In vitro evaluation of the therapeutic potential of Anatolian kermes oak (Quercus coccifera L.) as an alternative wound healing agent

Human saliva is a complex body fluid with more than 3000 different identified proteins. Besides rheological and lubricating properties, saliva supports wound healing and acts as an antimicrobial barrier. TFF peptides are secreted from the mucous acini of the major and minor salivary glands and are typical constituents of normal saliva; TFF3 being the predominant peptide compared with TFF1 and TFF2. Only TFF3 is easily detectable by Western blotting. It occurs in two forms, a disulfide-linked homodimer (Mr: 13k) and a high-molecular-mass heterodimer with IgG Fc binding protein (FCGBP). TFF peptides are secretory lectins known for their protective effects in mucous epithelia; the TFF3 dimer probably has wound-healing properties due to its weak motogenic effect. There are multiple indications that FCGBP and TFF3-FCGBP play a key role in the innate immune defense of mucous epithelia. In addition, homodimeric TFF3 interacts in vitro with the salivary agglutinin DMBT1gp340. Here, the protective roles of TFF peptides, FCGBP, and DMBT1gp340 in saliva are discussed. TFF peptides are also used to reduce radiotherapy- or chemotherapy-induced oral mucositis. Thus, TFF peptides, FCGBP, and DMBT1gp340 are promising candidates for better formulations of artificial saliva, particularly improving wound healing and antimicrobial effects even in the esophagus.

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Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies

Clinical Oral Investigations ◽

10.1007/s00784-019-03156-9 ◽

2019 ◽

Vol 24 (2) ◽

pp. 569-584 ◽

Cited By ~ 9

Author(s):

Franz-Josef Strauss ◽

Jila Nasirzade ◽

Zahra Kargarpoor ◽

Alexandra Stähli ◽

Reinhard Gruber

Keyword(s):

Systematic Review ◽

Cell Proliferation ◽

Wound Healing ◽

Bone Regeneration ◽

Therapeutic Potential ◽

In Vitro Studies ◽

Bioactive Molecules ◽

Platelet Rich Fibrin ◽

Regenerative Dentistry

Abstract Objective To systematically assess the effects of platelet-rich fibrin (PRF) on in vitro cellular behavior. Methods A systematic electronic search using MEDLINE database was performed. In vitro studies using PRF were considered and articles published up to June 31, 2018 were screened. Eligible studies were selected based on the use of human PRF. Results In total, 1746 titles were identified with the search terms, from these 37 met the inclusion criteria and were chosen for data extraction. In addition, 16 new studies, mainly published in 2019, were also included in the analysis resulting in 53 studies. No meta-analysis could be performed due to the heterogeneity of study designs. Included studies show that PRF enhances proliferation, migration, adhesion, and osteogenic differentiation on a variety of cell types along with cell signaling activation. Furthermore, PRF reduces inflammation, suppresses osteoclastogenesis, and increases the expression of various growth factors in mesenchymal cells. Summary and conclusions Despite some notable differences of the studies, the overall findings suggest a positive effect of PRF on cell proliferation, migration, adhesion, differentiation, and inflammation pointing towards a therapeutic potential in regenerative dentistry. Clinical relevance PRF serves as a reservoir of bioactive molecules to support wound healing and bone regeneration. Although the cellular mechanisms by which PRF supports the clinical outcomes remain unclear, in vitro research provides possible explanations. This systematic review aims to provide an update of the existing research on how PRF affects basic physiological processes in vitro. The overall findings suggest that PRF induces cell proliferation, migration, adhesion, and differentiation along with possessing anti-inflammatory properties further supporting its therapeutic potential in wound healing and bone regeneration.

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Wound healing features of Prosopis farcta: in vitro evaluation of antibacterial, antioxidant, proliferative and angiogenic properties

Gene Reports ◽

10.1016/j.genrep.2019.100482 ◽

2019 ◽

Vol 17 ◽

pp. 100482 ◽

Cited By ~ 1

Author(s):

Razieh Noroozi ◽

Ehsan Sadeghi ◽

Hassan Yousefi ◽

Mohammad Taheri ◽

Parisa Sarabi ◽

...

Keyword(s):

Wound Healing ◽

In Vitro Evaluation ◽

Prosopis Farcta

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Optimization of production, biochemical characterization and in vitro evaluation of the therapeutic potential of fibrinolytic enzymes from a new Bacillus amyloliquefaciens

Macromolecular Research ◽

10.1007/s13233-016-4089-2 ◽

2016 ◽

Vol 24 (7) ◽

pp. 587-595 ◽

Cited By ~ 5

Author(s):

Fabiana América Silva Dantas de Souza ◽

Amanda Emmanuelle Sales ◽

Pablo Eugênio Costa e Silva ◽

Raquel Pedrosa Bezerra ◽

Germana Michelle de Medeiros e Silva ◽

...

Keyword(s):

Bacillus Amyloliquefaciens ◽

Biochemical Characterization ◽

Therapeutic Potential ◽

Fibrinolytic Enzymes ◽

In Vitro Evaluation

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Toxicity, mutagenicity and stability assessment of simply produced electrolyzed water as a wound healing agent in vitro

Human & Experimental Toxicology ◽

10.1177/0960327120952151 ◽

2020 ◽

pp. 096032712095215 ◽

Cited By ~ 1

Author(s):

R Reis ◽

H Sipahi ◽

O Dinc ◽

T Kavaz ◽

M Charehsaz ◽

...

Keyword(s):

Wound Healing ◽

Mutagenic Activity ◽

Tap Water ◽

L929 Cell ◽

Metabolic Activation ◽

Strong Acid ◽

Storage Conditions ◽

Electrolyzed Water ◽

Healing Agent

Over the last decade, electrolyzed water (EW) produced by salt and tap water has gained importance due to its antimicrobial effects. Regarding to chlorine-based compounds, EW also used in post-harvest safety of food processing and sterilization of surfaces. The latest studies suggested that EW might act as wound healing agent due to anti-infective and cell proliferative properties. In this study, we evaluated acute contact cytotoxicity in L929 mice fibroblast cells and wound healing activity of EWs in vitro. In addition, mutagenic activity was evaluated by Ames test with and without metabolic activation by S9 fraction and the stability profile of freshly prepared EWs has been followed up. According to the results, strong acid (StAEW) and mixed EW (MEW) showed dose-dependent cytotoxicity due to possible high HOCl concentration, while slightly acidic and catholyte EW (CEW) were not cytotoxic even applied directly for 30 sec. Further, StAEW and CEW showed a significant increase in L929 cell migration in scratch assay. Likewise, with/ without metabolic activation, neither of EWs had shown mutagenic profile in TA 98 and TA100 strains of Salmonella typhimurium. Follow-up of ORP (oxidation-reduction potential), pH and FCC (free chlorine concentration) showed that temperature and light were important storage conditions to maintain a stable profile particularly for ORP and FCC, which are the most important indicators for biological activity of EW. According to the present findings, it can be suggested that particularly StAEW, may represent a valuable wound healing agent with an achievable, economical and easy production system when stored under proper conditions.

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