Detection of Helicobacter pylori in adenotonsillar tissue of children with chronic adenotonsillitis using rapid urease test, PCR and blood serology: A prospective study

2011 ◽  
Vol 75 (4) ◽  
pp. 568-572 ◽  
Author(s):  
Mohamed H. Abdel-Monem ◽  
Emad A. Magdy ◽  
Yasser A. Nour ◽  
Reem A. Harfoush ◽  
Alnagy Ibreak
Keyword(s):  
Helicobacter Pylori ◽  
Prospective Study ◽  
Rapid Urease Test ◽  
Urease Test ◽  
A Prospective Study

scholarly journals Assessment of medication adherence in Helicobacter pylori positive patients on standard triple therapy: a prospective study

2020 ◽  
Vol 9 (7) ◽  
pp. 1100
Author(s):  
Aleena Francis ◽  
Arathi S. Nair ◽  
Limi Joseph Gomez ◽  
Bushra Abdul Rahim ◽  
Soumya R. V. ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Medication Adherence ◽  
Prospective Study ◽  
Triple Therapy ◽  
Study Patient ◽  
Rapid Urease Test ◽  
Adherence Score ◽  
Standard Triple Therapy ◽  
Urease Test ◽  
A Prospective Study

Background: In the current study patient compliance to the standard triple therapy were assessed. The objective behind this research was to assess the patient medication adherence to the standard triple therapy in Helicobacter pylori infection.Methods: A prospective study was carried out for a period of 1 year and samples were taken from the gastroenterology department. Patient who was RUT (rapid urease test) positive by endoscopy were considered as H. pylori infected and they were prescribed with standard triple therapy. This regimen involves amoxicillin 1000 mg and clarithromycin 500 mg and proton pump inhibitor (PPI) twice a day for 14 days. Adherence to this triple therapy was assessed during the study. Medication adherence assessed using Morisky, Green and Levine (MGL) adherence scale. MGL adherence questionnaire was given to patients during first week and second week of therapy. The patients will be counselled regarding the drug administration, drug related problems and the infection. They were also provided with written instructions in leaflets. 88 patients were analyzed.Results: In this study, 84 patients had a good adherence 95.5%; but other 4.5% didn't fully comply with the physician's order. After the first week of standard triple therapy, adherence was increased to a mean score from 2.193±0.1301 to 3.5227±0.0704 in the second week. The medication adherence score significantly increased along with patient counselling.Conclusions: Medication adherence was improved in the 14 days course along with patient education helped to comply with the standard triple therapy.

scholarly journals Helicobacter Pylori Eradication Therapy in both Erosive and Non-erosive Gastritis — A Prospective Study

2014 ◽  
Vol 4 (1) ◽  
pp. 15-20
Author(s):  
Mohammad Quamrul Hasan ◽  
MM Shahin-ul-Islam ◽  
Shahidul Hasan Mollick ◽  
Irin Perveen ◽  
ASMA Raihan
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Prospective Study ◽  
Rapid Urease Test ◽  
Erosive Gastritis ◽  
Endoscopic Findings ◽  
Urease Test ◽  
H Pylori ◽  
Gastric Malignancy ◽  
Better Than

Background: Infection with Helicobacter pylori (H. pylori) is a recognized cause of peptic ulcer and gastritis. Persistence of infection is a definite risk factor for gastric malignancy. Healing of gastritis after eradication of H. pylori reduces the risks of peptic ulcer disease and gastric malignancy. Objectives: To find out the relationship of H. pylori with erosive and nonerosive gastritis, the effect of anti-H. pylori therapy and to compare the effects of anti-H. pylori therapy between two types of gastritis. Materials and Methods: This prospective study was done in the Gastroenterology department of Bangabandhu Sheikh Mujib Medical University, Dhaka from June 2008 to May 2009. One hundred eighty dyspeptic patients were enrolled for the study. Patients with gastritis diagnosed by endoscopy underwent rapid urease test (RUT). RUT positive patients were considered to have H. pylori infection and were treated with triple therapy (omeprazole, amoxycillin and metronidiazole) for 14 days. Treatment responses were assessed by clinical history and also by endoscopic biopsy and RUT. Results of endoscopic findings and RUT after treatment were compared with pretreatment status. Results: Seventy patients completed the treatment and finally could be assessed. Endoscopic findings of 70 patients revealed that 56 (80%) patients had erosive gastritis and 14 (20%) patients had nonerosive gastritis. After treatment, 47 (67.1%) lesions became normal, 16 (22.9%) remained erosive and 7 (10%) non-erosive as before. Out of 14 non-erosive diseases, 7 became normal, while out of 56 erosive diseases 40 became normal. The erosive group responded significantly better than the non-erosive group (c2=32.766, p<0.001). Fifty nine (84.3%) patients with gastritis showed negative urease test after treatment. Conclusion: Strong relation between H. pylori infection and gastritis was found. Majority were antral erosive gastritis. Erosive group responded better than non-erosive group. DOI: http://dx.doi.org/10.3329/jemc.v4i1.18063 J Enam Med Col 2014; 4(1): 15-20

