Design of a reduced objective Lens fluorescence dPCR Gene chip detection system with high-throughput and large field of view

Optik ◽  
2019 ◽  
Vol 179 ◽  
pp. 1071-1083
Author(s):  
Francis Yaw Otuboah ◽  
Zheng Jihong ◽  
Zhu Tianyun ◽  
Chen Cheng
Keyword(s):  
High Throughput ◽  
Detection System ◽  
Gene Chip ◽  
Field Of View ◽  
Large Field ◽  
Objective Lens

High-throughput and uniform large field-of-view multichannel fluorescence microscopy with super-thin dichroism for a dPCR gene chip

2020 ◽  
Vol 59 (34) ◽  
pp. 10768
Author(s):  
Francis Yaw Otuboah ◽  
Jihong Zheng ◽  
Cheng Chen ◽  
Zicheng Wang ◽  
Xinjun Wan ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
High Throughput ◽  
Gene Chip ◽  
Field Of View ◽  
Large Field

scholarly journals High-throughput characterisation of bull semen motility using differential dynamic microscopy

2018 ◽  
Author(s):  
Alys Jepson ◽  
Jochen Arlt ◽  
Jonathan Statham ◽  
Mark Spilman ◽  
Katie Burton ◽  
...  
Keyword(s):  
High Throughput ◽  
Oscillation Amplitude ◽  
Field Of View ◽  
Large Field ◽  
Laboratory Measurements ◽  
Spermatozoa Motility ◽  
Bull Semen ◽  
The Mean ◽  
On Farm ◽  
Low Cell Density

AbstractWe report a high-throughput technique for characterising the motility of spermatozoa using differential dynamic microscopy. A large field of view movie (~ 10mm2) records thousands of cells (e.g. ≈ 5000 cells even at a low cell density of 20 × 106 cells/ml) at once and yields averaged measurements of the mean (υ) and standard deviation (σ) of the swimming speed, a head oscillation amplitude (A0) and frequency (f0), and the fraction of motile spermatozoa (α). Interestingly, the measurement of α relies on the swimming spermatozoa enhancing the motion of the non-swimming population. We demonstrate the ease and rapidity of our method by performing on-farm characterisation of bull spermatozoa motility, and validate the technique by comparing laboratory measurements with tracking. Our results confirm the long-standing theoretical prediction that for swimming spermatozoa.

Freeform-Surface-Based Optical Detection System with Large Relative Aperture and Large Field of View

2020 ◽  
Vol 40 (17) ◽  
pp. 1722002
Author(s):  
马子轩 Ma Zixuan ◽  
李旭阳 Li Xuyang ◽  
任志广 Ren Zhiguang ◽  
褚楠清 Chu Nanqing
Keyword(s):  
Detection System ◽  
Optical Detection ◽  
Field Of View ◽  
Freeform Surface ◽  
Large Field ◽  
Optical Detection System

Apochromatic Collimator Objective Lens Design with Big Field of View

2013 ◽  
Vol 710 ◽  
pp. 413-418
Author(s):  
Fei Guo ◽  
Mei Zhao ◽  
Mai Yu Zhou ◽  
Ming Quan Yang ◽  
Shan Shan Cong
Keyword(s):  
Image Quality ◽  
Structural Parameters ◽  
Optical Design ◽  
Field Of View ◽  
Practical Significance ◽  
Large Field ◽  
Objective Lens ◽  
Initial Structure ◽  
Core Part

Objective lens is the core part of a collimator, its quality has a direct impact on the collimators quality. Due to the small field of view and low image quality requirement, general collimator objective lens is difficult to achieve the requirement of testing an aerial camera s performance. So designing a large field of view, apochromatic collimator objective lens has an important practical significance. In this paper, the major qualifications were first proposed to design the collimator objective lens,according to which the initial configuration was confirmed. Then we used the ZEMAX optical design software to optimize the initial structure and normalized the optimized structural parameters. Finally the image quality of collimator objective lens was evaluated to make it achieve the requirement of qualifications.

Large Field of View Space-Based Optical Detection System Based on Freeform Surfaces

2018 ◽  
Vol 38 (11) ◽  
pp. 1122003
Author(s):  
倪栋伟 Ni Dongwei ◽  
李旭阳 Li Xuyang ◽  
杨明洋 Yang Mingyang ◽  
任志广 Ren Zhiguang
Keyword(s):  
Detection System ◽  
Optical Detection ◽  
Field Of View ◽  
Large Field ◽  
Freeform Surfaces ◽  
Optical Detection System

Design of ultra-compact optical detection system with large field of view

2020 ◽  
Vol 28 (12) ◽  
pp. 2581-2587
Author(s):  
Zi-xuan MA ◽  
◽  
Xu-yang LI ◽  
Zhi-guang REN ◽  
Nan-qing CHU ◽  
...  
Keyword(s):  
Detection System ◽  
Optical Detection ◽  
Field Of View ◽  
Large Field ◽  
Optical Detection System

Multiple beam ptychography for large field-of-view, high throughput, quantitative phase contrast imaging

2018 ◽  
Vol 184 ◽  
pp. 164-171 ◽  
Author(s):  
Charles Bevis ◽  
Robert Karl ◽  
Jonathan Reichanadter ◽  
Dennis F. Gardner ◽  
Christina Porter ◽  
...  
Keyword(s):  
High Throughput ◽  
Phase Contrast ◽  
Field Of View ◽  
Phase Contrast Imaging ◽  
Large Field ◽  
Contrast Imaging ◽  
Quantitative Phase ◽  
Multiple Beam

Electron holography of p-n junctions

1996 ◽  
Vol 54 ◽  
pp. 974-975
Author(s):  
B.G. Frost ◽  
D.C. Joy ◽  
L.F. Allard ◽  
E. Voelkl
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
Field Emission ◽  
Ccd Camera ◽  
Image Plane ◽  
Reference Wave ◽  
Field Of View ◽  
Control Mode ◽  
Objective Lens ◽  
Electron Holography

A wide holographic field of view (up to 15 μm in the Hitachi-HF2000) is achieved in a TEM by switching off the objective lens and imaging the sample by the first intermediate lens. Fig.1 shows the corresponding ray diagram for low magnification image plane off-axis holography. A coherent electron beam modulated by the sample in its amplitude and its phase is superimposed on a plane reference wave by a negatively biased Möllenstedt-type biprism.Our holograms are acquired utilizing a Hitachi HF-2000 field emission electron microscope at 200 kV. Essential for holography are a field emission gun and an electron biprism. At low magnification, the excitation of each lens must be appropriately adjusted by the free lens control mode of the microscope. The holograms are acquired by a 1024 by 1024 slow-scan CCD-camera and processed by the “Holoworks” software. The hologram fringes indicate positively and negatively charged areas in a sample by the direction of the fringe bending (Fig.2).

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