The effect of the biopsy number on sensitivity and specificity of ultra-rapid urease test in detecting Helicobacter pylori: A prospective study

2001 ◽  
Vol 53 (5) ◽  
pp. AB210
Keyword(s):  
Helicobacter Pylori ◽  
Prospective Study ◽  
Sensitivity And Specificity ◽  
Rapid Urease Test ◽  
Urease Test ◽  
A Prospective Study

scholarly journals Assessment of medication adherence in Helicobacter pylori positive patients on standard triple therapy: a prospective study

2020 ◽  
Vol 9 (7) ◽  
pp. 1100
Author(s):  
Aleena Francis ◽  
Arathi S. Nair ◽  
Limi Joseph Gomez ◽  
Bushra Abdul Rahim ◽  
Soumya R. V. ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Medication Adherence ◽  
Prospective Study ◽  
Triple Therapy ◽  
Study Patient ◽  
Rapid Urease Test ◽  
Adherence Score ◽  
Standard Triple Therapy ◽  
Urease Test ◽  
A Prospective Study

Background: In the current study patient compliance to the standard triple therapy were assessed. The objective behind this research was to assess the patient medication adherence to the standard triple therapy in Helicobacter pylori infection.Methods: A prospective study was carried out for a period of 1 year and samples were taken from the gastroenterology department. Patient who was RUT (rapid urease test) positive by endoscopy were considered as H. pylori infected and they were prescribed with standard triple therapy. This regimen involves amoxicillin 1000 mg and clarithromycin 500 mg and proton pump inhibitor (PPI) twice a day for 14 days. Adherence to this triple therapy was assessed during the study. Medication adherence assessed using Morisky, Green and Levine (MGL) adherence scale. MGL adherence questionnaire was given to patients during first week and second week of therapy. The patients will be counselled regarding the drug administration, drug related problems and the infection. They were also provided with written instructions in leaflets. 88 patients were analyzed.Results: In this study, 84 patients had a good adherence 95.5%; but other 4.5% didn't fully comply with the physician's order. After the first week of standard triple therapy, adherence was increased to a mean score from 2.193±0.1301 to 3.5227±0.0704 in the second week. The medication adherence score significantly increased along with patient counselling.Conclusions: Medication adherence was improved in the 14 days course along with patient education helped to comply with the standard triple therapy.

scholarly journals Prospective study to evaluate the number and the location of biopsies in rapid urease test for diagnosis of Helicobacter Pylori

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Antoine Abou Rached ◽  
Jowana Saba ◽  
Cesar Yaghi ◽  
Joyce Sanyour ◽  
Ahmad El Hajjar ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Sensitivity And Specificity ◽  
Predictive Value ◽  
Rapid Test ◽  
Rapid Urease Test ◽  
Ulcer Disease ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori ◽  
Antral Biopsy

Helicobacter pylori (H. pylori) can cause a wide variety of illnesses such as peptic ulcer disease, gastric adenocarcinoma and mucosa-associated lymphoid tissue (MALT) lymphoma. The diagnosis and eradication of H. pylori are crucial. The diagnosis of H. pylori is usually based on the rapid urease test (RUT) and gastric antral biopsy for histology. The aim of this study is to evaluate the numbers of needed biopsies and their location (antrum/fundus) to obtain optimal result for the diagnosis of H. pylori. Three hundred fifty consecutive patients were recruited, 210 fulfill the inclusion criteria and had nine gastric biopsies for the detection of H. pylori infection: two antral for the first RUT (RUT1), one antral and one fundic for the second (RUT2), one antral for the third (RUT3) and two antral with two fundic for histology (HES, Giemsa, PAS). The reading of the 3 types of RUT was performed at 1 hour, 3 hours and 24 hours and biopsies were read by two experienced pathologists not informed about the result of RUT. Results of RUT were considered positive if H. pylori was found on histology of at least one biopsy. The RUT1 at 1h, 3h and 24h has a sensitivity of 72%, 82% and 89% and a specificity of 100%, 99% and 87% respectively. The positive predictive value (PPV) was 100%, 99% and 85% respectively and the negative predictive value (NPV) of 81%, 87% and 90%. The RUT2 at 1h, 3h and 24h, respectively, had a sensitivity of 86%, 87% and 91% and a specificity of 99%, 97% and 90%. The PPV was 99%, 96% and 88% and NPV of 89%, 90%, 94%. The RUT3 at 1h, 3h and 24h, respectively, had a sensitivity of 70%, 74% and 84% and a specificity of 99%, 99% and 94%. The PPV was 99%, 99% and 92% and NPV of 79%, 81% and 87%. The best sensitivity and specificity were obtained for RUT1 read at 3h, for RUT2 read 1h and 3h, and the RUT3 read at 24h.This study demonstrates that the best sensitivity and specificity of rapid test for urease is obtained when fundic plus antral biopsy specimens are used with a reading time at 3 hours.

scholarly journals Helicobacter Pylori Eradication Therapy in both Erosive and Non-erosive Gastritis — A Prospective Study

2014 ◽  
Vol 4 (1) ◽  
pp. 15-20
Author(s):  
Mohammad Quamrul Hasan ◽  
MM Shahin-ul-Islam ◽  
Shahidul Hasan Mollick ◽  
Irin Perveen ◽  
ASMA Raihan
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Prospective Study ◽  
Rapid Urease Test ◽  
Erosive Gastritis ◽  
Endoscopic Findings ◽  
Urease Test ◽  
H Pylori ◽  
Gastric Malignancy ◽  
Better Than

