scholarly journals Chronification of Tympanic Perforation with Mitomycin C in a Rat Model

2018 ◽  
Author(s):  
Tomás Esteban ◽  
Noemí M. Atucha ◽  
José María Moraleda ◽  
Joaquín García-Estañ ◽  
Carmen L. Insausti ◽  
...  
Keyword(s):  
Longitudinal Study ◽  
Tympanic Membrane ◽  
Mitomycin C ◽  
Rat Model ◽  
Healing Time ◽  
Tympanic Membrane Perforation ◽  
New Materials ◽  
Tympanic Membrane Perforations ◽  
Membrane Perforation ◽  
Chronic Tympanic Membrane Perforation

Background. A rat model of chronic tympanic membrane perforation was developed to be used in the search of new materials for the sealing of these perforations. Methods. A longitudinal study was carried out in rats subjected to incisional myringotomy followed by the application of mitomycin C alone or with dexamethasone. Rats were checked at days 3, 7, 10, 14 and weekly thereafter until perforation closure, for up to 6 months. Results. The addition of dexamethasone is a key component in order to obtain a chronic opening. Myringotomies treated with saline had a mean healing time of 8.5 days. At 8 weeks, 70.5% of these remained perforated and at 6 months this number fell to 21.4%. Conclusion. This technique is able to maintain more than 70% of tympanic membrane perforations patent for at least 8 weeks. This rat model is adequate for its use in preclinical or translational research.

Platelet-rich plasma improves healing of tympanic membrane perforations: experimental study

2008 ◽  
Vol 123 (5) ◽  
pp. 482-487 ◽  
Author(s):  
E Erkilet ◽  
M Koyuncu ◽  
S Atmaca ◽  
M Yarim
Keyword(s):  
Tympanic Membrane ◽  
Histopathological Examination ◽  
Platelet Rich Plasma ◽  
Healing Time ◽  
Tympanic Membrane Perforation ◽  
Control Group ◽  
Tympanic Membrane Perforations ◽  
Significant Difference ◽  
Membrane Perforation ◽  
Tympanic Membranes

AbstractObjective:The aim of this study was to investigate the effect of local application of platelet-rich plasma to perforated rat tympanic membranes, in terms of healing time and histopathological outcome.Methods:Eighty-eight tympanic membranes of 44 rats were given a standard 3 mm perforation, and platelet-rich plasma was applied to the right tympanic membrane perforations. The left tympanic membranes were left to heal spontaneously, as controls. The 44 rats were divided into two groups. In group one, comprising 20 rats, daily otomicroscopic examination of the tympanic membrane perforations was performed. The 24 rats in group two were subdivided into four subgroups of six rats each; these subgroups were sacrificed sequentially on days three, seven, 14 and 28 for histopathological examination, regardless of tympanic membrane healing stage.Results:In group one, the mean tympanic membrane healing times for tympanic membrane perforations receiving platelet-rich plasma and controls were respectively 10.2 ± 2.1 and 13.0 ± 2.9 days (mean ± standard deviation). This difference was statistically significant (p < 0.001). In group two, histopathological evaluation of tympanic membrane perforation healing at days three, seven, 14 and 28 did not reveal any statistically significant difference, individually or within the four groups as a whole.Conclusion:These findings suggest that earlier healing of tympanic membrane perforations occurred in the platelet-rich plasma group compared with the control group. These findings suggest that platelet-rich plasma is effective in accelerating tympanic membrane perforation healing, and that it may be effective in human subjects, particularly as it is an autologous material.

Regeneration of the tympanic membrane using fibroblast growth factor-2

2018 ◽  
Vol 132 (06) ◽  
pp. 470-478 ◽  
Author(s):  
Z-C Lou ◽  
Z-H Lou ◽  
J Xiao
Keyword(s):  
Growth Factor ◽  
Success Rate ◽  
Tympanic Membrane ◽  
Fibroblast Growth Factor ◽  
Tympanic Membrane Perforation ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Tympanic Membrane Perforations ◽  
Membrane Perforation ◽  
Chronic Tympanic Membrane Perforation

AbstractObjectiveA systematic review was conducted to investigate the effectiveness of fibroblast growth factor-2 on the regeneration of tympanic membrane perforation.MethodsThe PubMed database was searched for relevant studies. Experimental studies, human randomised controlled trials, prospective single-arm studies and retrospective studies reporting acute and chronic tympanic membrane perforations in relation to two healing outcomes (success rate and closure time), were selected.ResultsAll 11 clinical studies investigating the effect of fibroblast growth factor-2 on traumatic tympanic membrane perforations in humans reported a success rate of 89.3–100 per cent, with a closure time of around 2 weeks. Three studies of fibroblast growth factor-2 combined with Gelfoam showed that the success rate of chronic tympanic membrane perforation was 83–98.1 per cent in the fibroblast growth factor-2 group, but 10 per cent in the gelatine sponge groups.ConclusionFibroblast growth factor-2 with or without biological material patching promotes regeneration in cases of acute and chronic tympanic membrane perforation, and is safe and efficient. However, the best dosage, application time and administration pathway of fibroblast growth factor-2 are still to be elucidated.

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