scholarly journals A Comparative Western Immunoblot Antibody Titration Test for Evaluation of Bartonella Therapy

2008 ◽  
Vol 12 ◽  
pp. e465-e466
Author(s):  
W.D. Hardy ◽  
E.E. Zuckerman
Keyword(s):  
Western Immunoblot

scholarly journals ELEVATION OF MATRIX METALLOPROTEINASES 3 AND 9 IN CEREBROSPINAL FLUID AND BLOOD IN PATIENTS WITH SEVERE TRAUMATIC BRAIN INJURY

Neurosurgery ◽  
2009 ◽  
Vol 65 (4) ◽  
pp. 702-708 ◽  
Author(s):  
Mark Grossetete ◽  
Jeremy Phelps ◽  
Leopold Arko ◽  
Howard Yonas ◽  
Gary A. Rosenberg
Keyword(s):  
Traumatic Brain Injury ◽  
Cerebrospinal Fluid ◽  
Brain Injury ◽  
Matrix Metalloproteinases ◽  
Blood Brain Barrier ◽  
Normal Pressure Hydrocephalus ◽  
Normal Pressure ◽  
Brain Barrier ◽  
Western Immunoblot ◽  
Blood Brain

Abstract OBJECTIVE Traumatic brain injury (TBI) causes an increase in matrix metalloproteinases (MMPs), which are associated with neuroinflammation, blood-brain barrier disruption, hemorrhage, and cell death. We hypothesized that patients with TBI have an increase in MMPs in ventricular cerebrospinal fluid (CSF) and plasma. METHODS Patients with TBI and a ventricular catheter were entered into the study. Samples of CSF and plasma were collected at the time of catheter placement and at 24 and 72 hours after admission. Seven TBI patients were entered into the study, with 6 having complete data for analysis. Only patients who had a known time of insult that fell within a 6-hour window from initial insult to ventriculostomy were accepted into the study. Control CSF came from ventricular fluid in patients undergoing shunt placement for normal pressure hydrocephalus. Both MMP-2 and MMP-9 were measured with gelatin zymography and MMP-3 with Western immunoblot. RESULTS We found a significant elevation in the levels of the latent form of MMP-9 (92-kD) in the CSF obtained at the time of arrival (P < 0.05). Elevated levels of MMP-2 were detected in plasma at 72 hours, but not in the CSF. Using albumin from both CSF and blood, we calculated the MMP-9 index, which was significantly increased in the CSF, indicating endogenous MMP production. Western immunoblot showed elevated levels of MMP-3 in CSF at all times measured, whereas MMP-3 was not detected in the CSF of normal pressure hydrocephalus. CONCLUSION We show that MMPs are increased in the CSF of TBI patients. Although the number of patients was small, the results were robust and clearly demonstrated increases in MMP-3 and MMP-9 in ventricular CSF in TBI patients compared with controls. Although these preliminary results will need to be replicated, we propose that MMPs may be important in blood-brain barrier opening and hemorrhage secondary to brain injury in patients.

Demonstration of Low Molecular Weight Polypeptides Associated with Small, Round-Structured Viruses by Western Immunoblot Analysis

1992 ◽  
Vol 36 (10) ◽  
pp. 1105-1112 ◽  
Author(s):  
Isao Oishi ◽  
Kenji Yamazaki ◽  
Tatsuo Kimoto ◽  
Yoshiichi Minekawa
Keyword(s):  
Molecular Weight ◽  
Immunoblot Analysis ◽  
Low Molecular Weight ◽  
Western Immunoblot ◽  
Western Immunoblot Analysis

scholarly journals Phosphorylation of nuclear and flagellar basal apparatus proteins during flagellar regeneration in Chlamydomonas reinhardtii

1993 ◽  
Vol 122 (4) ◽  
pp. 877-886 ◽  
Author(s):  
JD Harper ◽  
MA Sanders ◽  
JL Salisbury
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Transcriptional Activation ◽  
Kinase Inhibitor ◽  
Nuclear Periphery ◽  
Immunoblot Analysis ◽  
Basal Region ◽  
Western Immunoblot ◽  
Flagellar Regeneration ◽  
Basal Apparatus ◽  
Western Immunoblot Analysis

