Phytosynthesized iron oxide nanoparticles and ferrous iron on fermentative hydrogen production using Enterobacter cloacae: Evaluation and comparison of the effects

2014 ◽  
Vol 39 (23) ◽  
pp. 11920-11929 ◽  
Author(s):  
Sundaresan Mohanraj ◽  
Shanmugam Kodhaiyolii ◽  
Mookan Rengasamy ◽  
Velan Pugalenthi
Keyword(s):  
Iron Oxide ◽  
Hydrogen Production ◽  
Iron Oxide Nanoparticles ◽  
Ferrous Iron ◽  
Enterobacter Cloacae ◽  
Oxide Nanoparticles ◽  
Fermentative Hydrogen Production ◽  
Fermentative Hydrogen

scholarly journals Supplementation of green-synthesized nickel oxide nanoparticles enhances biohydrogen production of Klebsilla sp. using lignocellulosic hydrolysate and elucidation of the regulatory mechanism

2021 ◽  
Author(s):  
Qin Zhang ◽  
Siyuan Xu ◽  
Yanbin Li ◽  
Pengfei Ding ◽  
Yonggui Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hydrogen Production ◽  
Nickel Oxide ◽  
Production System ◽  
Regulatory Mechanism ◽  
Oxide Nanoparticles ◽  
Nickel Oxide Nanoparticles ◽  
Fermentative Hydrogen Production ◽  
Fermentative Hydrogen ◽  
Nio Nps

Abstract Background In recent years, adding nanoparticles to fermentative hydrogen production system has become an effective way to increase the biohydrogen yield, however, the application of green synthesized nanoparticles to hydrogen production system is rarely studied, even to the interpretation of the regulatory mechanism, there are few reports on the regulation of hydrogen production pathway and related gene expression by addition of nanoparticles. Thus, we herein reported the green synthesis of nickel oxide nanoparticles (NiO-NPs) from Eichhornia crassipes (Ec) extract for the first time, and evaluated the regulatory effect of these NPs on fermentative hydrogen production. Results Characterization of the Ec-NiO-NPs revealed their spherical shape, small diameter (9.1 ± 2.6 nm) and high purity. The maximum cumulative hydrogen production and hydrogen yield Y(H2/S) reached 4842.19 ± 23.43 mL/L and 101.45 ± 3.32 mL/gsubstrate, respectively, in the presence of 20 mg/L Ec-NiO-NP, which were 47.29% and 37.78% higher than the control without NPs addition. Evaluation of glucose and xylose utilization efficiency as well as key node metabolites further established the potential of Ec-NiO-NP to improve the reducing sugar utilization and metabolic flux distribution in hydrogen synthesis pathway. Furthermore, addition of 20 mg/L Ec-NiO-NP resulted in enhanced hydrogenase activity with a maximum increase of 623% comparing to the control, and led to changes in the gene expression of both hydrogenase and formate-hydrogen lyase, which play important roles in promoting hydrogen production at different stages of fermentation. Conclusions The results prove that supplementation with green-synthesized Ec-NiO-NP effectively improves fermentative hydrogen production and regulates key node metabolites alteration and functional gene expression. This study provides a cheap and eco-friendly method to enhance fermentative hydrogen production and new insights to reveal the regulatory mechanism underlying NP mediated increases in biohydrogen synthesis.

scholarly journals Phytosynthesized iron nanoparticles: effects on fermentative hydrogen production by Enterobacter cloacae DH-89

2015 ◽  
Vol 38 (6) ◽  
pp. 1533-1538 ◽  
Author(s):  
DHRUBAJYOTI NATH ◽  
AJAY KUMAR MANHAR ◽  
KULDEEP GUPTA ◽  
DEVABRATA SAIKIA ◽  
SHYMAL KUMAR DAS ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Iron Nanoparticles ◽  
Enterobacter Cloacae ◽  
Fermentative Hydrogen Production ◽  
Fermentative Hydrogen

Effect of some environmental parameters on fermentative hydrogen production by Enterobacter cloacae DM11

2006 ◽  
Vol 52 (6) ◽  
pp. 525-532 ◽  
Author(s):  
Kaushik Nath ◽  
Anish Kumar ◽  
Debabrata Das
Keyword(s):  
Hydrogen Production ◽  
Glucose Concentration ◽  
Environmental Parameters ◽  
Enterobacter Cloacae ◽  
Batch Process ◽  
Lag Phase ◽  
Initial Glucose Concentration ◽  
Fermentative Hydrogen Production ◽  
Production Of Hydrogen ◽  
Fermentative Hydrogen

Fermentative hydrogen production was carried out by Enterobacter cloacae DM11, using glucose as the substrate. The effects of initial substrate concentration, initial medium pH, and temperature were investigated. Results showed that at an initial glucose concentration of 1.0% (m/v), the molar yield of hydrogen was 3.31 mol (mol glucose)–1. However, at higher initial glucose concentration, both the rate and cumulative volume of hydrogen production decreased. The pH of 6.5 ± 0.2 at a temperature of 37 °C was found most suitable with respect to maximum rate of production of hydrogen in batch fermentation. Activation enthalpies of fermentation and that of thermal deactivation of the present process were estimated following a modified Arrhenius equation. The values were 47.34 and 118.67 kJ mol–1 K–1, respectively. The effect of the addition of Fe2+ on hydrogen production was also studied. It revealed that the presence of iron (Fe2+) in the media up to a concentration of 20 mg L–1 had a marginal enhancing effect on total hydrogen production. A simple model developed from the modified Gompertz equation was applied to estimate the hydrogen production potential, production rate, and lag-phase time in a batch process, based on the cumulative hydrogen production curves, using the software program Curve Expert 1.3. Key words: hydrogen, Enterobacter cloacae DM11, fermentation, glucose, H2 yield.

TARGETING CYCLIN B1 WITH ANTISENSE OLIGONUCLETOTIDES- COATED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Hosam Zaghloul ◽  
Doaa A. Shahin ◽  
Ibrahim El- Dosoky ◽  
Mahmoud E. El-awady ◽  
Fardous F. El-Senduny ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cellular Uptake ◽  
Superparamagnetic Iron Oxide ◽  
Cyclin B1 ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Mcf7 Cells ◽  
M Phase ◽  
The Impact

Antisense oligonucleotides (ASO) represent an attractive trend as specific targeting molecules but sustain poor cellular uptake meanwhile superparamagnetic iron oxide nanoparticles (SPIONs) offer stability of ASO and improved cellular uptake. In the present work we aimed to functionalize SPIONs with ASO targeting the mRNA of Cyclin B1 which represents a potential cancer target and to explore its anticancer activity. For that purpose, four different SPIONs-ASO conjugates, S-M (1–4), were designated depending on the sequence of ASO and constructed by crosslinking carboxylated SPIONs to amino labeled ASO. The impact of S-M (1–4) on the level of Cyclin B1, cell cycle, ROS and viability of the cells were assessed by flowcytometry. The results showed that S-M3 and S-M4 reduced the level of Cyclin B1 by 35 and 36%, respectively. As a consequence to downregulation of Cyclin B1, MCF7 cells were shown to be arrested at G2/M phase (60.7%). S-M (1–4) led to the induction of ROS formation in comparison to the untreated control cells. Furthermore, S-M (1–4) resulted in an increase in dead cells compared to the untreated cells and SPIONs-treated cells. In conclusion, targeting Cyclin B1 with ASO-coated SPIONs may represent a specific biocompatible anticancer strategy.

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