miR-185-5p alleviates CCI-induced neuropathic pain by repressing NLRP3 inflammasome through dual targeting MyD88 and CXCR4

2022 ◽  
Vol 104 ◽  
pp. 108508
Author(s):  
Airu Huang ◽  
Ling Ji ◽  
Yilong Huang ◽  
Qian Yu ◽  
Yufeng Li
Keyword(s):  
Neuropathic Pain ◽  
Nlrp3 Inflammasome ◽  
Dual Targeting

Salidroside alleviates diabetic neuropathic pain through regulation of the AMPK-NLRP3 inflammasome axis

2021 ◽  
Vol 416 ◽  
pp. 115468
Author(s):  
Tao Zheng ◽  
Qibin Wang ◽  
Fang Bian ◽  
Yan Zhao ◽  
Weidong Ma ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Nlrp3 Inflammasome ◽  
Diabetic Neuropathic Pain

scholarly journals miRNA-23a/CXCR4 regulates neuropathic pain via directly targeting TXNIP/NLRP3 inflammasome axis

2018 ◽  
Vol 15 (1) ◽  
Author(s):  
Zhiqiang Pan ◽  
Qun Shan ◽  
Pan Gu ◽  
Xiao Min Wang ◽  
Lydia Wai Tai ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Nlrp3 Inflammasome

Dual Targeting of Autophagy and NF-κB Pathway by PPARγ Contributes to the Inhibitory Effect of Demethoxycurcumin on NLRP3 Inflammasome Priming

2021 ◽  
Vol 14 ◽  
Author(s):  
Jing Tang ◽  
Xiaoxue Tan ◽  
Xiangmi Huang ◽  
Jie Zhang ◽  
Liang Chen ◽  
...  
Keyword(s):  
Nlrp3 Inflammasome ◽  
Nuclear Translocation ◽  
Inhibitory Effect ◽  
Caspase 1 ◽  
Dual Targeting ◽  
Transmission Electron ◽  
Subsequent Activation ◽  
Natural Derivative ◽  
Pparγ Expression

Background: Demethoxycurcumin (DMC), a natural derivative of curcumin, has anti-inflammatory activities. However, the mechanism has not been fully elucidated. Objective: The aim of the current study was to investigate the role of DMC on NLRP3 inflammasome priming. Methods: Protein expression was quantified by western blotting. Inflammatory cytokines were measured by ELISA. Autophagosomes were evaluated by transmission electron microscopy. Results: DMC inhibited LPS-stimulated NLRP3, pro-caspase-1, and pro-IL-1β expression. Meanwhile, DMC diminished NLRP3-dependent IL-1β maturation, caspase-1 activation, IL-1β and IL-18 production caused by LPS plus ATP. Moreover, DMC induced autophagy and autophagy inhibitor 3-MA abrogated the role of DMC on NLRP3 inflammasome priming and subsequent activation. DMC also inhibited LPS-stimulated phosphorylation and nuclear translocation of p65 NF-κB. Additionally, DMC significantly increased the PPARγ expression and the effects of DMC in NF-κB inhibition, autophagy, and NLRP3 inflammasome priming were abrogated by specific PPARγ antagonist T0070907. Conclusion: The evidence presented here has confirmed that DMC increases PPARγ expression, resulting in autophagy and NF-κB inhibition, and subsequently inhibits LPS-induced NLRP3 inflammasome priming and subsequent activation.

Mechanism of Ginkgolide B Amelioration of Neuropathic Pain Induced by Chronic Constriction Injury

2021 ◽  
Vol 19 (3) ◽  
pp. 359-365
Author(s):  
Guizhen Yan ◽  
Aobo Ma ◽  
Man Huang ◽  
Yuan Zhang
Keyword(s):  
Neuropathic Pain ◽  
Protein Expression ◽  
Nlrp3 Inflammasome ◽  
Chronic Constriction Injury ◽  
Nerve Fibers ◽  
Fibrous Structure ◽  
Receptor Protein ◽  
Inflammasome Activation ◽  
Ginkgolide B ◽  
Nlrp3 Inflammasome Activation

Activation of NOD-like receptor protein 3 (NLRP3) inflammasome is implicated in the pathogenesis of neuropathic pain. Ginkgolide B contributes to the suppression of NLRP3 inflammasome activation to prevent hypoxic-ischemic brain injury. However, the role of ginkgolide B on neuropathic pain has not been reported yet. We have shown that administration of ginkgolide B lowered pain threshold measured by paw-withdrawal threshold and paw-withdrawal latency in rats subjected to chronic constriction injury. Nerve fibers in rats postchronic constriction injury were swollen, and the fibrous structure was disordered. Treatment with ginkgolide B attenuated the nerve fiber swelling and reduced the disordered fibrous structure. Ginkgolide B dosage dependently attenuated chronic constriction injury-induced increase of proinflammatory cytokines. Protein expression of NLRP3 and its downstream targets (caspase 1 and IL-1β) were increased by chronic constriction injury and reduced by ginkgolide B. Lastly, ginkgolide B counteracted with the promotive effects of chronic constriction injury on protein expression of TLR4 and p-NF-κB. In conclusion, ginkgolide B demonstrated anti-inflammatory and antinociceptive effects in rats' model with neuropathic pain by suppression of TLR4-NF-κB-mediated NLRP3 inflammasome activation.

scholarly journals Neuropathic Pain Phenotype Does Not Involve the NLRP3 Inflammasome and Its End Product Interleukin-1β in the Mice Spared Nerve Injury Model

