scholarly journals High-throughput bead based suspension array for the detection of acute respiratory viral pathogens among children aged <5 years in Pakistan

2016 ◽  
Vol 53 ◽  
pp. 100-101
Author(s):  
F. Aziz ◽  
J. Samad ◽  
I. Rizvi ◽  
A. Sami ◽  
S. Qureshi ◽  
...  
Keyword(s):  
High Throughput ◽  
Viral Pathogens ◽  
Suspension Array

scholarly journals Combination of Suspension Array and Mycelial Growth Assay for Detecting Multiple-Fungicide Resistance in Botrytis cinerea in Hebei Province in China

Plant Disease ◽  
2019 ◽  
Vol 103 (6) ◽  
pp. 1213-1219
Author(s):  
Zehua Su ◽  
Xin Zhang ◽  
Jianjiang Zhao ◽  
Wenqiao Wang ◽  
Lei Shang ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
High Throughput ◽  
Fungicide Resistance ◽  
Mycelial Growth ◽  
Accurate Method ◽  
Hebei Province ◽  
Combination Method ◽  
Major Type ◽  
Suspension Array ◽  
Growth Assay

To provide a high-throughput, efficient, and accurate method to monitor multiple-fungicide resistance of Botrytis cinerea in the field, we used the suspension array, sequencing, and mycelial growth assay in our research. Discriminating-dose bioassays for detecting carbendazim, diethofencarb, boscalid, and iprodione resistance (CarR, DieR, BosR, and IprR, respectively) were used to analyze 257 isolates collected from Hebei Province in China during 2016 and 2017. High resistance frequencies to carbendazim (100%), diethofencarb (92.08%), and iprodione (86.59%) were detected. BosR isolates accounted for 11.67% of the total. In addition, 103 isolates were randomly selected for phenotype and genotype detection. The high-throughput suspension array was utilized to detect eight genotypes simultaneously, including BenA-E198, BenA-198A, SdhB-H272, SdhB-272Y, BcOS1-I365, BcOS1-365S, erg27-F412, and erg27-412S, which were associated with resistance toward carbendazim or diethofencarb, boscalid, iprodione, and fenhexamid (FenR), respectively. Most of the benzimidazole-resistant isolates (81.55%) possessed the E198V mutation in the BenA gene. Ninety-three isolates with dual resistance to carbendazim and diethofencarb showed the E198V/K mutation. All BosR isolates carried the H272R mutation in the SdhB gene. The I365S and Q369P+N373S (66.99%) mutations in the BcOS1 gene were more frequently observed. No mutation was detected in the erg27 gene in Hebei isolates. There were 13 resistance profile phenotypes. Phenotypes with triple resistance were the most common (83.50%), and CarRDieRBosSIprRFenS was the major type. CarR isolates that carried E198V/K/A were all highly resistant (HR) and only one F200Y mutant was moderately resistant (MR) to carbendazim. Isolates that possessed E198V/K were MR or HR to diethofencarb. BosR isolates that possessed H272R mutation were lowly resistant (LR). IprR isolates were all LR or MR. The distribution of half maximal effective concentrations of CarR isolates with E198V/K mutations and IprR isolates with Q369P+N373S mutations significantly increased from 2016 to 2017. Combined with our observations, a combination method of the high-throughput suspension array and the mycelial growth assay was suggested to accurately monitor multiple resistance of B. cinerea in the field.

