Rat model of chronic tympanic membrane perforation: Ventilation tube with mitomycin C and dexamethasone

2016 ◽  
Vol 80 ◽  
pp. 61-68 ◽  
Author(s):  
Allen Y. Wang ◽  
Yi Shen ◽  
Lawrence J Liew ◽  
Jeffrey T. Wang ◽  
Magnus von Unge ◽  
...  
Keyword(s):  
Tympanic Membrane ◽  
Mitomycin C ◽  
Rat Model ◽  
Tympanic Membrane Perforation ◽  
Ventilation Tube ◽  
Membrane Perforation ◽  
Chronic Tympanic Membrane Perforation

scholarly journals Chronification of Tympanic Perforation with Mitomycin C in a Rat Model

2018 ◽  
Author(s):  
Tomás Esteban ◽  
Noemí M. Atucha ◽  
José María Moraleda ◽  
Joaquín García-Estañ ◽  
Carmen L. Insausti ◽  
...  
Keyword(s):  
Longitudinal Study ◽  
Tympanic Membrane ◽  
Mitomycin C ◽  
Rat Model ◽  
Healing Time ◽  
Tympanic Membrane Perforation ◽  
New Materials ◽  
Tympanic Membrane Perforations ◽  
Membrane Perforation ◽  
Chronic Tympanic Membrane Perforation

Background. A rat model of chronic tympanic membrane perforation was developed to be used in the search of new materials for the sealing of these perforations. Methods. A longitudinal study was carried out in rats subjected to incisional myringotomy followed by the application of mitomycin C alone or with dexamethasone. Rats were checked at days 3, 7, 10, 14 and weekly thereafter until perforation closure, for up to 6 months. Results. The addition of dexamethasone is a key component in order to obtain a chronic opening. Myringotomies treated with saline had a mean healing time of 8.5 days. At 8 weeks, 70.5% of these remained perforated and at 6 months this number fell to 21.4%. Conclusion. This technique is able to maintain more than 70% of tympanic membrane perforations patent for at least 8 weeks. This rat model is adequate for its use in preclinical or translational research.

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