Background: Infection with Helicobacter pylori (H. pylori) is a recognized cause of peptic ulcer and gastritis. Persistence of infection is a definite risk factor for gastric malignancy. Healing of gastritis after eradication of H. pylori reduces the risks of peptic ulcer disease and gastric malignancy. Objectives: To find out the relationship of H. pylori with erosive and nonerosive gastritis, the effect of anti-H. pylori therapy and to compare the effects of anti-H. pylori therapy between two types of gastritis. Materials and Methods: This prospective study was done in the Gastroenterology department of Bangabandhu Sheikh Mujib Medical University, Dhaka from June 2008 to May 2009. One hundred eighty dyspeptic patients were enrolled for the study. Patients with gastritis diagnosed by endoscopy underwent rapid urease test (RUT). RUT positive patients were considered to have H. pylori infection and were treated with triple therapy (omeprazole, amoxycillin and metronidiazole) for 14 days. Treatment responses were assessed by clinical history and also by endoscopic biopsy and RUT. Results of endoscopic findings and RUT after treatment were compared with pretreatment status. Results: Seventy patients completed the treatment and finally could be assessed. Endoscopic findings of 70 patients revealed that 56 (80%) patients had erosive gastritis and 14 (20%) patients had nonerosive gastritis. After treatment, 47 (67.1%) lesions became normal, 16 (22.9%) remained erosive and 7 (10%) non-erosive as before. Out of 14 non-erosive diseases, 7 became normal, while out of 56 erosive diseases 40 became normal. The erosive group responded significantly better than the non-erosive group (c2=32.766, p<0.001). Fifty nine (84.3%) patients with gastritis showed negative urease test after treatment. Conclusion: Strong relation between H. pylori infection and gastritis was found. Majority were antral erosive gastritis. Erosive group responded better than non-erosive group. DOI: http://dx.doi.org/10.3329/jemc.v4i1.18063 J Enam Med Col 2014; 4(1): 15-20

scholarly journals FOCAL ENHANCED GASTRITIS AND MACROPHAGE MICROAGGREGATES IN THE GASTRIC MUCOSA: potential role in the differential diagnosis between Crohn’s disease and ulcerative colitis

2014 ◽  
Vol 51 (4) ◽  
pp. 276-282 ◽  
Author(s):  
Marcia Henriques de MAGALHÃES-COSTA ◽  
Beatriz Ribeiro dos REIS ◽  
Vera Lúcia Antunes CHAGAS ◽  
Tiago NUNES ◽  
Heitor Siffert Pereira de SOUZA ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Helicobacter Pylori ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Sensitivity And Specificity ◽  
Rapid Urease Test ◽  
Urease Test ◽  
High Prevalence ◽  
Focally Enhanced Gastritis ◽  
H Pylori

Context and Objectives Focally enhanced gastritis and macrophage microaggregates are found in the upper gastrointestinal involvement of Crohn’s disease, and may reflect an underlying defective innate immunity. These features, however, are also described in patients with Helicobacter pylori infection. The role of these gastric abnormalities in the diagnosis of Crohn’s disease was assessed in a population with high prevalence of H. pylori infection. Methods Thirty-seven Crohn’s disease, 26 ulcerative colitis, and 30 control patients were included. The H. pylori status was evaluated by the rapid urease test and histology. The presence of focally enhanced gastritis and macrophage microaggregates was recorded. Results Focally enhanced gastritis was present in 24% of Crohn’s disease patients, 4% of ulcerative colitis patients and 11.5% of controls, presenting an overall sensitivity and specificity for Crohn’s disease of 24% and 88%, respectively. Macrophage microaggregates were found in all groups, but were only detected in ulcerative colitis and controls in association with H. pylori infection, with an overall sensitivity and specificity for Crohn’s disease of 61% and 69%, respectively. In the absence of H. pylori infection, focally enhanced gastritis and macrophage microaggregates were significantly associated with Crohn’s disease (P<0.02 and P = 0.001 respectively). Conclusions Focally gastritis and macrophage microaggregates are suggestive of Crohn’s disease only in H. pylori-negative specimens. HEADINGS - Crohn’s disease. Ulcerative colitis. Gastritis. Macrophages. Helicobacter pylori.

scholarly journals Antral Brushings and Biopsies for GastricCampylobacter pylori: A Comparative Study of a Rapid Urease Test, Culture and Histology

1989 ◽  
Vol 3 (3) ◽  
pp. 91-94
Author(s):  
Howard M. Estrin ◽  
Medhat O. Hassan ◽  
Howard S. Carr ◽  
Steven J. Czinn
Keyword(s):  
Prospective Study ◽  
Diagnostic Tests ◽  
Campylobacter Pylori ◽  
Rapid Urease Test ◽  
Gastric Mucus ◽  
Test Culture ◽  
Urease Test ◽  
Gastric Biopsies ◽  
A Prospective Study ◽  
Cytology Brush

A prospective study was conducted to assess the sensitivity and specificity of diagnostic tests for Campylobacter pylori in 60 unselected patients referred for gastroscopy. Urease testing was performed on samples of gastric mucus obtained by a disposable cytology brush and on gastric mucosal biopsies. The combination of the brush urease test and biopsy urease test had a sensitivity of 78% and a specificity of 96%. The combination of the brush urease test and examination of gastric biopsies for curved bacilli had a sensitivity of 96% and a specificity of 96%. The combination of the brush urease and biopsy urease tests will rapidly identify the majority of patients withC pylori. This test is rapid, easy, sensitive and inexpensive. If a pathologist is available, the addition of histologic review forC pyloriin combination with the brush urease test, will identify virtually all patients withC pyloriwithout the need for culturing this fastidious organism.

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