H2 production by Escherichia coli batch cultures during utilization of acetate and mixture of glycerol and acetate

2015 ◽  
Vol 40 (36) ◽  
pp. 12187-12192 ◽  
Author(s):  
Karen Trchounian ◽  
Harutyun Sargsyan ◽  
Armen Trchounian
1988 ◽  
Vol 66 (2) ◽  
pp. 187-191 ◽  
Author(s):  
Norio Shimizu ◽  
Shinichi Fukuzono ◽  
Kiyoshi Fujimori ◽  
Nabuko Nishimura ◽  
Yoji Odawara

1994 ◽  
Vol 16 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Jérome Lefebvre ◽  
Guy-Alain Junter ◽  
Jean-Claude Vincent

2010 ◽  
Vol 45 (8) ◽  
pp. 1334-1341 ◽  
Author(s):  
Juan-Miguel Puertas ◽  
Jordi Ruiz ◽  
Mónica Rodríguez de la Vega ◽  
Julia Lorenzo ◽  
Glòria Caminal ◽  
...  

2020 ◽  
Vol 367 (11) ◽  
Author(s):  
Hripsime Petrosyan ◽  
Liana Vanyan ◽  
Satenik Mirzoyan ◽  
Armen Trchounian ◽  
Karen Trchounian

ABSTRACT After brewing roasted coffee, spent coffee grounds (SCGs) are generated being one of the daily wastes emerging in dominant countries with high rate and big quantity. Escherichia coli BW25113 wild-type strain, mutants with defects in hydrogen (H2)-producing/oxidizing four hydrogenases (Hyd) (ΔhyaB ΔhybC, ΔhycE, ΔhyfG) and septuple mutant (ΔhyaB ΔhybC ΔhycA ΔfdoG ΔldhA ΔfrdC ΔaceE) were investigated by measuring change of external pH, bacterial growth and H2 production during the utilization of SCG hydrolysate. In wild type, H2 was produced with rate of 1.28 mL H2 (g sugar)−1 h−1 yielding 30.7 mL H2 (g sugar)−1 or 2.75 L (kg SCG)−1 during 24 h. In septuple mutant, H2 production yield was 72 mL H2 (g sugar)−1 with rate of 3 mL H2 (g sugar)−1 h−1. H2 generation was absent in hycE single mutant showing the main role of Hyd-3 in H2 production. During utilization of SCG wild type, specific growth rate was 0.72 ± 0.01 h−1 with biomass yield of 0.3 g L−1. Genetic modifications and control of external parameters during growth could lead to prolonged and enhanced microbiological H2 production by organic wastes, which will aid more efficiently global sustainable energy needs resulting in diversification of mobile and fixed energy sources.


2010 ◽  
Vol 33 (7) ◽  
pp. 1112-1124 ◽  
Author(s):  
S. Tebbani ◽  
D. Dumur ◽  
G. Hafidi ◽  
A. Vande Wouwer

2008 ◽  
Vol 43 (7) ◽  
pp. 507-512 ◽  
Author(s):  
Jaume Pinsach ◽  
Carles de Mas ◽  
Josep López-Santín ◽  
Gerald Striedner ◽  
Karl Bayer

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