Bactericidal efficacy of slightly acidic electrolyzed water (SAEW) against Listeria monocytogenes planktonic cells and biofilm on food-contact surfaces

In the present study, the bactericidal efficacy of slightly acidic electrolyzed water (SAEW) against L. monocytogenes planktonic cells and biofilm on food-contact surfaces including stainless steel and glass was systematically evaluated. The results showed that SAEW (pH of 5.09 and available chlorine concentration (ACC) of 60.33 mg/L) could kill L. monocytogenes on food-contact surfaces completely in 30 s, whose disinfection efficacy is equal to that of NaClO solutions (pH of 9.23 and ACC of 253.53 mg/L). The results showed that long exposure time and high ACC contributed to the enhancement of the disinfection efficacy of SAEW on L. monocytogenes on food-contact surfaces. Moreover, the log reduction of SAEW treatment presented an increasing tendency within the prolonging of treatment time when SAEW was used to remove the L. monocytogenes biofilm formed on stainless steel and glass surfaces, which suggested that SAEW could remove L. monocytogenes biofilm effectively and its disinfection efficacy is equal to (in case of stainless steel) or higher than (in case of glass) that of high ACC of NaClO solutions. In addition, the results of the crystal violet staining and scanning electron microscopy (SEM) also demonstrated that SAEW treatment could remove the L. monocytogenes biofilm on food-contact surfaces.

Slightly Acidic Electrolyzed Water to Remove Methylobacterium mesophilicum, Rhodotorula mucilaginosa and Cladosporium cladosporioides in Households

(1) Background: Slightly acidic electrolyzed water (SAEW) is an effective and safe sterilizing solution. Its active component is hypochlorous acid (HOCl) which has been proved to exhibit a strong disinfectant activity. In this research we evaluated the effectiveness of SAEW in the removal of Methylobacterium mesophilicum, Rhodotorula mucilaginosa and Cladosporium cladosporioides, responsible for pink-colored biofilm and black mold in households. (2) Methods: Two concentrations of SAEW, 20 mg/L and 40 mg/L, were tested against M. mesophilicum, R. mucilaginosa and C. cladosporioides. In vitro experiments and mesh experiments were conducted to test the effectiveness of SAEW. (3) Results: The test results showed that 40 mg/L SAEW was effective in removing R. mucilaginosa and C. cladosporioides, with the population decreasing by approximately two orders of magnitude. For M. mesophilicum, resistance towards SAEW was observed; to obtain a 1.3 order of magnitude decrease in bacterial population, washing 5 times with 40 mg/L SAEW was necessary. Mesh experiments showed that SAEW can remove black mold; (4) Conclusions: Overall results indicated that SAEW was particularly effective for R. mucilaginosa and C. cladosporioides species commonly found in Japanese households.

Research Trends on the Application of Electrolyzed Water in Food Preservation and Sanitation

Electrolyzed water (EW) has been proposed as a novel promising sanitizer and cleaner in recent years. It is an effective antimicrobial and antibiofilm agent that has several advantages of being on the spot, environmentally friendly, cheap, and safe for human beings. Therefore, EW has been applied widely in various fields, including agriculture, food sanitation, livestock management, medical disinfection, clinical, and other fields using antibacterial technology. Currently, EW has potential significance for high-risk settings in hospitals and other clinical facilities. The research focus has been shifted toward the application of slightly acidic EW as more effective with some supplemental chemical and physical treatment methods such as ultraviolet radiations and ultrasound. This review article summarizes the possible mechanism of action and highlights the latest research studies in antimicrobial applications.

Efficacy of slightly acidic electrolyzed water on inactivation of Cronobacter sakazakii and biofilm cell

The disinfection efficacy and mechanism of slightly acid electrolyzed water (SAEW) on Cronobacter sakazakii were investigated. SAEW solutions in three concentrations were carried on C. sakazakii which decreased in a range of 23%-55% in 2 minutes. The propidium iodide (PI) uptake and electronic microscopy (SEM) images indicated that SAEW treatment damaged cell integrity and changed membrane permeability with leaking nucleic acid (109.7%), intercellular protein (692.3%) and K + (53.6%). It was accompanied with lower ability of biofilm formation. SAEW treatment reduced the activity of SOD and CAT from 100.73 U/mgprot and 114.18 U/mgprot to 50.03 U/mgprot and 50.13 U/mgprot, respectively. It lowered down the gene expression of response regulator (katG, rpoS, phoP, glpK,dacC and CSK29544_RS05515 ) which made C. sakazakii failed to repair osmotic stress-induced damage and inhibited their biofilm formation. These findings provide an understanding of associations between bacterial genotype and phenotype induced by SAEW.