scholarly journals Methicillin-resistant coagulase positive Staphylococcus aureus and Staphylococcus pseudintermedius circulating in dogs in Bangladesh

2020 ◽  
Vol 101 ◽  
pp. 34-35
Author(s):  
E.A. Rana ◽  
M.Z. Islam ◽  
T. Das ◽  
A. Dutta ◽  
A. Ahad ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Coagulase Positive Staphylococcus

scholarly journals Occurrence and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Methicillin-Resistant Staphylococcus pseudintermedius in an Academic Veterinary Hospital

2010 ◽  
Vol 76 (15) ◽  
pp. 5165-5174 ◽  
Author(s):  
Kanako Ishihara ◽  
Natsumi Shimokubo ◽  
Akie Sakagami ◽  
Hiroshi Ueno ◽  
Yasukazu Muramatsu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Control Strategies ◽  
Companion Animals ◽  
Molecular Characteristics ◽  
Stepwise Logistic Regression ◽  
Type Ii ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Type Iv ◽  
Veterinary Hospital

ABSTRACT Recently, methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) have been increasingly isolated from veterinarians and companion animals. With a view to preventing the spread of MRSA and MRSP, we evaluated the occurrence and molecular characteristics of each in a veterinary college. MRSA and MRSP were isolated from nasal samples from veterinarians, staff members, and veterinary students affiliated with a veterinary hospital. Using stepwise logistic regression, we identified two factors associated with MRSA carriage: (i) contact with an identified animal MRSA case (odds ratio [OR], 6.9; 95% confidence interval [95% CI], 2.2 to 21.6) and (ii) being an employee (OR, 6.2; 95% CI, 2.0 to 19.4). The majority of MRSA isolates obtained from individuals affiliated with the veterinary hospital and dog patients harbored spa type t002 and a type II staphylococcal cassette chromosome mec (SCCmec), similar to the hospital-acquired MRSA isolates in Japan. MRSA isolates harboring spa type t008 and a type IV SCCmec were obtained from one veterinarian on three different sampling occasions and also from dog patients. MRSA carriers can also be a source of MRSA infection in animals. The majority of MRSP isolates (85.2%) carried hybrid SCCmec type II-III, and almost all the remaining MRSP isolates (11.1%) carried SCCmec type V. MRSA and MRSP were also isolated from environmental samples collected from the veterinary hospital (5.1% and 6.4%, respectively). The application of certain disinfection procedures is important for the prevention of nosocomial infection, and MRSA and MRSP infection control strategies should be adopted in veterinary medical practice.

scholarly journals Characterization of Staphylococcal Cassette Chromosome mec Elements from Methicillin-Resistant Staphylococcus pseudintermedius Infections in Australian Animals

mSphere ◽  
2018 ◽  
Vol 3 (6) ◽  
Author(s):  
Kate A. Worthing ◽  
Sybille Schwendener ◽  
Vincent Perreten ◽  
Sugiyono Saputra ◽  
Geoffrey W. Coombs ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genomic Structure ◽  
Cadmium Resistance ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Content Type ◽  
Resistance Determinants ◽  
Oxacillin Resistance ◽  
Animal Pathogens ◽  
Staphylococcal Cassette Chromosome

ABSTRACT We examined the oxacillin resistance phenotype and genomic structure of staphylococcal cassette chromosome mec (SCCmec) elements from 77 veterinary methicillin-resistant Staphylococcus pseudintermedius (MRSP) isolates. Isolates were characterized by oxacillin broth microdilution, whole-genome sequencing, and bioformatics analysis. Five previously described SCCmec elements, and a sixth novel element, were identified: SCCmec III (also known as II-III), ΨSCCmec57395, and SCCmecNA45 (a SCCmec VII variant), all previously described in MRSP, and SCCmec IVg and SCCmec VT, previously described in both methicillin-resistant Staphylococcus aureus (MRSA) and MRSP. The sixth element was novel and found among nine geographically clustered isolates. This novel pseudostaphylococcal cassette chromosome (ΨSCCmecKW21) contained a class A mec gene complex but lacked ccr genes. It also harbored heavy metal (cadmium) resistance determinants. The median oxacillin MIC values among ΨSCCmecKW21, SCCmec III, and SCCmec VT isolates were significantly higher than those determined for the SCCmecNA45 VII variant isolates and ΨSCCmec57395 and SCCmec IVg isolates. ΨSCCmecKW21 was found exclusively in sequence type 497 (ST497), an MRSP clone that is locally successful in Victoria, Australia. Future studies are necessary to determine if this clone has disseminated further afield and if ΨSCCmecKW21 has moved into other MRSP lineages or staphylococcal species. IMPORTANCE Staphylococcus pseudintermedius is a significant veterinary pathogen and occasional cause of infections in humans. β-Lactams are an important group of antimicrobials used to treat staphylococcal infections in humans and animals. However, when staphylococci become methicillin resistant via the acquisition of a mobile genetic element called staphylococcal cassette chromosome mec (SCCmec), they become resistant to all β-lactams. This study detected a novel SCCmec element among a cluster of methicillin-resistant S. pseudintermedius isolates from animals in Australia. It also detected SCCmec elements in S. pseudintermedius that had high similarity to those identified in methicillin-resistant Staphylococcus aureus, demonstrating how human and animal pathogens can share the same resistance determinants.

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