Simultaneous detection of five antibiotics in milk by high-throughput suspension array technology

Talanta ◽  
2011 ◽  
Vol 85 (2) ◽  
pp. 1160-1165 ◽  
Author(s):  
Pu Su ◽  
Nan Liu ◽  
Maoxiang Zhu ◽  
Baoan Ning ◽  
Ming Liu ◽  
...  
Keyword(s):  
High Throughput ◽  
Simultaneous Detection ◽  
Suspension Array ◽  
Array Technology

scholarly journals Dual-spectra encoded suspension array using reversed-phase microemulsion UV curing and electrostatic self-assembling

RSC Advances ◽  
2018 ◽  
Vol 8 (38) ◽  
pp. 21272-21279 ◽  
Author(s):  
Guangxia Feng ◽  
Qinghua He ◽  
WenYue Xie ◽  
Yonghong He ◽  
Xuejing Chen ◽  
...  
Keyword(s):  
High Throughput ◽  
Rapid Growth ◽  
Uv Curing ◽  
Reversed Phase ◽  
Suspension Array ◽  
Self Assembling ◽  
Modern Biotechnology

The rapid growth of demand for high-throughput multiplexed biochips from modern biotechnology has led to growing interest in suspension array based on multi-channel encoded microbeads.

scholarly journals Rice Stripe Tenuivirus Has a Greater Tendency To Use the Prime-and-Realign Mechanism in Transcription of Genomic than in Transcription of Antigenomic Template RNAs

2017 ◽  
Vol 92 (1) ◽  
Author(s):  
Xiaojuan Liu ◽  
Jing Jin ◽  
Ping Qiu ◽  
Fangluan Gao ◽  
Wenzhong Lin ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Rna Viruses ◽  
Viral Mrnas ◽  
Viral Pathogens ◽  
Promising Target ◽  
Cellular Mrnas ◽  
Sequencing Strategy ◽  
First Time ◽  
Negative Sense

ABSTRACTMost segmented negative-sense RNA viruses employ a process termed cap snatching, during which they snatch capped RNA leaders from host cellular mRNAs and use the snatched leaders as primers for transcription, leading to the synthesis of viral mRNAs with 5′ heterogeneous sequences (HSs). With traditional methods, only a few HSs can be determined, and identification of their donors is difficult. Here, the mRNA 5′ ends ofRice stripe tenuivirus(RSV) andRice grassy stunt tenuivirus(RGSV) and those of their host rice were determined by high-throughput sequencing. Millions of tenuiviral HSs were obtained, and a large number of them mapped to the 5′ ends of corresponding host cellular mRNAs. Repeats of the dinucleotide AC, which are complementary to the U1G2of the tenuiviral template 3′-U1G2U3G4UUUCG, were found to be prevalent at the 3′ termini of tenuiviral HSs. Most of these ACs did not match host cellular mRNAs, supporting the idea that tenuiviruses use the prime-and-realign mechanism during cap snatching. We previously reported a greater tendency of RSV than RGSV to use the prime-and-realign mechanism in transcription with leaders cap snatched from a coinfecting reovirus. Besides confirming this observation in natural tenuiviral infections, the data here additionally reveal that RSV has a greater tendency to use this mechanism in transcribing genomic than in transcribing antigenomic templates. The data also suggest that tenuiviruses cap snatch host cellular mRNAs from translation- and photosynthesis-related genes, and capped RNA leaders snatched by tenuiviruses base pair with U1/U3or G2/G4of viral templates. These results provide unprecedented insights into the cap-snatching process of tenuiviruses.IMPORTANCEMany segmented negative-sense RNA viruses (segmented NSVs) are medically or agriculturally important pathogens. The cap-snatching process is a promising target for the development of antiviral strategies against this group of viruses. However, many details of this process remain poorly characterized. Tenuiviruses constitute a genus of agriculturally important segmented NSVs, several members of which are major viral pathogens of rice. Here, we for the first time adopted a high-throughput sequencing strategy to determine the 5′ heterogeneous sequences (HSs) of tenuiviruses and mapped them to host cellular mRNAs. Besides providing deep insights into the cap snatching of tenuiviruses, the data obtained provide clear evidence to support several previously proposed models regarding cap snatching. Curiously and importantly, the data here reveal that not only different tenuiviruses but also the same tenuivirus synthesizing different mRNAs use the prime-and-realign mechanism with different tendencies during their cap snatching.

