to achieve total utilization of biomass, a second thermophilic and anaerobic microorganism, Clostridium thermosaccharolyticum , has been under study owing to its ability to convert pentoses to ethanol. Mutation, selection and adaption programmes have yielded ethanol tolerant strains of both organisms. A fermentation process using mutant strains of the anaerobic, thermophilic bacteria Clostridium thermocellum and Clostridium thermosaccharolyticum has been investigated for the direct production of ethanol from agricultural cellulosics. Through strain improvements for increased ethanol tolerance and catabolite selectivity, alcohol yields of 85% of the theoretical maximum have been obtained from solka floc with mixed culture. The method of isolation and the performance of these improved strains on both refined cellulosics and a realistic biomass, corn [maize] stover, is presented in detail.

scholarly journals Metabolome Analysis of Constituents in Membrane Vesicles for Clostridium thermocellum Growth Stimulation

2021 ◽  
Vol 9 (3) ◽  
pp. 593
Author(s):  
Shunsuke Ichikawa ◽  
Yoichiro Tsuge ◽  
Shuichi Karita
Keyword(s):  
Growth Rate ◽  
Clostridium Thermocellum ◽  
Consolidated Bioprocessing ◽  
Membrane Vesicle ◽  
Single Gene ◽  
Membrane Vesicles ◽  
Growth Stimulation ◽  
Cellulosic Biomass ◽  
Cellulolytic Bacterium ◽  
Metabolome Analysis

The cultivation of the cellulolytic bacterium, Clostridium thermocellum, can have cost-effective cellulosic biomass utilizations, such as consolidated bioprocessing, simultaneous biological enzyme production and saccharification. However, these processes require a longer cultivation term of approximately 1 week. We demonstrate that constituents of the C. thermocellum membrane vesicle fraction significantly promoted the growth rate of C. thermocellum. Similarly, cell-free Bacillus subtilis broth was able to increase C. thermocellum growth rate, while several B. subtilis single-gene deletion mutants, e.g., yxeJ, yxeH, ahpC, yxdK, iolF, decreased the growth stimulation ability. Metabolome analysis revealed signal compounds for cell–cell communication in the C. thermocellum membrane vesicle fraction (ethyl 2-decenoate, ethyl 4-decenoate, and 2-dodecenoic acid) and B. subtilis broth (nicotinamide, indole-3-carboxaldehyde, urocanic acid, nopaline, and 6-paradol). These findings suggest that the constituents in membrane vesicles from C. thermocellum and B. subtilis could promote C. thermocellum growth, leading to improved efficiency of cellulosic biomass utilization.

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