scholarly journals Detection of human enteric viruses in shellfish, vegetables, waters and environmental samples: a preliminary study

2010 ◽  
Vol 14 ◽  
pp. e63-e64
Author(s):  
V. Cannella ◽  
G. Purpari ◽  
A. Ferrari ◽  
A. Migliazzo ◽  
P. Di Marco ◽  
...  
Keyword(s):  
Environmental Samples ◽  
Enteric Viruses ◽  
Preliminary Study ◽  
Human Enteric Viruses

Evaluation of a novel electropositive filter for the concentration of viruses from diverse water matrices

2010 ◽  
Vol 61 (2) ◽  
pp. 317-322 ◽  
Author(s):  
H. B. Bennett ◽  
H. D. O'Dell ◽  
G. Norton ◽  
G. Shin ◽  
F.-C. Hsu ◽  
...  
Keyword(s):  
Surface Water ◽  
Environmental Samples ◽  
Ground Surface ◽  
Enteric Viruses ◽  
Artificial Seawater ◽  
The Novel ◽  
Environmental Waters ◽  
Ionic Concentrations ◽  
The Mean ◽  
Human Enteric Viruses

Human enteric viruses are important agents of waterborne illness. They are diffusely distributed in environmental waters, necessitating concentration of tens to hundreds of litres for effective detection. This study evaluates the novel ViroCap disposable capsule filter for concentration of coliphage MS2 and poliovirus (PV1) from deionised (DI) water and artificial seawater, as well as natural ground, surface, and seawater. Retention and recoveries for the ViroCap were compared with two well-characterised filters: the 1MDS for DI water, and the OptiCap XL for artificial seawater. The mean adsorption for MS2 by the ViroCap was 88%. Recovery of MS2 was significantly greater (p ≤ 0.01) than alternative filters tested: 65% from DI water and 63% from artificial seawater, compared to 30% for the 1MDS and 15% for the OptiCap for the respective matrices. Recovery of PV1 from DI water (37%) was similar to that of the 1MDS (51%). PV1 recoveries from artificial seawater were significantly greater (p ≤ 0.01) for the ViroCap (44%) than the OptiCap (11%). Recovery of MS2 from seeded environmental samples yielded 44% from groundwater, 53% from surface water, and 51% from seawater. ViroCap disposable filter is efficient for concentrating MS2 and PV1 from diverse matrices and is robust across a range of ionic concentrations.

scholarly journals Evaluation of Two Triplex One-Step qRT-PCR Assays for the Quantification of Human Enteric Viruses in Environmental Samples

2017 ◽  
Vol 9 (3) ◽  
pp. 342-349 ◽  
Author(s):  
Kata Farkas ◽  
Dafydd E. Peters ◽  
James E. McDonald ◽  
Alexis de Rougemont ◽  
Shelagh K. Malham ◽  
...  
Keyword(s):  
Environmental Samples ◽  
Enteric Viruses ◽  
Qrt Pcr ◽  
One Step ◽  
Pcr Assays ◽  
Human Enteric Viruses

scholarly journals Evaluation of Human Enteric Viruses in Surface Water and Drinking Water Resources in Southern Ghana

2011 ◽  
Vol 84 (1) ◽  
pp. 20-29 ◽  
Author(s):  
Kristen E. Gibson ◽  
Yayi Guo ◽  
James T. Schissler ◽  
Melissa C. Opryszko ◽  
Kellogg J. Schwab
Keyword(s):  
Drinking Water ◽  
Surface Water ◽  
Water Resources ◽  
Enteric Viruses ◽  
Drinking Water Resources ◽  
Human Enteric Viruses ◽  
Southern Ghana

Virus occurrence in municipal groundwater sources in Quebec, Canada

2007 ◽  
Vol 53 (6) ◽  
pp. 688-694 ◽  
Author(s):  
Annie Locas ◽  
Christine Barthe ◽  
Benoit Barbeau ◽  
Annie Carrière ◽  
Pierre Payment
Keyword(s):  
Drinking Water ◽  
Sampling Site ◽  
Enteric Viruses ◽  
Fecal Coliforms ◽  
Coliform Bacteria ◽  
Raw Water ◽  
Total Coliforms ◽  
Two Samples ◽  
Historic Data ◽  
Human Enteric Viruses