The antiphosphoprotein monoclonal antibody MPM-2 was used to investigate protein phosphorylation during flagellar regeneration in Chlamydomonas reinhardtii. MPM-2 recognizes a phosphorylated epitope and detects several Chlamydomonas proteins by Western immunoblot analysis. Two MPM-2 reactive proteins (34 and 90 kD) increase in Western immunoblot intensity after flagellar excision and decrease in intensity during flagellar regeneration. Immunofluorescence and immunogold labeling revealed MPM-2 staining within the nucleus, especially towards the nuclear periphery, the flagellar basal apparatus, and the nucleus-basal body connector after flagellar excision. Comparison of MPM-2 reactivity in wild-type cells and in the mutant bald-2, which lacks functional basal bodies, demonstrates that the 34-kD protein is localized in the nucleus and the 90-kD protein is localized in the flagellar basal region. MPM-2 reactivity is observed in cells competent for flagellar regeneration. However, when cells were treated with the kinase inhibitor, staurosporine, MPM-2 reactivity did not increase after flagellar excision and flagellar regeneration was impaired. These observations suggest that phosphorylation of the 34- and 90-kD proteins may be important for flagellar regrowth. Possible roles for phosphorylation in flagellar regeneration include transcriptional activation and transport of flagellar precursors to the base of the growing flagella.

scholarly journals Análise antigênica e molecular de amostras citopáticas do vírus da diarréia viral bovina

2000 ◽  
Vol 30 (1) ◽  
pp. 129-135 ◽  
Author(s):  
Fernando Luiz Tobias ◽  
Anselmo Odeon ◽  
Edwiges Maristela Pituco ◽  
Rudi Weiblen ◽  
Dino César Garcez ◽  
...  
Keyword(s):  
Western Immunoblot

Sete amostras citopáticas do vírus da Diarréia Viral Bovina (BVDV) isoladas de casos clínicos e do sangue de bezerros de rebanhos com problemas reprodutivos foram analisadas. Todas as amostras caracterizadas possuíam uma mistura de vírus citopáticos (cp) e não-citopáticos (ncp), que foram clonados biologicamente, originando populações puras de vírus de cada biotipo. Os clones cp e ncp obtidos foram caracterizados antigenicamente com um painel de anticorpos monoclonais (MAbs) e quanto à expressão da proteína não-estrutural NS3. A análise de reconhecimento pelos MAbs revelou dois padrões de reatividade: 1. Em cinco casos, os vírus cp e ncp de uma mesma amostra mostraram-se antigenicamente muito semelhantes entre si, indicando tratar-se de verdadeiros "pares" de vírus, nos quais o vírus cp origina-se do ncp através de mutações ou recombinações; 2. Duas amostras, no entanto, continham vírus cp e ncp com diferenças antigênicas consideráveis entre si. A análise de polipeptídeos não-estruturais das amostras ncp através de Western immunoblot revelou uma única banda de reatividade, de massa aproximada de 125kDa, correspondente à proteína nãoestrutural NS23. As amostras cp expressaram, além da NS23, um polipeptídeo de massa aproximada de 80kDa, correspondente à NS3. Duas amostras cp apresentaram diferenças na migração da NS23. Uma amostra apresentou a NS23 com massa menor do que 125kDa, enquanto outra amostra apresentou duas bandas de reatividade, com massas menor e maior que a NS23 dos demais vírus, respectivamente. Esses resultados confirmam achados anteriores de que amostras de campo citopáticas do BVDV geralmente possuem vírus dos dois biotipos e que o fenótipo citopático está associado à expressão da proteína NS3. O isolamento de amostras citopáticas do sangue de animais clinicamente normais e de um feto, no entanto, demonstra que a ocorrência de vírus cp não se restringe à casos da Doença das Mucosas.

Serodiagnosis of Lyme borreliosis by western immunoblot: reactivity of various significant antibodies against Borrelia burgdorferi.

1992 ◽  
Vol 30 (2) ◽  
pp. 370-376 ◽  
Author(s):  
B Ma ◽  
B Christen ◽  
D Leung ◽  
C Vigo-Pelfrey
Keyword(s):  
Borrelia Burgdorferi ◽  
Lyme Borreliosis ◽  
Western Immunoblot

Western Immunoblot Analysis

1998 ◽  
pp. 295-306 ◽  
Author(s):  
Cynthia E. Shaw ◽  
Jing Zheng
Keyword(s):  
Immunoblot Analysis ◽  
Western Immunoblot ◽  
Western Immunoblot Analysis

High prevalence of human T-lymphotropic virus type I infection in isolated populations of the Western Pacific region confirmed by Western immunoblot

1990 ◽  
Vol 2 (4) ◽  
pp. 439-447 ◽  
Author(s):  
R. M. Garruto ◽  
M. Slover ◽  
R. Yanagihara ◽  
C. A. Mora ◽  
S. S. Alexander ◽  
...  
Keyword(s):  
Virus Type ◽  
Western Pacific ◽  
Type I ◽  
Pacific Region ◽  
Western Pacific Region ◽  
Isolated Populations ◽  
T Lymphotropic Virus ◽  
Western Immunoblot ◽  
High Prevalence ◽  
The Western Pacific
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