High-throughput simultaneous genotyping of human platelet antigen-1 to -16 by using suspension array

Transfusion ◽  
2013 ◽  
Vol 53 (11) ◽  
pp. 2722-2728 ◽  
Author(s):  
Qun-Xing An ◽  
Cui-Ying Li ◽  
Li-Juan Xu ◽  
Xian-Qing Zhang ◽  
Yan-Jun Bai ◽  
...  
Keyword(s):  
High Throughput ◽  
Human Platelet ◽  
Suspension Array ◽  
Platelet Antigen ◽  
Human Platelet Antigen

scholarly journals Target-independent high-throughput sequencing methods provide evidence that already known human viral pathogens play a main role in respiratory infections with unexplained etiology

2019 ◽  
Vol 8 (1) ◽  
pp. 1054-1065 ◽  
Author(s):  
Unai Pérez-Sautu ◽  
Michael Ross Wiley ◽  
María Iglesias-Caballero ◽  
Francisco Pozo ◽  
Karla Prieto ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Respiratory Infections ◽  
Main Role ◽  
Viral Pathogens

Direct Metagenomic Detection of Viral Pathogens in Human Specimens Using an Unbiased High-Throughput Sequencing Approach

2011 ◽  
pp. 73-80 ◽  
Author(s):  
Takaaki Nakaya ◽  
Shota Nakamura ◽  
Yoshiko Okamoto ◽  
Yoshiyuki Nagai ◽  
Jun Kawai ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Viral Pathogens

Cytometric Microbead Magnetic Suspension Array for High-Throughput Ultrasensitive Detection of Aflatoxin B1

2018 ◽  
Vol 91 (1) ◽  
pp. 1194-1202 ◽  
Author(s):  
Xiaofei Liu ◽  
Guangyao Ying ◽  
Xiaofang Liao ◽  
Chaonan Sun ◽  
Fang Wei ◽  
...  
Keyword(s):  
High Throughput ◽  
Aflatoxin B1 ◽  
Magnetic Suspension ◽  
Ultrasensitive Detection ◽  
Suspension Array

scholarly journals A high-throughput and ultrasensitive identification methodology for unauthorized GMP component based on suspension array and logical calculator

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Pengyu Zhu ◽  
Wei Fu ◽  
Shuang Wei ◽  
Xiao Liu ◽  
Chenguang Wang ◽  
...  
Keyword(s):  
High Throughput ◽  
Suspension Array

scholarly journals A Comparative High-Throughput Screening Protocol to Identify Entry Inhibitors of Enveloped Viruses

2013 ◽  
Vol 19 (1) ◽  
pp. 100-107 ◽  
Author(s):  
Juan Wang ◽  
Han Cheng ◽  
Kiira Ratia ◽  
Elizabeth Varhegyi ◽  
William G. Hendrickson ◽  
...  
Keyword(s):  
High Throughput ◽  
Viral Entry ◽  
High Throughput Screening ◽  
Marburg Virus ◽  
Lassa Virus ◽  
Viral Pathogens ◽  
Enveloped Viruses ◽  
Entry Inhibitors ◽  
Screening Protocol ◽  
Therapeutic Prevention

Emerging and reemerging human viral pathogens pose great public health concerns since therapeutics against these viruses are limited. Thus, there is an urgent need to develop novel drugs that can block infection of either a specific virus or a number of viruses. Viral entry is thought to be an ideal target for potential therapeutic prevention. One of the challenges of developing antivirals is that most of these viruses are highly pathogenic and therefore require high biosafety-level containment. In this study, we have adopted a comparative high-throughput screening protocol to identify entry inhibitors for three enveloped viruses (Marburg virus, influenza virus H5N1, and Lassa virus) using a human immunodeficiency virus–based pseudotyping platform. We demonstrate the utility of this approach by screening a small compound library and identifying putative entry inhibitors for these viruses. One major advantage of this protocol is to reduce the number of false positives in hit selection, and we believe that the protocol is useful for inhibitor screening for many enveloped viruses.

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