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0133707 ◽  
Author(s):  
Verdad Curto-Reyes ◽  
Guylène Kirschmann ◽  
Marie Pertin ◽  
Stephan K. Drexler ◽  
Isabelle Decosterd ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Nerve Injury ◽  
Nlrp3 Inflammasome ◽  
Interleukin 1Β ◽  
Spared Nerve Injury ◽  
Injury Model

scholarly journals Divanillyl sulfone suppresses NLRP3 inflammasome activation via inducing mitophagy to ameliorate chronic neuropathic pain in mice

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Shuai Shao ◽  
Cheng-Bo Xu ◽  
Cheng-Juan Chen ◽  
Gao-Na Shi ◽  
Qing-Lan Guo ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Nlrp3 Inflammasome ◽  
Intrathecal Injection ◽  
Beclin 1 ◽  
Inflammasome Activation ◽  
Chronic Neuropathic Pain ◽  
Withdrawal Threshold ◽  
Caspase 1 ◽  
Nlrp3 Inflammasome Activation ◽  
Related Proteins

Abstract Background Chronic neuropathic pain is a frequent sequel to peripheral nerve injury and maladaptive nervous system function. Divanillyl sulfone (DS), a novel structural derivative of 4,4′-dihydroxydibenzyl sulfoxide from a traditional Chinese medicine Gastrodia elata with anti-nociceptive effects, significantly alleviated neuropathic pain following intrathecal injection. Here, we aimed to investigate the underlying mechanisms of DS against neuropathic pain. Methods A chronic constrictive injury (CCI) mouse model of neuropathic pain induced by sciatic nerve ligation was performed to evaluate the effect of DS by measuring the limb withdrawal using Von Frey filament test. Immunofluorescence staining was used to assess the cell localizations and expressions of Iba-1, ASC, NLRP3, and ROS, the formation of autolysosome. The levels of NLRP3-related proteins (caspase-1, NLRP3, and IL-1β), mitophagy-related proteins (LC3, Beclin-1, and p62), and apoptosis-related proteins (Bcl-XL and Bax) were detected by Western blotting. The apoptosis of BV-2 cell and caspase activity were evaluated by flow cytometry. Results DS significantly alleviated the neuropathic pain by increasing the mechanical withdrawal threshold and inhibiting the activation of NLRP3 in CCI-induced model mice. Our findings indicated that DS promoted the mitophagy by increasing the LC3II and Beclin 1 and decreasing the levels of p62 protein in BV-2 cell. This is accompanied by the inhibition of NLRP3 activation, which was shown as inhibited the expression of NLRP3 in lysates as well as the secretion of mature caspase-1 p10 and IL-1β p17 in supernatants in cultured BV-2 microglia. In addition, DS could promote mitophagy-induced improvement of dysfunctional mitochondria by clearing intracellular ROS and restoring mitochondrial membrane potential. Conclusion Together, our findings demonstrated that DS ameliorate chronic neuropathic pain in mice by suppressing NLRP3 inflammasome activation induced by mitophagy in microglia. DS may be a promising therapeutic agent for chronic neuropathic pain.

NLRP3 Inflammasome Mediates Neurodegeneration in Rats With Chronic Neuropathic Pain

Shock ◽  
2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Changhe Ren ◽  
Milian Chen ◽  
Guo Mu ◽  
Suangchun Peng ◽  
Xiangbo Liu ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Nlrp3 Inflammasome ◽  
Chronic Neuropathic Pain

scholarly journals Albiflorin Attenuates Mood Disorders Under Neuropathic Pain State by Suppressing the Hippocampal NLRP3 Inflammasome Activation During Chronic Constriction Injury

2020 ◽  
Author(s):  
Pei Liu ◽  
Jianjun Chen ◽  
Shuai Ma ◽  
Jianjun Zhang ◽  
Jianyu Zhou
Keyword(s):  
Neuropathic Pain ◽  
Mood Disorders ◽  
Nlrp3 Inflammasome ◽  
Chronic Constriction Injury ◽  
Anxiety And Depression ◽  
Inflammasome Activation ◽  
Nuclear Factor ◽  
Nlrp3 Inflammasome Activation ◽  
Pain State ◽  
Neuropathic Pain State

Abstract Background Neuropathic pain is a multifaceted and ubiquitous disease across the globe. Mood disorders, such as anxiety and depression, are frequently observed in patients suffering from neuropathic pain. Both neuropathic pain and comorbid mood disorders seriously impact quality of life. Accumulated evidence shows that activation of the NOD-like receptor protein 3 (NLRP3) inflammasome is involved in the neuroinflammatory pathogenesis of neuropathic pain, anxiety, and depression. However, the role of the NLRP3 inflammasome in the pathological process of anxiety and depression under the neuropathic pain state has not been fully described. Albiflorin, a monoterpene glycoside, may be a potential regulator of the NLRP3 inflammasome, but it is not clear whether albiflorin relates to NLRP3 inflammasome activation. Methods We used a systematic pharmacological method to confirm whether the activation of the NLRP3 inflammasome in the hippocampus was involved in the development of neuropathic pain associated with mood disorders and whether albiflorin could be an effective treatment for these symptoms. Results The NLRP3 inflammasome contributed to the neuropathic pain and comorbid anxiety and depression-like behaviors induced by chronic constriction injury of the sciatic nerve, and albiflorin may relieve these symptoms via inhibition of the NLRP3 inflammasome activity. Moreover, albiflorin enhanced the translocation of the nuclear factor erythroid 2-related factor 2 into the nucleus and suppressed nuclear factor-kappa B activity in the hippocampus. Conclusions Albiflorin, as a potential therapeutic agent, might greatly improve the overall symptoms of neuropathic pain.

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