scholarly journals Antral Brushings and Biopsies for GastricCampylobacter pylori: A Comparative Study of a Rapid Urease Test, Culture and Histology

1989 ◽  
Vol 3 (3) ◽  
pp. 91-94
Author(s):  
Howard M. Estrin ◽  
Medhat O. Hassan ◽  
Howard S. Carr ◽  
Steven J. Czinn
Keyword(s):  
Prospective Study ◽  
Diagnostic Tests ◽  
Campylobacter Pylori ◽  
Rapid Urease Test ◽  
Gastric Mucus ◽  
Test Culture ◽  
Urease Test ◽  
Gastric Biopsies ◽  
A Prospective Study ◽  
Cytology Brush

A prospective study was conducted to assess the sensitivity and specificity of diagnostic tests for Campylobacter pylori in 60 unselected patients referred for gastroscopy. Urease testing was performed on samples of gastric mucus obtained by a disposable cytology brush and on gastric mucosal biopsies. The combination of the brush urease test and biopsy urease test had a sensitivity of 78% and a specificity of 96%. The combination of the brush urease test and examination of gastric biopsies for curved bacilli had a sensitivity of 96% and a specificity of 96%. The combination of the brush urease and biopsy urease tests will rapidly identify the majority of patients withC pylori. This test is rapid, easy, sensitive and inexpensive. If a pathologist is available, the addition of histologic review forC pyloriin combination with the brush urease test, will identify virtually all patients withC pyloriwithout the need for culturing this fastidious organism.

Frequency of rdx A Deletion Mutation Conferring Metronidazole Resistance in Helicobacter pylori

2020 ◽  
Vol 29 (3) ◽  
pp. 59-64
Author(s):  
Hanaa M. El Maghraby ◽  
Samar Mohaseb
Keyword(s):  
Helicobacter Pylori ◽  
Epigastric Pain ◽  
Deletion Mutation ◽  
Rapid Urease Test ◽  
Rrna Gene ◽  
University Hospitals ◽  
Metronidazole Resistance ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori

Background: Metronidazole is one of the antimicrobial drugs that can be used in combination with other drugs for eradication of Helicobacter pylori (H. pylori).Unfortunately, metronidazole resistance in H. plori is an increasing health problem which may be attributed to inactivation of many genes as rdx A gene. Objective: To determine the frequency of rdx A deletion mutation in H. pylori detected in infected patients attending at the Gastroenterology Unit, Zagazig University Hospitals. Methodology: Two gastric biopsies were taken from each enrolled patient by endoscopy. H.pylori detection was done by rapid urease test and polymerase chain reaction (PCR) amplification of 16S rRNA gene. Deletion mutation in rdx A gene was detected by conventional PCR. Results: Out of 134 doubled gastric biopsies obtained from 134 patients, 52.2% were positive for H. pylori. Epigastric pain, vomiting and gastritis were significantly associated with detection of H. pylori infection (p˂ 0.05). Deletion mutation of rdx A gene was detected in 28.6% of H. pylori positive specimens obtained from infected patients. Conclusion: Deletion mutation of rdx A gene is a frequent determinant of rdx A inactivation conferring metronidazole resistance among H. pylori.

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