Enhanced aerobic H2 production by engineering an [FeFe] hydrogenase from Clostridium pasteurianum

2020 ◽  
Vol 45 (18) ◽  
pp. 10673-10679 ◽  
Author(s):  
Jamin Koo
Keyword(s):  
H2 Production ◽  
Clostridium Pasteurianum

scholarly journals Self-Synchronized Oscillatory Metabolism of Clostridium pasteurianum in Continuous Culture

Processes ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 137 ◽  
Author(s):  
Erin E Johnson ◽  
Lars Rehmann
Keyword(s):  
Redox Potential ◽  
Continuous Culture ◽  
H2 Production ◽  
Gas Production ◽  
Glycerol Metabolism ◽  
Clostridium Pasteurianum ◽  
Continuous Stirred Tank Reactor ◽  
Industrial Fermentation ◽  
Oxidative Pathway ◽  
Product Profile

By monitoring the real-time gas production (CO2 and H2) and redox potential at high sampling frequency in continuous culture of Clostridium pasteurianum on glycerol as sole carbohydrate, the self-synchronized oscillatory metabolism was revealed and studied. The oscillations in CO2 and H2 production were in sync with each other and with both redox potential and glycerol in the continuous stirred tank reactor (CSTR). There is strong evidence that the mechanism for this is in the regulation of the oxidative pathway of glycerol metabolism, including glycolysis, and points toward complex, concerted cycles of enzyme inhibition and activation by pathway intermediates and/or redox equivalents. The importance of understanding such an “oscillatory metabolism” is for developing a stable and highly productive industrial fermentation process for butanol production, as unstable oscillations are unproductive. It is shown that the oscillatory metabolism can be eradicated and reinstated and that the period of oscillations can be altered by modification of the operating parameters. Synchronized oscillatory metabolism impacted the product profile such that it lowered the selectivity for butanol and increased the selectivity for ethanol. This elucidates a possible cause for the variability in the product profile of C. pasteurianum that has been reported in many previous studies.

PERLAKUAN ETHYL METHANE SULFONATE (EMS) PADA ENTEROBACTER AEROGENES AY-2 DARI LIMBAH METAN FERMENTASI UNTUK PENINGKATAN PRODUKSI GAS HIDROGEN

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Mahyudin Abdul Rachman
Keyword(s):  
Metabolic Pathway ◽  
Acid Production ◽  
Double Mutant ◽  
Enterobacter Aerogenes ◽  
H2 Production ◽  
Hydrogen Gas ◽  
Survival Ratio ◽  
Ethyl Methane Sulfonate ◽  
Methane Sulfonate ◽  
Ethyl Methane

Enterobacter aerogenes AY-2 mutant is known for hydrogen gas producer which ws obtained from the sludge of methane fermentation and the yield is 1.5 fold higher than wildtype. Hydrogen gas production can be gain via NADH oxidation in anaerobic metabolic pathway by blocking organic acid production. Metabolic pathway can be changed by mutagenesis. Enterobacter aerogenes AY-2 mutated with ethyl methane sulfonate in logarithmic phase with consentration 10, 11, 12, 13, 14 and 15 μl/ml cell suspention during 120 minute. Mutation that result lowest survival ratio (0,01%) was 14 μl EMS/ml cell suspention is repeated with variation incubation time, 30, 60, 90 and 120 minute. 166 double mutant colony has been collected and choosen randomly. The choosen 43 colony was fermented in glycerol complex medium for determining ten double mutant with the highest H2 production. Double mutant AD-H43 is a highest H2 producer that increase 20% H2 production from AY-2 and has a decrease lactid acid production, 31% less from AY-2. Increasing H2 production in double mutant AD-H43 is caused by lactate dehydrogenase deffi cient.Keywords: Enterobacter aerogenes AY-2, ethyl methane sulfonate (EMS), H2 and methane sludge

scholarly journals The Hydrogenase System of Clostridium pasteurianum

1963 ◽  
Vol 238 (3) ◽  
pp. 1141-1144
Author(s):  
R.C. Valentine ◽  
L.E. Mortenson ◽  
J.E. Carnahan
Keyword(s):  
Clostridium Pasteurianum

Metabolically versatile Rhodobacter sphaeroides as a robust biocatalyst for H2 production from lignocellulose-derived mix substrates

Fuel ◽  
2021 ◽  
Vol 302 ◽  
pp. 121108
Author(s):  
Jun Hu ◽  
Wenwen Wei ◽  
Qing Li ◽  
Wen Cao ◽  
Anlong Zhang ◽  
...  
Keyword(s):  
Rhodobacter Sphaeroides ◽  
H2 Production
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