A 1 year study was undertaken on groundwater that was a source of drinking water in the province of Quebec, Canada. Twelve municipal wells (raw water) were sampled monthly during a 1 year period, for a total of 160 samples. Using historic data, the 12 sites were categorized into 3 groups: group A (no known contamination), group B (sporadically contaminated by total coliforms), and group C (historic and continuous contamination by total coliforms and (or) fecal coliforms). Bacterial indicators (total coliform, Escherichia coli , enteroccoci), viral indicators (somatic and male-specific coliphages), total culturable human enteric viruses, and noroviruses were analyzed at every sampling site. Total coliforms were the best indicator of microbial degradation, and coliform bacteria were always present at the same time as human enteric viruses. Two samples contained human enteric viruses but no fecal pollution indicators (E. coli, enterococci, or coliphages), suggesting the limited value of these microorganisms in predicting the presence of human enteric viruses in groundwater. Our results underline the value of historic data in assessing the vulnerability of a well on the basis of raw water quality and in detecting degradation of the source. This project allowed us to characterize the microbiologic and virologic quality of groundwater used as municipal drinking water sources in Quebec.

Detection of Human Enteric Viruses in French Polynesian Wastewaters, Environmental Waters and Giant Clams

2018 ◽  
Vol 11 (1) ◽  
pp. 52-64 ◽  
Author(s):  
Laetitia Kaas ◽  
Leslie Ogorzaly ◽  
Gaël Lecellier ◽  
Véronique Berteaux-Lecellier ◽  
Henry-Michel Cauchie ◽  
...  
Keyword(s):  
Enteric Viruses ◽  
Environmental Waters ◽  
Giant Clams ◽  
Human Enteric Viruses

scholarly journals Evaluation of Adenovirus andE. colias indicators for human enteric viruses presence in mussels produced in La Spezia Gulf (Italy)

2010 ◽  
Vol 50 (5) ◽  
pp. 462-467 ◽  
Author(s):  
L. Serracca ◽  
M. Verani ◽  
R. Battistini ◽  
I. Rossini ◽  
A. Carducci ◽  
...  
Keyword(s):  
Enteric Viruses ◽  
Human Enteric Viruses

scholarly journals Three-Year Study To Assess Human Enteric Viruses in Shellfish

2000 ◽  
Vol 66 (8) ◽  
pp. 3241-3248 ◽  
Author(s):  
F. Le Guyader ◽  
L. Haugarreau ◽  
L. Miossec ◽  
E. Dubois ◽  
M. Pommepuy
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Detection ◽  
Enteric Viruses ◽  
Molecular Technique ◽  
Norwalk Virus ◽  
Viral Contamination ◽  
Long Period ◽  
Reverse Transcription Pcr ◽  
Human Enteric Viruses

ABSTRACT The main pathogenic enteric viruses able to persist in the environment, such as hepatitis A virus (HAV), Norwalk-like virus (NLV), enterovirus (EV), rotavirus (RV), and astrovirus (AV), were detected by reverse transcription-PCR and hybridization in shellfish during a 3-year study. Oyster samples (n = 108), occasionally containing bacteria, were less frequently contaminated, showing positivity for AV (17%), NLV (23%), EV (19%), and RV (27%), whereas mussel samples, collected in areas routinely impacted by human sewage, were more highly contaminated: AV (50%), HAV (13%), NLV (35%), EV (45%), and RV (52%). Sequences obtained from HAV and NLV amplicons showed a great variety of strains, especially for NLV (strains close to Mexico, Snow Mountain Agent, or Norwalk virus). Viral contamination was mainly observed during winter months, although there were some seasonal differences among the viruses. This first study of virus detection over a fairly long period of time suggests that routine analysis of shellfish by a molecular technique is feasible.

scholarly journals Seasonal and diurnal surveillance of treated and untreated wastewater for human enteric viruses

2018 ◽  
Vol 25 (33) ◽  
pp. 33391-33401 ◽  
Author(s):  
Kata Farkas ◽  
Miles Marshall ◽  
David Cooper ◽  
James E. McDonald ◽  
Shelagh K. Malham ◽  
...  
Keyword(s):  
Enteric Viruses ◽  
Untreated Wastewater ◽  
Human Enteric Viruses
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